12 research outputs found
Occurrence and phylogenetic analysis of allexiviruses identified on garlic from China, Spain and Poland commercially available on the polish retail market
Serological and molecular detection of bean leaf roll and chickpea chlorotic stunt luteoviruses in chickpea from Iran
Chickpea (Cicer arietinum L.) is an important legume crop and widely cultivated in northwestern
provinces of Iran. During a survey in the 2015 growing season a total of 170 selected
chickpea plants with general yellowing symptoms including stunting and leaf bronzing
were collected. Serological Elisa and tissue blot immunoassay (TIBA) tests revealed
the presence of Bean leaf roll virus (BLRV) and Chickpea chlorotic stunt virus (CpCSV) as
the predominant viruses in the region. Some serologically positive samples of BLRV and
CpCSV were selected and rechecked by RT-PCR. Th e results of amplifi ed PCR products
using a specifi c pair of primers towards the Cp gene region of the viruses were approximately
413 bp for CpCSV and 391 bp for BLRV. Results obtained from sequence comparison
of BLRV (IR-F-Lor-5) isolate form two subgroups with eight other BLRV isolates
from GeneBank indicating a high homology of 96% with isolates from Argentina, Germany,
Tunisia, USA, Spain, and Colombia. An isolate from Norabad (Iran) (IR-Nor) had
98% homology with HQ840727 Libyan isolate. CpCSV sequence comparison with six other
GeneBank isolates indicated 98% homology with isolates from Tunisia and Azerbaijan. Th e
overall results of this research revealed the CpCSV and BLRV (luteoviruses) associated
with the yellowing disease syndrome of chickpea crops in the surveyed region