Biochem-Env, une plateforme de biochimie environnementale au service de la recherche

La plateforme Biochem-Env est une plateforme technique qui permet le développement et la veille technologique sur les outils de caractérisation biochimique (activités enzymatiques globales, phospholipidiques, activité catabolique...) de matrices environnementales solides (sols et sédiments), ainsi que sur les outils de caractérisation biochimique de la macrofaune terrestre et benthique (biomarqueurs enzymatiques, réserves énergétiques...). Elle ambitionne notamment d’améliorer les outils biochimiques existants pour l’évaluation de la qualité biologique des milieux et de proposer à la normalisation AFNOR et ISO de nouveaux protocoles expérimentaux. La plateforme Biochem-Env se dote actuellement de moyens analytiques permettant d’augmenter ses capacités d’analyse de biomarqueurs enzymatiques et lipidiques. Elle intervient ainsi dans le suivi de sites expérimentaux de longue durée (Systèmes d’observation et d’expérimentation pour la recherche en environnement - SOERE), et des projets de recherches visant à mettre en évidence l’impact de polluants sur le fonctionnement biologique des sols et des organismes du so

Biochem-Env, a plateform of environmental biochemistry for research

As a service of the research infrastructure AnaEE-France (http://www.anaee-france.fr/fr/), the platform Biochem-Env (http://www.biochemenv.fr) offers skills and innovative analytical tools for biochemical characterizations of soils, sediments, and micro-macro-organisms living in terrestrial and aquatic ecosystems. The platform provides methods validated according to Quality Guidelines, i.e. to measure global soil enzymatic activities. Our robot-supported protocols allow great number of enzyme measurements (hydrolase and oxidase activities) with high reproducibility. It has been involved in programs targeted at assessing the sensitivity and restrictions of biological indicators of soil quality, subjected to various constraints (waste landfilling, farming practices, contaminated sites and soils…). The platform is also at the origin of an ISO standard (ISO_TS_22939). Available as a scientific partner in the frame of collaborative research projects, the platform’s abilities ranges from advice providing (sampling, protocols and data analysis), to technical training, including analysis and experimentation in regional, national and international research programs

Development of a soft extraction method for sulfamethoxazole and transformation products from agricultural soils: Effects of organic matter co-extraction on the environmental availability assessment

The recycling of biosolids and livestock manure in agriculture may lead to the introduction of antibiotic residues, i.e., parent molecule and transformation products, into amended soils. Their fate in soils can be approached through the assessment of their environmental availability. In this work, the environmental availability of sulfamethoxazole (SMX) and three transformation products (N4-acetyl-SMX, 3-amino-5-methylisoxazole, aniline) was assessed in soils amended with sludge compost or cow manure throughout a three-month incubation, using soft extractions with CaCl2, EDTA or cyclodextrin solutions. First, the freeze-storage of soil samples was shown to decrease the SMX extractability. The SMX extractability depended on the initial concentration, the amendment type and the extracting solution at day 0. From 1.9% up to 63% of the SMX total content was initially extractable. The lowest fractions were quantified in EDTA extracts in which the dissolved organic matter was the most complex and responsible for high matrix effects in mass spectrometry compared to CaCl2 extracts. The purification of cyclodextrin extracts highly reduced the matrix effects, but CaCl2 was considered as the most suitable extractant. SMX extractability strongly decreased after the first 8days of incubation to finally reach 0.4-0.8% after 84days, whatever the initial conditions. This high decrease could be related to humification observed through the increasing complexity of extracted dissolved organic matter. Very low levels of transformation products were quantified throughout the incubation period. The low environmental availability of SMX was mainly due to its sorption on soil organic matter and resulted in its low biotransformation in these amended soils