Near-infrared spectroscopy of 1999 JU3, the target of the Hayabusa 2 mission

Context. Primitive asteroids contain complex organic material and ices relevant to the origin of life on Earth. These types of asteroids are the target of several-sample return missions to be launched in the next years. 1999 JU3 is the target of the Japanese Aerospace Exploration Agency's Hayabusa 2 mission. Aims. 1999 JU3 has been previously identified as a C-class asteroid. Spectroscopic observations at longer wavelengths will help to constrain its composition. Methods. We obtained spectroscopy of 1999 JU3 from 0.85 to 2.2 microns, with the 3.6 m Telescopio Nazionale Galileo using the low resolution mode of the Near Infrared Camera Spectrograph. Results. We present a near-infrared spectrum of 1999 JU3 from 0.85 to 2.2microns that is consistent with previously published spectra and with its C-type classification. Conclusions. Our spectrum confirms the primitive nature of 1999 JU3 and its interest as target of the sample-return mission Hayabusa 2.Comment: Research Note: 3 pages 1 Figure Received December 2012; accepted 4 March 201

The Evaluation of a Biologically Inspired Engineered MAV Wing compared to the Manduca Sexta Wing under Simulated Flapping Conditions

In recent years, researchers have expressed a vested interest in the concepts surrounding flapping wing micro air vehicles (FWMAVs) that are capable of both range and complex maneuvering. Most research in this arena has found itself concentrated on topics such as flapping dynamics and the associated fluid-structure interactions inherent in the motion; however there still remain a myriad of questions concerning the structural qualities intrinsic to the wings themselves. Using nature as the template for design, FWMAV wings were constructed using carbon fiber and Kapton and tested under simplified flapping conditions by analyzing ‘frozen’ digital images of the deformed wing by methods of photogrammetry. This flapping motion was achieved via the design and construction of a flapper that emulates several of the kinematic features that can be seen in naturally occurring flyers. The response to this motion was then compared to the inspiring specimen\u27s wings, the North American Hawkmoth (Manduca Sexta), under the same flapping conditions in order to identify some of the key features that nature has deemed necessary for successful flight. Results showed that though the engineered wing emulated several of the structural characteristics of the biological wing; it still required some design modifications to compare more closely to the biological wing in the flapping motion. Additionally, this research also speaks to the effects of air on the biological wing

Variability in Microphytobenthos Biomass and Carbon Isotopic Values in Shallow Coastal Waters of the Northern Gulf of Mexico

Estuaries and inshore coastal waters of the northern Gulf of Mexico (GOM) are highly productive systems supporting diversity of life, including important fisheries species (e.g., Minello et al. 2003). Salt marshes and seagrass meadows are formed by conspicuous and high-biomass primary producers, long considered important at the base of coastal food webs (Teal 1962). However, the inconspicuous primary producers, phytoplankton and microphytobenthos (MPB, single-celled micro-algae on the sediment surface) are also important in these systems, having been shown to support a variety of consumers (Currin et al. 1995, 2011, Galvan et al. 2008). While disentangling MPB biomass and productivity rates is logistically challenging, there are many studies which suggest both phytoplankton and MPB represent a potentially large portion of primary production in these systems due to the rapid turnover rates (Sullivan and Moncreiff 1988, Blanchard et al. 2002)

MED12 regulates a transcriptional network of calcium-handling genes in the heart

The Mediator complex regulates gene transcription by linking basal transcriptional machinery with DNA-bound transcription factors. The activity of the Mediator complex is mainly controlled by a kinase submodule that is composed of 4 proteins, including MED12. Although ubiquitously expressed, Mediator subunits can differentially regulate gene expression in a tissue-specific manner. Here, we report that MED12 is required for normal cardiac function, such that mice with conditional cardiac-specific deletion of MED12 display progressive dilated cardiomyopathy. Loss of MED12 perturbs expression of calcium-handling genes in the heart, consequently altering calcium cycling in cardiomyocytes and disrupting cardiac electrical activity. We identified transcription factors that regulate expression of calcium-handling genes that are downregulated in the heart in the absence of MED12, and we found that MED12 localizes to transcription factor consensus sequences within calcium-handling genes. We showed that MED12 interacts with one such transcription factor, MEF2, in cardiomyocytes and that MED12 and MEF2 co-occupy promoters of calcium-handling genes. Furthermore, we demonstrated that MED12 enhances MEF2 transcriptional activity and that overexpression of both increases expression of calcium-handling genes in cardiomyocytes. Our data support a role for MED12 as a coordinator of transcription through MEF2 and other transcription factors. We conclude that MED12 is a regulator of a network of calcium-handling genes, consequently mediating contractility in the mammalian heart

Local Structure of Multiferroic TbMn2O5: Evidence for an Anomalous Terbium Oxygen Distribution

The temperature dependent local structure of TbMn2O5 was determined by x-ray absorption spectroscopy. An anomalous Tb-O distribution is found. At high temperature it is broad but resolves into two distinct peaks below approximately 180 K. The distributions sharpen below the Tb magnetic ordering temperature (approximately 10 K). The distortions in the Tb-O distribution, away from the Pbam structure, are consistent with rotations of the MnOx polyhedra about the c-axis and suggest that Tb-O bond polarization may play a significant role in the observed ferroelectric properties of this system.Comment: 7 Figure

20-Hydroxycholecalciferol, Product of Vitamin D3 Hydroxylation by P450scc, Decreases NF-κB Activity by Increasing IκBα Levels in Human Keratinocytes

The side chain of vitamin D3 is hydroxylated in a sequential manner by cytochrome P450scc (CYP11A1) to form 20-hydroxycholecalciferol, which can induce growth arrest and differentiation of both primary and immortalized epidermal keratinocytes. Since nuclear factor-κB (NF-κB) plays a pivotal role in the regulation of cell proliferation, differentiation and apoptosis, we examined the capability of 20-hydroxycholecalciferol to modulate the activity of NF-κB, using 1,25-dihydroxycholecalciferol (calcitriol) as a positive control. 20-hydroxycholecalciferol inhibits the activation of NFκB DNA binding activity as well as NF-κB-driven reporter gene activity in keratinocytes. Also, 20-hydroxycholecalciferol induced significant increases in the mRNA and protein levels of the NF-κB inhibitor protein, IκBα, in a time dependent manner, while no changes in total NF-κB-p65 mRNA or protein levels were observed. Another measure of NF-κB activity, p65 translocation from the cytoplasm into the nucleus was also inhibited in extracts of 20-hydroxycholecalciferol treated keratinocytes. Increased IκBα was concomitantly observed in cytosolic extracts of 20-hydroxycholecalciferol treated keratinocytes, as determined by immunoblotting and immunofluorescent staining. In keratinocytes lacking vitamin D receptor (VDR), 20-hydroxycholecalciferol did not affect IκBα mRNA levels, indicating that it requires VDR for its action on NF-κB activity. Comparison of the effects of calcitrol, hormonally active form of vitamin D3, with 20-hydrocholecalciferol show that both agents have a similar potency in inhibiting NF-κB. Since NF-κB is a major transcription factor for the induction of inflammatory mediators, our findings indicate that 20-hydroxycholecalciferol may be an effective therapeutic agent for inflammatory and hyperproliferative skin diseases

Increasing compliance with wearing a medical device in children with autism

Health professionals often recommend the use of medical devices to assess the health, monitor the well-being, or improve the quality of life of their patients. Children with autism may present challenges in these situations as their sensory peculiarities may increase refusals to wear such devices. To address this issue, we systematically replicated prior research by examining the effects of differential reinforcement of other behavior (DRO) to increase compliance with wearing a heart rate monitor in 2 children with autism. The intervention increased compliance to 100% for both participants when an edible reinforcer was delivered every 90 s. The results indicate that DRO does not require the implementation of extinction to increase compliance with wearing a medical device. More research is needed to examine whether the reinforcement schedule can be further thinned

A Cardiac MicroRNA Governs Systemic Energy Homeostasis by Regulation of MED13

SummaryObesity, type 2 diabetes, and heart failure are associated with aberrant cardiac metabolism. We show that the heart regulates systemic energy homeostasis via MED13, a subunit of the Mediator complex, which controls transcription by thyroid hormone and other nuclear hormone receptors. MED13, in turn, is negatively regulated by a heart-specific microRNA, miR-208a. Cardiac-specific overexpression of MED13 or pharmacologic inhibition of miR-208a in mice confers resistance to high-fat diet-induced obesity and improves systemic insulin sensitivity and glucose tolerance. Conversely, genetic deletion of MED13 specifically in cardiomyocytes enhances obesity in response to high-fat diet and exacerbates metabolic syndrome. The metabolic actions of MED13 result from increased energy expenditure and regulation of numerous genes involved in energy balance in the heart. These findings reveal a role of the heart in systemic metabolic control and point to MED13 and miR-208a as potential therapeutic targets for metabolic disorders.PaperCli

Risperidone-induced weight gain is mediated through shifts in the gut microbiome and suppression of energy expenditure

AbstractRisperidone is a second-generation antipsychotic that causes weight gain. We hypothesized that risperidone-induced shifts in the gut microbiome are mechanistically involved in its metabolic consequences. Wild-type female C57BL/6J mice treated with risperidone (80μg/day) exhibited significant excess weight gain, due to reduced energy expenditure, which correlated with an altered gut microbiome. Fecal transplant from risperidone-treated mice caused a 16% reduction in total resting metabolic rate in naïve recipients, attributable to suppression of non-aerobic metabolism. Risperidone inhibited growth of cultured fecal bacteria grown anaerobically more than those grown aerobically. Finally, transplant of the fecal phage fraction from risperidone-treated mice was sufficient to cause excess weight gain in naïve recipients, again through reduced energy expenditure. Collectively, these data highlight a major role for the gut microbiome in weight gain following chronic use of risperidone, and specifically implicates the modulation of non-aerobic resting metabolism in this mechanism

Thiocillin contributes to the ecological fitness of Bacillus cereus ATCC 14579 during interspecies interactions with Myxococcus xanthus

The soil-dwelling delta-proteobacterium Myxococcus xanthus is a model organism to study predation and competition. M. xanthus preys on a broad range of bacteria mediated by lytic enzymes, exopolysaccharides, Type-IV pilus-based motility, and specialized metabolites. Competition between M. xanthus and prey bacterial strains with various specialized metabolite profiles indicates a range of fitness, suggesting that specialized metabolites contribute to prey survival. To expand our understanding of how specialized metabolites affect predator–prey dynamics, we assessed interspecies interactions between M. xanthus and two strains of Bacillus cereus. While strain ATCC 14579 resisted predation, strain T was found to be highly sensitive to M. xanthus predation. The interaction between B. cereus ATCC 14579 and M. xanthus appears to be competitive, resulting in population loss for both predator and prey. Genome analysis revealed that ATCC 14579 belongs to a clade that possesses the biosynthetic gene cluster for production of thiocillins, whereas B. cereus strain T lacks those genes. Further, purified thiocillin protects B. cereus strains unable to produce this specialized metabolite, strengthening the finding that thiocillin protects against predation and contributes to the ecological fitness of B. cereus ATCC 14579. Lastly, strains that produce thiocillin appear to confer some level of protection to their own antibiotic by encoding an additional copy of the L11 ribosomal protein, a known target for thiopeptides. This work highlights the importance of specialized metabolites affecting predator–prey dynamics in soil microenvironments