SPEED UP THE PROCESS OF HEALING THE OPEN WOUNDS BY LOW POWER HE-NE LASER RADIATION

Introduction: Reducing pain and enhancement of wound healing have several advantages. Wound healing is a complicated but regular process that includes synchronized and organized phenomena such as paranchyma cells migration, proliferation and synthesis of extracellular matrix proteins. Rate of wound healing is affected by various factors. The aim of our study was to investigate the effects of low power He-Ne laser radiation (630 nm), on metabolic process, cellular proliferation and finally wound repairment.
 Methods: In our research, about 50 healthy and mature rats were studied by experimental method. After shaving, small cuts (10 mm) were made at the neck. Then they were divided randomly into control and case groups. The rats of the case group were radiated by He-Ne laser in the wounds about 4 minutes each day. After a week, we did wound autopsy of both groups and all the specimens were studied microscopically.
 Results: The findings showed that there were significant increase in the fibroblasts and connective tissue fibers and also vascularisation in the case group compared with the control group.
 Discussion: Wound healing in the case group was accelerated and wound diameter was smaller significantly. So, low power He-Ne laser radiation accelerates wound healing process

The effect of different doses of hyaluronan on sperm morphology, motility, vitality and fertilization capability in mouse

Background: Hyaluronan has an important role on the permeability and motility of sperm and the interaction of gametes and these can play a considerable role on the fertility rate. Therefore, in this study, we assessed the effect of different doses of hyaluronan on the morphology, motility, vitality and fertility rate of mice. Methods: We used 40 mice (6-8 week) in this study which twenty of them were male and the rest were female. The sperm of each male mouse were divided into four groups. The group 1 (control): They were maintained in RPMI media without any hyaluronan supplementation for 2 hour. Hyaluronan with the doses of 750, 1000 and 1250 µg/ml were added into RPMI media in groups 2, 3 and 4, respectively. After 2 hour. incubation, the numbers of sperms were assessed, using haemocytometer. Also, their morphology with papanicolaeu staining and their vitality with Eosin B dye were assessed. As well as sperms motility measured under inverted microscope by observation and fertility rate evaluated after routine IVF by counting two-cell stage embryos. Results: Our results demonstrated that, the dose of 750 µ g/ml has the greatest effect on the motility, vitality and fertility rate of sperms. The effect of dose of 1000 µ g/ml also was positive on them. On the other hand, none of these doses had any effect on sperm morphology. Conclusion: Hyaluronan may have an influence on motility, vitality and fertility rate of sperms and the dose of 750µ g/ml had a significant effect on these factors

Teratogenic effects of caffeine and clomipramine on rat fetus

Background: Obsessive-compulsive disorders and depression have a high prevalence during pregnancy therefore, pregnant women may take clomipramine and also take other drugs or consume foods that contain caffeine. As investigations about the teratogenic effects of clomipramine and its concurrent administration with caffeine during organogenesis period are scarce, we aimed to study the teratogenicity of simultaneous administration of clomipramine and caffeine in rat fetus.Methods: After dividing 42 pregnant rats to several case and control groups, we injected different doses of caffeine and clomipramine to the animals. All the injections were performed on the eighth until the 15th day of pregnancy. We removed the fetuses on the 17th day of pregnancy and studied the morphological features and apparent anomalies of the fetuses macroscopically. Results: We found a significant rate of mortality, apparent anomalies, abnormal torsion, shrinkage of skin and subcutaneous bleeding in fetuses of rats receiving high doses of caffeine or a combination of caffeine and clomipramine. Statistical analysis of the data revealed a significant increase (P?0.001) in teratogenicity of high doses of caffeine and its combination with clomipramine. Conclusion: This study implies simultaneous intake of high amounts of caffeine and clomipramine lead to teratogenicity. We recommend pregnant women to avoid uncontrolled consumption of foods that contain caffeine or drugs that contain high amounts of this substance. They should not also take clomipramine with caffeine in the first trimester of pregnancy

Teratogenic Effects of Co-administration of Fluoxetine and Olanzapine on Rat Fetus

Objective. Prevalence of depression during the pregnancy period is a relatively common problem. Since teratogenic effects of concomitant administration of fluoxetine and olanzapine during the organogenesis period is little known, the aim of the present study was to evaluate the teratogenic effects of a co-administration of fluoxetine and olanzapine on rat fetuses. Method. Forty two pregnant rats were divided into seven groups, randomly. The first group received 0.5 ml of normal saline as the control. The second and third groups received fluoxetine in doses of 9 mg/kg and 18 mg/kg, respectively. Olanzapine was injected at 3 mg/kg and 6 mg/kg to the fourth and fifth groups, respectively. The sixth group received 9 mg/kg fluoxetine and 3 mg/kg olanzapine. Finally, the seventh group was administrated with fluoxetine and olanzapine at 18 mg/kg and 6 mg/kg, respectively. Drugs were injected intraperitoneally between day eight and day 15 of the pregnancy. On the 17th day of pregnancy, the fetuses were removed and micro/macroscopically studied. Results. Fetuses of rats received high doses of these drugs showed a significant rate of cleft palate development, open eyelids and torsion anomalies, comparing to control group (P≤0.001). It is concluded that these drugs can lead to teratogenicity, so their concomitant use during pregnancy should be avoided, or if necessary, their doses must be decreased

The role of clomipramine in potentiating the teratogenic effects of caffeine in pregnant rats: a histopathological study

Since little is known about the teratogenic effects of clomipramine used concurrently with caffeine during the organogenesis period, the aim of this study was to test the teratogenic effects of a coadministration of caffeine and clomipramine on rat fetuses.We divided 42 pregnant rats into seven groups, randomly.The first group (control) received 0.5mL of normal saline.Clomipraminewas injected at 40 mg/kg and 80 mg/kg to the second and third groups, respectively. The fourth and fifth groups received caffeine in doses of 60mg/kg and 120mg/kg, respectively. The sixth group received a combination of 40mg/kg clomipramine and 60mg/kg caffeine, and the seventh group was given clomipramine and caffeine at 80mg/kg and 120 mg/kg, respectively.The fetuses were removed on the 17th day of pregnancy and studied in terms of microscopic and macroscopic morphological features. Fetuses of rats receiving high doses of caffeine or combinations of caffeine and clomipramine showed a significant rate of cleft palate development, open eyelids, mortality, torsion anomalies, shrinkage of skin, and subcutaneous haemorrhage ( ≤ 0.001). This study concludes that caffeine in high doses or the simultaneous administration of caffeine and clomipramine leads to teratogenicity

Effect of red and infrared spectrum low level of laser rays on Rat Seminiferous tubules

Background and Objective: Laser is a source of electromagnetic radiation. Laser therapy has a kind of natural and biological effect on tissue which acts via energy and light power. Todays the use of infrared and red rays from low-power lasers have been established as a routine way for the treatment of diseases. Considering the important role of laser in biological sciences this study was done to compare the effect of red and infrared spectrum low level of laser rays on Rat Seminiferous tubules. Materials and Methods: This experimental study was done on 40 male Rat which divided in four groups including one control and three experimental. In the first experimental group, the right testis of the rats was exposed to a mixture of 300 Hz infra-red ray for 7 minutes and 300 Hz red spectrum for 1 minute daily. In the second experimental group, the right testes were exposed to the 300 Hz infra-red ray for 8 minutes for 40 seconds daily. In the third experimental group, the right testes were exposed to 80 Hz infra-red for 5 minutes and 80 Hz red ray for one minute daily. The controls did not receive any rays. After 15 days, testes were dissected, fixed and stained for histological processing. Thickness of seminiferous tubules and lumen as well as the thickness and area of seminiferous epithelium were measured. The concentration of testosterone was determined with radioimmunoassay. Data was analyzed with SPSS-13 software and ANOVA test. Results: There was a significant difference in the thickness of seminiferous tubules, thickness of lumen space and thickness of epithelium between first (i.e., the mixture of 300 Hz red and infra-red lasers), second (300 Hz infra-red laser) and the third experimental groups (80 Hz red and infra-red lasers) (P<0.05). But no difference was found between the first group and control. The serum testosterone concentration did not show any differences between experimental and control. Conclusion: This study showed that morphologic and morphometric alterations have direct relation with laser energy density

Aloe vera incorporated biomimetic nanofibrous scaffold: A regenerative approach for skin tissue engineering

10.1007/s13726-013-0219-2Iranian Polymer Journal (English Edition)233237-248IPJO