61 research outputs found

    Anatomical characteristics of two Ornithogalum L. (Hyacinthaceae) taxa from Serbia and Hungary and their taxonomic implication

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    Anatomical characters of two morphologically similar Ornithogalum taxa, O. umbellatum and O. divergens, were investigated. An analysis of leaf and scapus cross-sections was performed on plants from ten populations from Serbia and Hungary, using light microscopy. The aim of this research was to give data about the qualitative and quantitative anatomical characteristics of these taxa, in order to evaluate their taxonomic significance and single out distinctive anatomical features, as well as to contribute to the knowledge of the genus Ornithogalum in the studied region. On the basis of the variability of anatomical characters, similar populations formed two clusters, joining the plants previously determined as O. divergens and O. umbellatum. The two taxa significantly differed for most of the quantitative leaf and scapus characters. Since only quantitative differences were recorded in this research, anatomical characters could not be solely used to separate these two taxa. However, the results of anatomical investigations are consistent with the results of previous morphological and genetic analyses; therefore anatomical parameters could be useful as additional taxonomic characters

    Structural adaptation of Salsola soda L. (Chenopodiaceae) from inland and maritime saline area

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    The microscopic analysis of leaf and stem in two populations of Salsola soda was carried out in order to examine mechanism of anatomical adaptations to environmental condition on saline habitats and to determine if there exists a morpho-anatomical differentiation between populations from maritime and inland saline area. Analysis included 26 quantitative characters of leaf and stem. The results showed that both populations exhibited halomorphic and xeromorphic adaptations, which refered to ecological plasticity and adaptations of plants to their habitats. Our research also showed that S. soda had quite a stable morphoanatomical structure, since only quantitative changes were recorded. [Projekat Ministarstva nauke Republike Srbije, br. 173002

    Besnilo - epizootioloŔka situacija na teritoriji Srbije i zemalja u okruženju od 2006. do 2012. godine

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    Rabies is a disease that occurs both in animals and people, with relatively long period of incubation, intense clinical course and fatal ending. Majority of cases of illnes and death in people are caused by the standard rabies virus. According to books of regulations on suppression and eradiction of rabies from 1988. and 2009., each suspected rabies has to be laboratory confirmed. From 2006. to 2012. within the passive surveilance, 3549 samples of brain tissue were examined for the presence of rabies virus. The virus was confirmed in 923 samples by using the method of direct imunofluorescence. From 2006. to 2012. there were diagnosed 192, 160, 233, 181, 104, 43 and 10 positive cases, respectively. For the purpose of rabies eradiction at the territory of the Republic of Serbia, a regional project of oral vaccination of foxes against rabies has been implemented since 2010.Within monitoring of the effectiveness of oral vaccination against rabies which was carried out at the end of 2011. and at the beginning of 2012., there were examined 1385 samples, out of which 11 reacted positively. The virus was dominantly present in the fox population. During 2008. and 2009., a relatively large number of rabid cats was registered, what indicates a transmission of rabies from the fox to the cat population. The incidence of rabies at the territory of Serbia significantly differs from one epizootic area to another. Northern parts of the country (Sombor and Subotica epizootic regions) have more favorable situation with only two cases of rabies for the last 7 years. In the period until 2010., in Pozarevac, Novi Sad, Jagodina, Pancevo, Belgrade and Nis epizootic region, a gradual decline in number of positive cases was noticed. International project for oral vaccination of foxes against rabies which has been implemented at the territory of the Republic of Serbia and neighboring countries, influenced a significant reduction of registered rabies cases.Besnilo je bolest životinja i ljudi koja se odlikuje relativno dugim inkubacionim periodom i dramatičnim kliničkim tokom, koji se uvek zavrÅ”ava smrtnim ishodom. Najveći broj slučajeva oboljenja i smrti ljudi potiče od infekcije klasičnim virusom besnila. Prema pravilnicima koji se bave suzbijanjem i iskorenjivanjem besnila iz 1988. i 2009. godine obavezna je laboratorijska potvrda svake klinički opravdane sumnje na besnilo. Od 2006. do 2012. godine u okviru pasivnog nadzora pregledano je 3549 uzoraka moždanog tkiva na prisustvo virusa besnila. Metodom direktne imunofluorescencije virus je dokazan u 923 uzoraka. Po godinama, od 2006. do 2012. godine dijagnostikovano je 192, 160, 233, 181, 104, 43 i 10 pozitivnih slučajeva. U cilju iskorenjivanja besnila na teritoriji Republike Srbije od 2010. godine se sprovodi regionalni projekat oralne vakcinacije lisica protiv besnila. U okviru monitoringa efikasnosti oralne vakcinacije lisica protiv besnila koji je sprovođen krajem 2011. i početkom 2012. godine ispitano je 1385 uzoraka od čega je 11 reagovalo pozitivno. Virus je dominantno prisutan u populaciji lisica. Tokom 2008. i 2009. godine dijagnostikavan je relativno veliki broj besnih mačaka, Å”to ukazuje na prenos virusa iz populacije lisica u populaciju mačaka. Incidencija besnila na teritoriji Srbije se značajno razlikuje od jednog do drugog epizootioloÅ”kog područja. Severni delovi zemlje (somborsko i subotičko epizotioloÅ”ko područje) imaju povoljniju epizootioloÅ”ku situaciju (samo dva slučaja besnila u proteklih 7 godina). U periodu do 2010. godine na požarevačkom, novosadskom, jagodinskom, pančevačkom, beogradskom i niÅ”kom epizootioloÅ”kom području se uočava postepeni pad broja pozitivnih slučajeva. Međunarodni projekat oralne vakcinacije lisica protiv besnila koji se sprovodi na teritoriji Republike Srbije i okolnih zemalja uticao je na to da se broj prijavljenih slučajeva besnila u Srbiji drastično smanji

    Morphogenetic responses of embryo culture of wheat related to environment culture conditions of the explant donor plant

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    Availability of immature embryos as explants to establish wheat (Triticum aestivum L.) by tissue culture can be limited by climatic factors and the lack of high quality embryos frequently hampers experimentation. This study evaluates the effects of rainfall, various temperature-based variables and sunshine duration on tissue culture response (TCR) traits including callus formation (CF), regenerating calli (RC), and number of plants per embryo (PPE) for 96 wheat genotypes of worldwide origin. The objectives of this study were to evaluate the significance of a particular climatic factor on TCR traits and to determine the period of wheat growth during which these factors were the most effective. The genotypes were grown in an experimental field during three seasons differing in meteorological conditions. The relationships between TCR traits and climatic factors within three time periods of wheat growth: 2, 6 and 10 weeks prior to embryo sampling were analysed by biplot analysis. The tissue culture traits were influenced at very different degrees by climatic factors: from 16.8% (RC) to 69.8% (CF). Donor plant environment with high temperatures and low rainfalls reduced (p lt 0.05) the tissue culture performance of wheat genotypes. Callus formation was most sensitive to the temperature based factors. The environmental conditions between flowering and the medium milk stage were the most important for CF, while RC and PPE were not particularly related to any period

    Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace

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    Evaluation of the total antioxidant capacity of solid matrices without extraction steps is a very interesting alternative for food researchers and also for food industries. These methodologies have been denominated QUENCHER from QUick, Easy, New, CHEap, and Reproducible assays. To demonstrate and highlight the validity of QUENCHER (Q) methods, values of Q-method validation were showed for the first time, and they were tested with products of well-known different chemical properties. Furthermore, new QUENCHER assays to measure scavenging capacity against superoxide, hydroxyl, and lipid peroxyl radicals were developed. Calibration models showed good linearity (R2 > 0.995), proportionality and precision (CV < 6.5%), and acceptable detection limits (<20.4 nmol Trolox equiv). The presence of ethanol in the reaction medium gave antioxidant capacity values significantly different from those obtained with water. The dilution of samples with powdered cellulose was discouraged because possible interferences with some of the matrices analyzed may take place.The autonomous government of Castilla y LeoĢn (Project BU268A11-2
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