Cross talk in photochemical three-dimensional optical recording

10.1364/OL.29.002055Optics Letters29172055-2057OPLE

BioPhys4_08SoustovLO

Abstract -Here we compile and analyze the data on photoaggregation of a model protein carboanhydrase and the main eye lens proteins α -, β -, γ -crystallins under the action of pulsed UV irradiation from a Xe-Cl laser (308 nm) with broad variation of pulse energy density and repetition rate. The aggregation efficacy proves to be a nonlinear function of these parameters and protein concentration. A theoretical model is proposed that qualitatively explains the experimental data. It is shown that N-arm-truncated β A3-crystallin is more prone to UV-induced aggregation than the full-sized protein; such defects caused by mutation or aging may aggravate the development of lenticular opacity. Analyzed is the effect of some low-molecular compounds on the aggregation of β -crystallin and its mixture with α -crystallin. A combination of short peptides prepared on this basis markedly impedes crystallin aggregation and retards the development of UV-induced cataract in rats