Optimization of Gallic Acid Production from Terminalia Chebula by Aspergillus niger

A method for producing gallic acid by microbiological hydrolysis of the tannins of myrobalan seed powder is described in the present work. Hydrolysis of gallotannins of the substrate to gallic acid by Aspergillus niger MTCC 282 was studied. A simple extraction procedure is used. Fungal mycelia pre-induced with 5 g/L gallotannin was used as inoculums. Optimal conditions of production were determined using various parameters including gallotannin concentration, nutritional source and metal ions are determined. Gallotannin is hydrolyzed with acid, and gallic acid in the hydrolyses is then assayed using rhodanine. This method is very specific: no interferences from other plant phenolics, including ellagic acid and condensed tannin, have been observed. The yield of gallic acid with respect to gallotannins present in the substrate is estimated. Yields of gallic acid are about 74% with respect to gallotannin concentration, which suggests that this method is exploitable industrially for the manufacturing trimethoprim drug

OPTIMIZATION OF ENVIRONMENTAL PARAMETERS FOR MAXIMUM TANNASE PRODUCTION FROM CASHEW HUSK

Tannase production under solid-state fermentation was investigated using isolated Aspergillus oryzae. Among all agro-industrial waste material evaluated, cashew husk supported maximum tannase production. The metabolic processes of microorganisms are influenced by changes in parameters like Temperature, pH, incubation time, humidity etc., which are very specific for a particular organism. Microbial synthesis of enzymes in a SSF process are also affected by factors like particle size of substrate, water content, relative humidity, type and size of inoculum, control of temperature, period of cultivation, etc. Biotransformation of cashew husk tannin to gallic acid by SSF is also influenced by all the factors affecting tannase production, since the synthesized enzyme causes the breakdown of tannin to gallic acid and glucose

Tannase Production by Aspergillus niger

A method for assay of microbial tannase (Tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Maximum Tannase production occurred in the culture broth containing 1-2% (w/v) tannic acid and 0.05 – 0.1% (w/v) glucose. The pH, incubation period, temperature and Glucose concentration optima of Tannase production was found at 5.5, 36 h, 35°C and 0.5% respectively. These properties make the enzyme suitable for pollution control and bioprocess industry. This assay is very simple, reproducible, and very convenient, and with it Tannase activity can be measured in relation to the growth of the organism. Aspergillus niger exhibited higher enzyme activity showing about 65 mole percent conversion respectively after a 36 h incubation period. The assay is complete in a short time, very convenient and reproducible

Optimization of Protease Production from Aspergillus Oryzae Sp. Using Box-Behnken Experimental Design

Protease production by Aspergillus oryzae was optimized in shake-flask cultures using Box-Behnken experimental design. An empirical model was developed through response surface methodology to describe the relationship between tested variable (peptone, glucose, soyabeanmeal and pH). Maximum enzyme activity was attained with Peptone at 4 g∕L; temperature at 30 °C glucose at 6 g∕L; 30 °C and pH at 10. Experimental verification of the model showed a validation of 95%, which is more than 3-fold increase compare to the basal medium