Morphological and Biological Characterization of Monosporascus cannonballus isolates, responsible of watermelon decline in Kairouan's area

Watermelon grown in Kairouan's region is infected by vine decline disease caused by M. cannonballus. In vitro tests showed that this pathogen produces perithecia with a diameter of 495 μm releasing ascospores with a diameter of 44 μm. The mycelium is very fine, rarely visible in the media KOMADA and TANAKA. The colony of different isolates on substrate culture such as Malt, MS and S, appears very dense. On PDA, the grayish brown color characteristic of this ascomycete was observed. The perithecia production in vitro test for this fungus requires relatively long period of incubation (45 days). The effect of culture media showed that the PDA, MS and Malt are the best for the mycelia development of M. cannonballus. The most favorable culture media for fructification arein descending order MS, S, PDA, Malt. M. cannonballus isolates tested in this study showed an optimum temperature of mycelial growth and reproduction of 30°C. MT15 andMT12 isolates originated from Chebika (Kairouan government) showed a significantmycelial growth at all temperatures of incubation. MT7 and MT14 isolates from the sameregion were the most fertile. The results of pH effect on M. cannonballus developmenthave revealed that the maximum mycelial growth occurred at pH 6 for most of isolates andfor the fructification occurred at pH 4 and pH 8. For the osmotic pression with the addition of NaCl and KCl, the optimal development for majority of isolates is registeredat -0.5 MPa and -2 MPa, with a minimum at -4MPa. The inhibition of the fructification of the M. cannonballus isolates occurred beyond -2MPa

Screening of fungi implicated in the dieback of olive trees (Olea europea) in Chebika’s area

Several surveys were conducted during spring 2008 in Chebika’s area in Tunisia. Samples were collected from infected plants showed different types of symptoms and they have been the subject of mycological analysis. The morphological identification of fungal colonies isolated from roots, crown and stems of two olive varieties Koroneiki and Chemlali Sfax, revealed the presence of a fungi complex including Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Verticillium dahliae, Cladosporium fulvum, Alternaria solani, Alternaria tenuis, Bispora punctata. and Cylindrocarpon .sp; Although,those fungi Fusarium oxysporum, Fusarium solani, Rhizoctonia solani and Verticillium dahliae are ubiquitous and the predominant one. Pathogenicity results revealed that the fungi isolated from olive trees exhibited typical symptoms on Koroneiki variety incontrolled conditions