21 research outputs found
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Not AvailableSequence-related amplified polymorphism (SRAP) was used to assess the genetic diversity of 114 pomegranate accessions sourced from all over the world and maintained in the field gene bank at ICAR-Indian Institute of Horticultural Research, Hesaraghatta, Bangalore. Totally 10 SRAP combinations were used for the study. Genetic parameters such as effective alleles (Ne), Nei genetic diversity (H), Shannon index (I), and polymorphic information content (PIC) were calculated based on molecular data. The number of alleles per locus ranged from 1.66 to 4.00 with an average of 2.03. The average polymorphic information content (PIC) value was 0.24. The expected heterozygosity varied from 0.215 to 0.445 with an average of 0.306. Cluster analysis was performed using R software. All the 114 accessions were grouped into four major clusters. There was no clear grouping based on origin. The analysis of molecular variance (AMOVA) indicated insignificant genetic variation (p=0.31) between pomegranate accessions from different geographical locations. Overall genetic variation among the population groups was low, while 99% of variation was due to within group differences. These results confirmed that SRAP markers could be a powerful and an effective marker system for determining the genetic diversity and population genetic structure of the pomegranate.ICAR-IIH
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Not AvailableA study was undertaken to investigate the possible effects of different chemicals (Nitrobenzene (NB) @ 1.0 ml
plant-1, NB @ 1.5 ml plant-1, NB @ 2.0 ml plant-1, Cycocel (CCC) @ 500 ppm plant-1, CCC @ 1000 ppm plant-1, CCC @
1500 ppm plant-1, Uracil @ 25 ppm plant-1, Uracil @ 50 ppm plant-1, CCC @ 1000 ppm plant-1 + Uracil @ 25 ppm plant-
1, CCC @ 1500 ppm plant-1+ Uracil @ 50 ppm plant-1) on fruiting, fruit quality characteristics concomitant with
anthocyanin content in pomegranate cv Bhagwa during the years 2016 – 17 at ICAR – IIHR, Hesaraghatta. The results
showed that the fruits with highest weight (197.55 g), length (6.64 cm), total aril weight (113.63 g), percentage of aril
weight (62.11 %), 100 aril weight (27.18 g), juice weight (101.94 g) and percentage (51.19 %) accompanied with highest
anthocyanin content (6.647 mg 100g-1) were obtained with application of CCC @ 1000 ppm plant-1 in combination with
Uracil @ 25 ppm. Highest fruit diameter (6.90 cm), TSS content (19.96 0B), TSS to acid ratio (46.68) and less titrable
acidity (0.41 %) were apparent with foliar spray of Cycocel @ 1500 ppm plant-1, while its application @ 1000 ppm has
resulted in highest fruit volume (162.70 ml).Not Availabl
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Not AvailablePomegranate is quite popular among consumer for its striking, sweet acidic taste and
refreshing arils. Pomegranate is one of the ancient fruit crops which were originated in
Iran. Total 151 pomegranate genotypes along with their hybrid parents (Bhagwa, DF,
Nana) combinely 154 used for morphological characterization. Total 13 morphological
parameters were used for characterization and Analysis of Variance, mean comparison and
simple correlations were analysed. Significance differences (P<0.05) were found among
all the genotypes of pomegranate. Highest critical differences at 5% were 13.21 (Fruit
weight), 11.98 (Aril %), 2.552Thorn [No/Shoot length (m)] recorded and lowest in leaf
blade width 0.143, 0.145 in leaf blade length, 0.456 in fruit length and 0.48 in seed
hardiness respectively. Plant growth parameters, leaf parameters and fruit parameters are
significantly correlated to each other. Fruit weight and seed hardiness was negatively
correlated to each other (r= -0.42). Those fruit have larger size and weight have less seed
hardiness. Fruit characteristics are negatively correlated with leaf characteristics (r= -
0.22).The largest the leaf size smallest the fruit size.Not Availabl
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Not AvailableTo realize the productivity potential of pomegranate cv. Bhagwa, studies were conducted
on the effects of chemicals Nitrobenzene (1.0, 1.5 and 2.0 ml plant-1), Cycocel (500, 1000
and 1500 ppm plant-1) and Uracil (25 and 50 ppm plant-1) on flowering behavior and fruit
yield during the years 2016-17. Highest number of hermaphrodite flowers (264.66), fruit
yield (27.10 kg tree-1 and 18.86 t ha-1) and fruit number tree-1 (154) were realized with
application of uracil @ 50 ppm. However, the uracil treatment did not influence
significantly the number of male and intermediate flowers. Application of Cycocel @ 1000
ppm in combination with Uracil @ 25 ppm plant-1 has recorded highest fruit weight
(197.55g). At flowering stage, the gas exchange parameters like the photosynthetic rate
(16.48 μmol m-2 s-1) was higher in plants treated with Uracil @ 50 ppm while transpiration
rate was higher (7.72 mmol m-2 s-1) with uracil @ 25 ppm. Higher stomatal conductance
(0.44 mol m-2 s-1) was recorded under nitrobenzene @ 1.5ml plant-1 treatment. During fruit
set stage, photosynthetic rate was high (12.55 μmol m-2 s-1) in plants treated with
nitrobenzene @ 1.5 ml plant-1 whereas, transpiration rate (6.19 mmol m-2 s-1) and stomatal
conductance (0.18 mol m-2 s-1) were higher in plants treated with Cycocel @ 1000 ppm +
Uracil @ 25 ppm plant-1. Thus Uracil @ 50 ppm alone seems to be effective in enhancing
fruit yield and the beneficial effects were associated following increases in gas exchange
parameters and bisexual flowers.Not Availabl
TDZ-induced direct shoot organogenesis and somatic embryogenesis on cotyledonary node explants of lentil (Lens culinaris Medik.)
An efficient and simple procedure for inducing high frequency direct shoot organogenesis and somatic embryogenesis in lentil from cotyledonary node explants (without both the cotyledons) in response to TDZ alone is reported. TDZ at concentration lower than 2.0 μM induced shoot organogenesis whereas at higher concentration (2.5–15 μM) it caused a shift in regeneration from shoot organogenesis to somatic embryogenesis. The cotyledonary node and seedling cultures developed only shoots even at high concentrations of BAP and TDZ, respectively. TDZ at 0.5 and 5.0 μM was found to be optimal for inducing an average of 4–5 shoots per cotyledonary node in 93 % of the cultures and 55 somatic embryos in 68 % of the cultures, respectively. The somatic embryos were germinated when transferred to lower TDZ concentration (0.5–1.0 μM). The shoots were rooted on MS basal medium containing 2.5 μM IBA. The plantlets were obtained within 8 weeks from initiation of culture and were morphologically similar to seed-raised plants. The possible role of stress in thidiazuron induced somatic embryogenesis is discussed