2 research outputs found
Control of MRSA infection and colonisation in an intensive care unit by GeneOhm MRSA assay and culture methods
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major nosocomial
pathogens. Due to the diffusion of MRSA strains in both hospital and community settings,
prevention and control strategies are receiving increased attention. Approximately 25% to 30% of
the population is colonised with S. aureus and 0.2% to 7% with MRSA. The BD GeneOhm MRSA
real-time PCR assay offers quicker identification of MRSA-colonised patients than do culture
methods.
Ninety-five patients admitted to the Intensive Care Unit of IRCCS Policlinico San
Matteo of Pavia (Italy) for a period > 24 h were screened for MRSA colonisation with both the
culture method and the GeneOhm assay.
Of the 246 nasal swabs collected from 95 patients, 36 samples were found to be positive
by both methods (true-positive). 30% of colonised patients had developed the MRSA infection.
Our results show that the GeneOhm MRSA assay is a valuable diagnostic tool for
detecting MRSA quickly in nasal swabs. This study confirms that colonisation represents a high risk
factor for MRSA infection, and that good MRSA surveillance in an Intensive Care Unit is therefore
an excellent way to prevent MRSA infectio