MOLECULAR SEXING PADA ELANG JAWA (Nisaetus bartelsi Stresemann, 1924) dan ELANG BRONTOK (Nisaetus cirrhatus Gmelin, 1788) HASIL SITAAN BKSDA DI YOGYAKARTA

Penentuan jenis kelamin pada burung Elang Jawa (Nisaetus bartelsi Stresemann, 1924) dan Elang Brontok (Nisaetus cirrhatus Gmelin, 1788) sangat penting dalam program konservasi dan pembiakan untuk meningkatkan populasi di habitatnya. Penentuan jenis kelamin sangat sulit karena burung ini termasuk burung monomorfik, sehingga perlu pendekatan molekuler untuk menentukan jenis kelamin. Tujuan penelitian ini untuk menentukan jenis kelamin Elang Jawa dan Elang Brontok yang berasal dari hasil sitaan Balai Konservasi Sumberdaya Alam (BKSDA) di Kota Yogyakarta dengan metode molecular sexing, mengetahui primer yang paling efektif untuk mengidentifikasi jenis kelamin dan karakterisasi sekuen gen CHD-W dan gen CHD-Z. Penelitian ini menggunakan teknik Polymerase Chain Reaction (PCR) dengan Taq polimerase Direct Animal dan Hot Start. Primer yang digunakan sejumlah empat pasang, yaitu primer P2/P8, primer 2550F/2718R, primer 1237L/1272H, dan primer 2561/2728, yang mengamplifikasi gen CHD (chromo helicase DNA binding). Sampel DNA diperoleh dari 6 individu, yaitu dua sampel Elang Jawa, dan empat sampel Elang Brontok. Berdasarkan penelitian yang dilakukan diperoleh hasil bahwa molecular sexing dapat digunakan untuk mengidentifikasi jenis kelamin Elang Jawa dan Elang Brontok berdasarkan gen CHD dengan hasil empat sampel berupa individu betina dan dua sampel berupa individu jantan. Primer yang paling efektif untuk mengidentifikasi jenis kelamin adalah pasangan primer 2550F/2718R dan primer 2561/2728 yang mengamplifikasi 100% sampel ditunjukkan dengan adanya pita DNA tunggal (ZZ) pada jantan dan adanya pita DNA ganda (ZW) pada betina. Ukuran gen CHD menggunakan primer 2550F/2718R memiliki ukuran DNA pada kromosom CHD-Z dengan panjang 372 – 490 bp, dan CHD-W dengan panjang 640 – 758 bp serta ukuran gen CHD menggunakan primer 2550F/2718R memiliki ukuran DNA pada kromosom CHDZ dengan panjang 638 – 725 bp dan CHD-W dengan panjang 444 – 450 bp

Rural men and mental health: their experiences and how they managed

There is a growing awareness that a primary source of information about mental health lies with the consumers. This article reports on a study that interviewed rural men with the aim of exploring their mental health experiences within a rural environment. The results of the interviews are a number of stories of resilience and survival that highlight not only the importance of exploring the individuals' perspective of their issues, but also of acknowledging and drawing on their inner strengths. Rural men face a number of challenges that not only increase the risk of mental illness but also decrease the likelihood of them seeking and/or finding professional support. These men's stories, while different from each other, have a common thread of coping. Despite some support from family and friends participants also acknowledged that seeking out professional support could have made the recovery phase easier. Mental health nurses need to be aware, not only of the barrier to professional support but also of the significant resilience that individuals have and how it can be utilised

Ageism Within Feminism

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/98225/1/j.1547-5069.1993.tb00781.x.pd

The scenes and spaces of anxiety: Embodied expressions of distress in public and private fora

Psychological treatments of mental health issues have acquired a justifiable notoriety for their tendency to engage in generalisation and reductionism. By contrast, the emergent geographies of exclusion make visible the fine-grain material and spatial contours of the lives of individuals who experience mental health difficulties and distress. However, this can come at the cost of a relative neglect of the psychological. In this paper we propose a set of concepts for facilitating the study of intersecting planes of experience, which demonstrates the interdependency of the spatial, the psychological and the technological. Drawing on empirical work with participants who live with persistent anxiety, we demonstrate how online support networks mediate – that is transduct, intersect and transform – how experiences of anxiety are lived out. Attention to endogenous ‘tactics’ or ‘modes of normativity’ provides an interesting agenda for the emergent engagement of social psychology with social/cultural geography

Making A Difference: Psychology And The Construction Of Gender

https://works.swarthmore.edu/alum-books/3460/thumbnail.jp