Alchemilla Vulgaris L. kao potencijalni izvor prirodnih antioksidanata

The aim of this study was to examine the total phenolic and flavonoid content, and antioxidant activity of the methanol extract of Alchemilla vulgaris aerial parts, as a potential source of natural antioxidants. The obtained results showed that extract has high total phenolic content (590,22 mg GAE/g), wheras flavonoid content was lower (12,58 mg RU/g). Antioxidant activity was determined in vitro by DPPH· and ABTS+· reagents. The IC50 values of DPPH (5,40 μg/ml) and ABTS+· scavenging capacities (60,10 μg/ml) of methanol extract showed that tested extract has extremely strong antioxidant activity.Cilj ovog rada je bio ispitivanje antioksidativne aktivnosti, kao i količine ukupnih fenolnih jedinjenja i flavonoida u metanolnom ekstraktu biljke A. vulgaris L., kao potencijalnog izvora prirodnih antioksidanata. Ispitivani ekstrakt poseduje visok sadržaj ukupnih fenola (590,22 mg GA/g), dok je koncentracija flavonoida znatno manja (12,58 mg RU/g). Antioksidativna aktivnost je ispitivana in vitro DPPH i ABTS+· metodom, a IC50 vrednosti ukazuju da ispitivani ekstrakt pokazuje izuzetno visok stepen antioksidativne aktivnosti u poređenju sa referentnim fenolnim jedinjenjima, što ukazuje na mogućnost primene ekstrakta A. vulgaris u prehrambenoj industriji

Stabilnost ekstrakta korena suručice i efekat na sprečavanje lipidne oksidacije u mesu

Nowadays, consumers are demanding more natural foods, obliging the industry to include natural antioxidants in foods and synthetic antioxidants have been rejected because of their carcinogenicity. Natural antioxidants have been used instead of synthetic to retard lipid oxidation in foods to improve their quality and nutritional value. The aim of this work was to evaluate pH and termal stability, and effect on lipid oxidation in meat-model system of methanolic extract of Filipendula hexapetala Gilib. Results suggest that the stability of the extract is expressed in a variety of pH and thermal conditions. The root extract of F. hexapetala exerted a concentration-dependent antioxidant activity in a meat model system, lower than synthetic antioxidant BHT.Danas je u ishrani zastupljen veliki broj prirodnih proizvoda, tako da prehrambena industrija sve više koristi prirodne antioksidante, a sintetički antioksidanti se izbacuju iz upotrebe zbog njihove kancerogenosti. Prirodni antioksidanti se koriste umesto sintetičkih da uspore oksidaciju lipida, poboljšaju kvalitet i nutritivnu vrednost namirnica. Cilj ovog rada bio je određivanje pH i termalne stabilnosti, kao i efekta na lipidnu oksidaciju u mesu metanolskog ekstrakta korena biljke Filipendula hexapetala Gilib. Rezultati ukazuju da je stabilnost ekstrakta izražena u različitim pH i termalnim uslovima. Ekstrakt korena F. hexapetala pokazao je koncentraciono-zavisnu antioksidativnu aktivnost u meso model-sistemu, nešto nižu u odnosu na sintetički antioksidant BHT

Post-streptococcal glomerulonephritis

Post-streptococcal glomerulonephritis (PSGN) is a frequent cause of acute nephritis in children. This case study was done with the aim to point out that the infections caused by Group A streptococci, in spite of antibiotic era, are still present in the population. An 8-year old boy was admitted in our hospital with a two-day history of fewer, tonsillopharyngitis. After hospital admission, patient was treated with penicillin during the period of 10 days, antihypertensive medications (captopril, furosemide), including restricted diet of salt. After the treatment, patient became better. On demission it was found proteinuria and microhematuria PSGN is very serious disease, which leaves severe complications if the valid therapy with penicillin is not used in propriety time, during the recommended period of 10 days

Lythrum salicaria L. (Lythraceae) as a promising source of phenolic compounds in the modulation of oxidative stress: Comparison between aerial parts and root extracts

Lythrum salicaria is a flowering plant traditionally used for inflammatory diseases, gastrointestinal ailments, dysentery, and as astringent for external use. The aim of this study was to determine the phenolic content, antioxidant, cytotoxic, and antimicrobial activities of L. salicaria aerial part and root methanol extracts, as well as the protective effects against oxidative damage on DNA and on a cell-based model. Moreover, simulated in vitro digestion studies were performed to estimate the stability of individual compounds present in the extracts. L. salicaria extracts contained a significant amount of total phenolic compounds and several phenolic compounds including C-glycoside flavones, orientin, isoorientin, and vitexin, were identified in extracts. The extracts demonstrated high antioxidant potential in different in vitro methods, moderate antimicrobial properties, and low cytotoxicity on studied normal and cancer cell lines. Also, the extracts showed significant DNA protection and protection of cells against arsenite-induced oxidative stress. These results suggest that, although the aerial part of L. salicaria is in extensive use since ancient times as a medicinal plant, its root may also possess the valuable biological properties and both could be used as functional food ingredients, considering their good antioxidant and antigenotoxic activities, and stability during in vitro digestion

Application potential of biogenically synthesized silver nanoparticles using: Lythrum salicaria L. extracts as pharmaceuticals and catalysts for organic pollutant degradation

This study was designed to evaluate the optimal conditions for the eco-friendly synthesis of silver nanoparticles (AgNPs) using Lythrum salicaria L. (Lythraceae) aqueous extracts and their potential application and safe use. AgNPs synthesized using L. salicaria aerial parts (LSA-AgNPs) and root extract (LSR-AgNPs) were characterized by UV-Vis spectrophotometry, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM/EDS), and X-ray powder diffraction (XRPD). Dynamic light scattering (DLS) was used for the determination of the size distribution profiles of the obtained nanoparticles. Both L. salicaria extracts showed high phenolic content, while the flavone C-glucosides orientin, vitexin, and isovitexin were detected in extracts using HPLC. The synthesized AgNPs displayed growth inhibition of the tested bacteria and fungi in concentrations between 0.156 and 1.25 mg mL-1. The studied nanoparticles also showed antioxidant potential and gained selectivity at different concentrations on different cancer cell lines. Concentrations of LSA-AgNPs were found to be 20.5 and 12 μg mL-1 towards A431 and SVT2, respectively, while LSR-AgNPs were effective only against A431 cancer cells (62 μg mL-1). The hemolytic activity of LSA-AgNPs in concentrations up to 150 μg mL-1 was not observed, while LSR-AgNPs in the highest applied concentration hemolyzed 2.8% of erythrocytes. The degradation possibility of Congo red and 4-nitrophenol using LSA-AgNPs and LSR-AgNPs as catalysts was also proven. The results indicate that L. salicaria may be used for the eco-friendly synthesis of AgNPs with possible applications as antimicrobial and selective cytotoxic agents towards cancer cell lines, as well as in catalytic degradation of pollutants