2 research outputs found

    ANTI-CANCER ACTIVITY OF DATURA METEL IN MCF-7 CELL LINE

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    Objective: Current clinical trends involve the usage of plants as therapeutic agent in a wide range of applications. Present investigation is focused on the anticancer activity of the methanol extract of Datura metel against MCF-7 cell line.Materials & Methods: The study was facilitated by collecting the plant sample and subjected to methanol extraction using Soxhlet apparatus.Results: The anticancer activity of the extracted sample against MCF-7 cell line was examined by MTT assay.Conclusion: The study confirms that the leaf extract of Datura metel has anticancer potential against MCF-7 cell lines. Key words: Datura Metel, MCF-7 cell line, MTT assay, anti-cancer activit

    Transplantation of human neural progenitor cells secreting GDNF into the spinal cord of patients with ALS: a phase 1/2a trial.

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    Amyotrophic lateral sclerosis (ALS) involves progressive motor neuron loss, leading to paralysis and death typically within 3-5 years of diagnosis. Dysfunctional astrocytes may contribute to disease and glial cell line-derived neurotrophic factor (GDNF) can be protective. Here we show that human neural progenitor cells transduced with GDNF (CNS10-NPC-GDNF) differentiated to astrocytes protected spinal motor neurons and were safe in animal models. CNS10-NPC-GDNF were transplanted unilaterally into the lumbar spinal cord of 18 ALS participants in a phase 1/2a study (NCT02943850). The primary endpoint of safety at 1 year was met, with no negative effect of the transplant on motor function in the treated leg compared with the untreated leg. Tissue analysis of 13 participants who died of disease progression showed graft survival and GDNF production. Benign neuromas near delivery sites were common incidental findings at post-mortem. This study shows that one administration of engineered neural progenitors can provide new support cells and GDNF delivery to the ALS patient spinal cord for up to 42 months post-transplantation
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