Phenotypic data

Phenotypic data at the Ester resistance to insecticide locus in the Cx pipiens mosquito from the Montpellier (south of France) over the 1986-2012 period. Site is the location of the sampled population, Km is the distance in kilometers between the population and the Mediteranean Sea, n the number of individuals phenotyped in each sampled population a given year and [i] the number of individuals presenting phenotype i

Insecticide quantity data

The total amount of organophosphate insecticides applied (T) each year by the local agency for mosquito control in the treated area are presented. From 1986 to 1989, treatment applications (i.e., the size of the treated area and the amounts used) did not differ significantly from those in 1990. We therefore attributed the same amount of OPs to the years for which this information was missing (italics)

Activity, preimaginal mortality and resistance data

Original data of activity, preimaginal mortality and resistance of homozygotes for single copies and two duplicated alleles of the ace-1 gene and their hybrids. For each variable the indiduals data are provided

Costs and benefits of multiple resistance to insecticides for mosquitoes-1

Ur strains of mosquito.<p><b>Copyright information:</b></p><p>Taken from "Costs and benefits of multiple resistance to insecticides for mosquitoes"</p><p>http://www.biomedcentral.com/1471-2148/8/104</p><p>BMC Evolutionary Biology 2008;8():104-104.</p><p>Published online 8 Apr 2008</p><p>PMCID:PMC2359736.</p><p></p

Costs and benefits of multiple resistance to insecticides for mosquitoes-0

Nt. Insecticide susceptible strain SLAB is shown in an open column and the three resistant strains are shown in hatched columns. In two replicate experiments, there were nine or ten pots initially containing 100 larvae for each of the four strains. Different letters above columns indicate significant differences (< 0.05) in female emergence as found by a Tukey-Kramer test (see text for details).<p><b>Copyright information:</b></p><p>Taken from "Costs and benefits of multiple resistance to insecticides for mosquitoes"</p><p>http://www.biomedcentral.com/1471-2148/8/104</p><p>BMC Evolutionary Biology 2008;8():104-104.</p><p>Published online 8 Apr 2008</p><p>PMCID:PMC2359736.</p><p></p

Costs and benefits of multiple resistance to insecticides for mosquitoes-2

Ticide when applied alone. Open symbols are for the amount of each insecticide required for the same effect when the two are mixed together at a ratio based on their median-effect doses (± 95% confidence intervals). Points above the matching line indicate antagonistic activity of the insecticides, close to the line indicates additivity of action, and those below the line indicate synergistic activity.<p><b>Copyright information:</b></p><p>Taken from "Costs and benefits of multiple resistance to insecticides for mosquitoes"</p><p>http://www.biomedcentral.com/1471-2148/8/104</p><p>BMC Evolutionary Biology 2008;8():104-104.</p><p>Published online 8 Apr 2008</p><p>PMCID:PMC2359736.</p><p></p

Infection status of wild caught <i>Ae. albopictus</i> as determined with standard PCR [11].

<p>A−B+ and A+B+ refer to <i>w</i>AlbB singly infected and <i>w</i>AlbA/<i>w</i>AlbB co-infected mosquitoes, respectively. Differences between male and female infection patterns were analysed using Fisher's exact test (<i>p</i>-values are reported for each sample, in italics when significant).</p

Mean <i>Wolbachia</i> density in wild specimens from La Réunion Island.

<p>Each DNA was quantified in triplicate and the average density was calculated for each specimen. Mean density in the population is reported for each sex and is depicted in black for <i>w</i>AlbA and grey for <i>w</i>AlbB. Standard error is calculated on the mean of all average densities (males N = 15, females N = 10).</p

Crossing relationships of <i>Culex pipiens</i> isofemale lines according to <i>w</i>Pip groups.

<p>Total indicates the total number of reciprocal crosses performed to established CI patterns, and N the number of crosses that were compatible (C), uni-directionally incompatible (UIC) and bi-directionally incompatible (BIC). SD = standard deviation. In incompatible crosses, HR  = 0%; in compatible crosses, HR >90%. For more details about crosses within <i>w</i>Pip groups see Tables S2, S3, S4, S5 and S6 in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087336#pone.0087336.s001" target="_blank">File S1</a> whilst for crosses between <i>w</i>Pip groups see Tables S7, S8, S9 and S10 in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087336#pone.0087336.s001" target="_blank">File S1</a>.</p

Biochemical and larvicidal properties of the most potent PTFs.

a<p>numbers and groups refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047125#pone.0047125.s003" target="_blank">Table S1</a>.</p>b<p>IC₅₀ values were determined from regression analysis of log-concentrations versus percentage inhibitions. R_IC50 =  IC₅₀ WT/IC₅₀ G119S. Compounds were sorted by their R_IC50 ratio.</p>c<p>Mortality was measured from bioassays on Slab (OP-sensitive) and SR (OP-insensitive) strains exposed for 24 hours to 300 µM PTF. R_m300 =  SR mortality/Slab mortality.</p>d<p>PTFs biochemically characterized are in bold.</p>e<p>Hit compound from the primary screen.</p>f<p>Compounds with R_IC50 and R_m300 above 1.5.</p