Chromatography-mass spectrometry studies on the metabolism of synthetic cannabinoids JWH-018 and JWH-073, psychoactive components of smoking mixtures

The lack or low content of their native compounds in urine requires detailed identification of their metabolites, which are excreted with urine and are present in blood. Using gas and liquid chromatography-mass spectrometry, we identified a series of metabolites in urine samples from humans and ratsyesBelgorod State Universit

Long quasi-periodic oscillations of the faculae and pores

Aims. The main goal of this work is to analyze the structural and temporal evolution of small-scale magnetic structures (SSMSs) observed in the solar atmosphere, such as solitary faculae and pores, and reveal long quasi-periodic oscillations of these structures.Methods. The statistical method of regression analysis and the wavelet transform were used to obtain the periods of oscillations and dependences between the parameters of magnetic structures and periods of oscillations.Results. Long-period oscillations with periods in the interval of 18-260 min are found for the structurally stable phase of SSMSs at the level of the solar photosphere. These long-period oscillations were interpreted as natural oscillations of the structurally stable long-lived magnetic structures around their equilibrium position. These oscillations, which are of similar nature, are observed in the chromospheric bright formations associated with photospheric SSMSs. Dependences between the magnetic field and the continuum intensity of the facula elements were found. It is shown that the continuum intensity of a SSMS decreases when its magnetic field increases

Informative significance of serum cytokines and their importance for development of metabolic syndrome with arterial hypertension in elderly persons

Metabolic syndrome (MS) is among the main public health challenges worldwide, leading to significant labor losses, increased costs for treatment and rehabilitation of the patients. The aim of the present study was to identify the informative serum interleukins, by determining the odds ratio in elderly patients with MS and hypertension. The main group of 86 patients with MS and arterial hypertension (AH) aged 60-75 years was examined under clinical conditions. The inclusion criteria were as follows: age of 60-75 years, presence of MS, primary hypertension (grade II-III), absence of acute myocardial infarction, malignant neoplasms, disorders of cerebral circulation, kidney failure over last 6 months. Diagnostics of MS and hypertension was carried out in accordance with Expert Guidelines from the Russian Research Society of Cardiology on the MS Diagnosis and Treatment. Our first study of a large range of serum interleukins in elderly patients with MS and hypertension allowed us to reveal the inversely directed changes in pro- and anti-inflammatory cytokine contents. Combined AH/MS in elderly persons is accomplished by sufficient increase of the most proinflammatory cytokines, and vice versa, by significant decrease in anti-inflammatory cytokines in blood serum. This finding clearly points to importance of immunological regulatory systems for initiation of AH with MS at older age. Pro- and anti-inflammatory serum interleukins are actively involved into the AH/MS development in elderly accompanied by their pronounced imbalance. The mentioned immune reactions could underlie the MS/AH condition. High risk of this disorder is connected with changed production of proinflammatory cytokines (IL-8, IL-1β), like as anti-inflammatory serum interleukins (IL-4, IL-10), with predominance of the former. The above interleukins should be considered dominant diagnostic markers of AH/MS in elderly persons. Measurement of serum interleukins and discriminant-based approach allows highly reliable differentiation of elderly patients with AH/MS from similar individuals without this disorder

Differential diagnostics of the syndrome of the dyspepsia of the infectious and noninfectious genesis

Timely and correct differential diagnostics of dyspeptic syndrome was and remains serious interdisciplinary problem. The greatest complexities appear with the development of the reasons for the development of diarrhea and ab-dominal pain in patients with the komorbidnoy pathology. Understanding the mechanisms, which lie at the basis of the formation of syndrome of diarrhea, they will make it possible within maximally short times to manufacture adequate diagnostic tactics and to determine the necessary therapeutic measures. In the article the typical defects of diagnostics of the diseases, which are accompanied by the syndrome of diarrhea, are analyzed, in the prehospital and hospital stages of rendering to medical aid and they are proposed to the way of their warning and correction.Серьезной междисциплинарной проблемой была и остается своевременная и правильная дифференциальная диагностика диспепсического синдрома. Наибольшие сложности возникают при выявлении причин развития диареи и абдоминальной боли у больных с коморбидной патологией. Понимание механизмов, лежащих в основе формирования диарейного син-дрома, позволят в максимально короткие сроки выработать адекватную диагностическую тактику и определить необходимые лечебные мероприятия. В статье проанализированы типичные дефекты диагностики заболеваний, сопровождающихся синдромом диареи, на догоспитальном и госпитальном этапах оказания медицинской помощи и предложены пути их предупреждения и исправления

Flu in children: Clinical, laboratory indicators and cytokine profile parameters

Introduction. Respiratory diseases occupy a leading place in the structure of pathology of childhood. The proportion of influenza and acute respiratory viral infections among all infectious diseases is 90 %. The literature describes gender differences in the immune response to respiratory infections in children, but there is a gap in  the  description of the cytokine profile in  children with influenza depending on gender and age.The aim. To analyze clinical and laboratory parameters as well as cytokine profile parameters in children with influenza.Materials and methods. A single-stage descriptive study was conducted with the participation of 50 children from 1 to 11 years of age with a diagnosis of influenza who were on inpatient treatment at the Irkutsk Regional Infectious Diseases Clinical Hospital from December 2018 to January 2019. The clinical and laboratory features of the course of influenza in children, the duration of treatment were determined. The concentration of cytokines interleukin (IL)  1β, IL-4, IL-6, IL-8, tumor necrosis factor alpha (TNF-α), interferon alpha and gamma (INF-α, INF-γ) in blood plasma was determined by enzyme-linked immunosorbent assay (ELISA) using diagnostic test systems manufactured by Vector-Best (Novosibirsk, Russian Federation) on the analyzer Multiscan EX (Thermo Electron, Germany).The control group consisted of practically healthy children without signs of acute respiratory viral infection (n = 50; mean age 5.3 ± 2.6 years).Results. When comparing clinical and laboratory data and cytokine profile parameters in children with influenza, no gender differences were found. There was a statistically significant increase in the level of pro-inflammatory cytokines IL-1β, IL-6, IL-8, TNF-α, INF-α, as well as CRP, anti-inflammatory cytokine IL-4 in influenza in all age categories, in contrast to the control group (p < 0.05).Conclusion. Influenza in children of different sexes proceeds classically without a statistical difference in clinical and laboratory parameters and in the level of cytokines

State of the intestinal microbiota in patients with Covid-19

The purpose of the study is to clarify the state of the intestinal microbiota in a new coronavirus infection.Цель исследования — уточнить состояние микробиоты кишечника при новой коронавирусной инфекции

Intestinal damage in patients with new coronavirus infection (Covid–19) due to connective tissue dysplasia

The aim of the study - to evaluate the intestinal damage in patients with COVID–19 on the background of CTDЦель исследования – оценить поражение кишечника у пациентов с COVID–19 на фоне ДС