Lead biosorption by a bacterium isolated from industrial effluents

An attempt was made to isolate microorganisms from the industrial effluents and to study their role in bioremediation of lead in selected industrial effluents. Sixty bacterial strains from the industrial effluent were isolated, purified, preliminarily identified and examd. for their ability to uptake lead. Based on the min. inhibition concns. (MICs) of lead for the selected isolates, six strains were found to be most resistant isolates and amongst them one isolate showed max. resistance to lead which was identified as Bacillus cereus. Scanning electron microscopic (SEM) photographs and Energy dispersive X-​ray spectroscopy (EDS) signature of Bacillus cereus revealed that lead was adsorbed to the cell surface, confirming biosorption capacity of the bacteria

Lead biosorption by resting cells of Bacillus cereus

A study on the lead biosorption by resting cells of lead resistant bacteria, isolated from industrial effluents, was carried out to ascertain their biosorption capacities. The strain showing highest MIC (min. inhibition concn.) for lead was selected for the study and identified as Bacillus cereus. Lead biosorption studies on Bacillus cereus pretreated with phys. (heat and oven dried) and chem. (sodium azide) methods showed improved lead biosorption with the exception of heat treatment in comparison to live biomass. Among the pretreatment methods, azide treatment showed max. lead biosorption.

Biosorption of lead by Bacillus cereus isolated from industrial effluents

Aims: To study the biosorption of lead ions from soln. using Bacillus cereus isolated from industrial effluents collected from Peenya industrial area, Bangalore, India and to det. the optimum conditions for biosorption. Study Design: Exptl. study. Place and Duration of Study: Department of Microbiol. and Biotechnol., Bangalore University, Bangalore, Karnataka, India, between Oct. 2008 and Dec. 2009. Methodol.: Sixty bacterial strains were isolated from industrial effluents collected from Peenya industrial area. Among the sixty isolates only six were selected for further investigation due to their high min. inhibition concn. for lead. Lead biosorption studies were carried out for all the six isolates using at. absorption spectrometry. The optimum conditions (temp., pH and culture age) for biosorption were detd. for the isolate showing highest lead biosorption. Results: The lead biosorption capability of all six isolates was studied at different concns. of lead (100, 200, 300, 400 and 500 mg​/l)​. The isolate 6 showed highest lead biosorption capability and was identified as Bacillus cereus. Studies on the control of environmental factors revealed that an optimum temp. of 30°C and pH 5, facilitates max. biosorption of lead by 24h old culture of Bacillus cereus. Conclusion: Biosorption is an alternative to traditional physicochem. methods for removing toxic metals from wastewaters. The results of this study are discussed in the light of the biosorption capacity of Bacillus cereus that could be exploited in the bioremediation of lead

Effect of lead on metallothionein concentration in lead-resistant bacteria Bacillus cereus isolated from industrial effluent

Metallothioneins (MTs) are cysteine-rich metal-binding proteins found in a wide variety of organisms including bacteria, fungi as well as all eukaryotic plant and animal species. MTs bind essential and nonessential heavy metals. MTs production was evaluated by a simple spectrophotometry methodology. The study elucidated that the metallothionein concentration in Bacillus cereus treated with different concentrations of lead increased with increasing lead concentration. The protein samples from B.cereus treated with different concentrations of lead were isolated and electrophoresed on SDS-PAGE and 2-D gels. The study of gels revealed that a thick protein band appeared around 14.3KD in case of B. cereus treated with lead unlike the control samples. © 2011 Academic Journals

Effect of Lead on Growth, Protein and Biosorption Capacity of Bacillus Cereus Isolated from Industrial Effluen

A bacterial strain (Bacillus cereus) with the ability to grow under conditions of high concentrations of lead was isolated from the industrial effluent collected from Peenya Industrial Area, Bangalore. The effect of lead on growth, protein content and lead biosorption capacity of Bacillus cereus was investigated. The results revealed that with increase in lead concentration (100, 200, 300, 400 and 500 mg l-1) there was a decrease in growth, protein content (10.6, 8.2, 6.7, 3.8 and 1.9 mg g-1 d. wt.) and lead biosorption ( 90.3, 57.8, 48.94, 31.3 and 22.24 %) Bacillus cereus, signifying toxic effect of lead on the bacterial strain. Plasmid DNA was isolated from Bacillus cereus to study its resistance mechanism. The size of the plasmid was approximately 33kb. Transformation results suggest that lead resistance gene may be present on the chromosomal DNA rather than the plasmid DNA as the transformants did not show lead resistanc

Evaluation of pre-jamming indication parameter during blind backfilling technique

AbstractHydraulic blind backfilling is used to reduce subsidence problems above old underground water-logged coal mines. This paper describes experimental research on a fully transparent model of a straight underground mine gallery. An automatic data acquisition system was installed in the model to continuously record the sand and water flowrates along with the inlet pressure of the slurry near the model's inlet. Pressure signature graphs and pressure loss curves with bed advancement under different flow conditions are examined. Pressure signature analyses for various flowrates and sand slurry concentrations are conducted to evaluate a pre-jamming indication parameter, which could be used to indicate the arrival of the final stage of filling

Polyvinyl Acetate Resin as a Binder Effecting Mechanical and CombustionProperties of Combustible Cartridge Case Formulations

Several combustible cartridge case (CCC) formulations containing nitrocellulose (NC) as anenergetic ingredient with diphenylamine (DPA) as stabiliser, cellulose fiber as reinforcing materialand polyvinyl acetate (PVAc) as a binder are processed to form combustible discs. A formulationcontaining  nitrocellulose (83 parts), cellulose (17 parts) , PVAc (20 parts) and  diphenylamine(1 part) by weight was observed with the improvement of  mechanical properties without anyadverse effect on combustion characteristics, safety parameters and compatibility with triplebase multi-tubular propellant. Dynamic firing performance was also satisfactory

D-branes in Unoriented Non-critical Strings and Duality in SO(N) and Sp(N) Gauge Theories

We exhibit exact conformal field theory descriptions of SO(N) and Sp(N) pairs of Seiberg-dual gauge theories within string theory. The N=1 gauge theories with flavour are realized as low energy limits of the worldvolume theories on D-branes in unoriented non-critical superstring backgrounds. These unoriented backgrounds are obtained by constructing exact crosscap states in the SL(2,R)/U(1) coset conformal field theory using the modular bootstrap method. Seiberg duality is understood by studying the behaviour of the boundary and crosscap states under monodromy in the closed string parameter space.Comment: 23 pages, 2 figure