4 research outputs found
A novel polyclonal antibody-based sandwich ELISA for detection of Plasmodium vivaxdeveloped from two lactate dehydrogenase protein segments
Role of Azithromycin against Clinical Isolates of Family Enterobacteriaceae: A Comparison of its Minimum Inhibitory Concentration by Three Different Methods
Purpose: To determine the effect of azithromycin, a new azalide
antibiotic, on clinical isolates of the family Enterobacteriaceae and
to determine and compare its minimum inhibitory concentration (MIC) by
disk diffusion, agar dilution and E-test methods. Materials and
Methods: One hundred fifty-nine bacterial strains belonging to the
family Enterobacteriaceae, isolated from different clinical samples,
were tested for their susceptibility to azithromycin by disk diffusion,
agar dilution and E-test methods. The MIC values were analysed and the
percentages of agreement between the different methods were mentioned.
Results: Of the 159 isolates of the family Enterobacteriaceae, 60.37%
were E. coli followed by Klebsiella species 28.3%, Salmonella and
Shigella species 3.77% and Enterobacter and Citrobacter species 1.88%
each. Maximum isolates were obtained from urine 117/159 (73.58%).
Azithromycin was found to be more active against Salmonella and
Shigella species, showing 100% sensitivity the by E-test and 83.33% by
the disk diffusion methods. In the agar dilution method, 83.33% of
Salmonella and 66.66% of Shigella species were sensitive to
azithromycin. The overall agreement between disk diffusion and agar
dilution method was 96.8%, between agar dilution and E-test was 88% and
between disk diffusion and E-test was 91.2%. Conclusion: Azithromycin
may become an important addition to our antimicrobial strategies,
especially for the treatment of bacterial diarrhoea and infections
caused by Salmonella typhi