Effect of varying levels of bypass fat on digestibilities of nutrients and balances of N, P and Ca in lactating Jaffrabadi buffaloes

Lactating Jaffrabadi buffaloes (24; first to fourth lactation and average body weight 650.0 kg) were randomly divided into 4 groups of 6 animals each as per completely randomized design and allocated to 4 dietary treatments as T1,T2, T3 and T4. Experimental buffaloes were offered commercial concentrate mixture and cotton seed cake in equal proportion (50:50) to fulfil the protein requirements as per ICAR (1998) along with the basal diet of 10 kg seasonal green and mature pasture grass hay ad lib. Commercial bypass fat was provided to all experimental buffaloes at the rate of 150g per animal for first 15 days prior to parturition and at the rate of 0, 10g, 20g and 30g /kg milk yield in T1, T2, T3 and T4 groups, respectively post partum. DMI, Per cent DMI and DMI/kg W0.75 of lactating buffaloes remained non significant among different treatment groups during entire experiment. Lactating buffaloes also showed non-significant differences in body weight and biometry during different phases of experiment. Differences in water intake (l/day, l/100kg B.W., l/kg W0.75 and l/kg DMI) by different levels of bypass fat supplementation were also non significant. Digestibility of the rations for DM, OM, CP, EE, CF and NFE were not significantly affected by addition of supplemental fat. Nitrogen, phosphorus and calcium balances were positive and non significant among different treatment groups. Plane of nutrition as per ICAR nutritional requirements for Indian buffaloes was comparable among different dietary treatments. Overall results indicates that bypass fat supplementation has positive but non significant effect on nutrient utilization in different groups

Indigenous Lactobacillus strains improve growth performance and high density cholesterol levels in broilers

Antibiotics have been used extensively in poultry chicken to promote growth rate, increase feed conversion efficiency, and prevent intestinal infections, resulting in an imbalance of the beneficial intestinal flora. The use of lactic acid bacteria as feed additives to substitute antibiotic associated growth stimulators as well as their impact on meat quality, could possibly be the major approach. In this context, here, we studied two Lactobacillus cultures viz., L. plantarum KGL3A and L. fermentum KGL4 as an alternative to growth promoters. Broilers were grouped into four different treatments: T1 (control: basal diet + antibiotic growth promoter and immunomodulatory factor), T2 [basal diet without having antibiotic growth promoter and immunomodulatory factor + L. plantarum KGL3A (108 CFU/mL)], T3 [basal diet without having antibiotic growth promoter and immunomodulatory factor + L. fermentum KGL4 (108 CFU/mL)] and T4 (basal diet without having antibiotic growth promoter and immunomodulatory factor + combination of T3 and T4 bacterial strains). During the entire study, higher bodyweight was observed among the Lactobacillus fed broilers groups (T4: 2433g, T3: 2371 g, T2: 2355 g) as compared to the control group (T1: 2339 g). Lipid profile analysis further confirmed the significant decrease in low-density lipoprotein (LDL) content of T4 (19%) and T3 (16%) groups than the control group while more than 10% increase in high-density lipoprotein HDL content was observed in T4 and T3 groups than the control group. Further, the decrease in coliform and enterococci counts and an increase in Lactobacillus counts in treatment groups compared to the control group were found. The results indicate that the potential use of Lactobacillus cultures (KGL3A and KGL4) as dietary feed supplements as alternative to the antibiotics as growth promoters in poultry feeds

IN VITRO ANTIBIOTIC SENSITIVITY PATTERN OF Brucella spp. ISOLATED FROM REPRODUCTIVE DISORDERS OF ANIMALS

ABSTRACT Out of 248 samples processed, a total 10 Brucella could recover, three from cows, two from a buffaloes, four from goats and one from a bitch by cultural, morphological, biochemical characteristics and PCR methods. Among the 20 antibiotics tested against the 10 Brucella isolates, variable sensitivity was observed. All the isolates were 100% sensitive to penicillin-G, streptomycin, gentamicin, choramphenicol, erythromycin, kanamycin, ciprofl oxacin, tetracycline, oxytetracycline, doxycycline hydrocloride, amikacin and enrofl oxacin. Whereas 80% of the isolates found sensitive to tobramycin, 70% to ampicillin/ salbactum, 60% to rifampicin, 50% to methicillin and 40% of the isolates found sensitive to cefriaxone. While cefuroxime and vancomycin were found only 20% sensitive and ampicillin / cloxacillin was found 100% resistant to Brucella isolates. Thus the present fi nding a could be useful to the clinician and veterinary practitioner to prevent the further progression of disease and further development of complications in infected human patients and animals by selecting appropriate antibiotics

Comparison of Rose Bengal Plate Agglutination, Standard tube agglutination and Indirect ELISA tests for detection of Brucella antibodies in Cows and Buffaloes

A total of 180 serum samples (107 cows, 73 buffaloes) from cases of abortion and various reproductive disorders were collected for detection of Brucella antibody by Rose Bengal Plate Agglutination Test (RBPT), Serum Tube Agglutination Test (STAT) and indirect- ELISA (i-ELISA). The overall prevalence of brucellosis by RBPT, STAT and i-ELISA were 11.21%, 16.00% and 24.30% in cows 9.59%, 12.33% and 26.03% in buffaloes respectively. Overall seroprevalence of Brucellosis in cases of abortion, R.O.P. by RBPT, STAT and i-ELISA were 11.32%, 16.04% and 32.08% respectively. When three serological tests were compared, seropositivity was found highest by i-ELISA (25%), followed by STAT (14.45%) and RBPT (10.56%). The results shows higher prevalence of brucellosis in cases of abortion and R.O.P., while at lower level from various reproductive disorders as detected serologically indicating endemicity of the infection in villages around Anand city, Gujarat. [Vet. World 2010; 3(2.000): 61-64