23 research outputs found
Microbiologia do meato m茅dio na rinossinusite cr么nica Microbiology of middle meatus in chronic rhinosinusitis
Este foi um estudo prospectivo que visou identificar a microbiologia do meato m茅dio em pacientes com rinossinusite cr么nica (RSC) e compar谩-la com a de indiv铆duos sadios. MATERIAL E M脡TODOS: Foram inclu铆dos 134 pacientes RSC e 50 volunt谩rios sadios, que constitu铆ram o grupo controle. As amostras foram coletadas endoscopicamente e submetidas a exames pelo m茅todo de Gram com contagem leucocit谩ria e culturas para aer贸bios, anaer贸bios e fungos. RESULTADOS: Nos pacientes com RSC foram cultivados 220 microorganismos, dentre os quais os mais freq眉entes foram o Staphylococcus aureus, presente em 31% das amostras, e o Staphylococcus coagulase-negativo (SCN) em 23%. Gram-negativos ou facultativos foram isolados em 37% das amostras, anaer贸bios em 12%, e fungos em 14%. Ao exame bacteriosc贸pico evidenciou-se alguns ou numerosos leuc贸citos em 74% das amostras com culturas positivas. Nos indiv铆duos sadios o SCN foi isolado em 40% das amostras e o Staphylococcus aureus em 18%. Em 12% dos indiv铆duos a cultura para fungos foi positiva, e o exame direto negativo. Todas as culturas anaer贸bias foram est茅reis. Quanto 脿 contagem leucocit谩ria todos apresentaram nenhum ou raros leuc贸citos. CONCLUS脙O: Os grupos apresentaram resultados semelhantes quanto 脿 microbiologia, entretanto, diferiram em rela莽茫o 脿 contagem leucocit谩ria, o que auxilia na diferencia莽茫o um microorganismo infectante de um colonizante.<br>This was a prospective study which assessed endoscopically collected middle meatus secretions in patients with chronic rhinosinusitis (CRS) and compared those findings with microbiological data of healthy individuals. METHODS: Middle meatus samples were collected from 134 CRS patients. In the laboratory, samples were Gram stained for microscopic examination with white blood cels (WBCs) count and also send for aerobic, anaerobic and fungal cultures. Fifty volunteers served as control. RESULTS: In CRS patients a total of 220 microorganisms were isolated. The most frequent microorganisms were Staphylococcus aureus (31%), coagulase-negative Staphylococcus (CNS) (23%). Gram-negative or facultative microorganisms were isolated in 37% of the samples, anaerobes in 12% and fungi in 14%. Seventy four percent of the samples with positive cultures presented many or few WBC. In the control group, 76% of cultures were positive for aerobes and 12% for fungi. No anaerobes were isolated. There were rare or no WBC in the fifty samples. The most frequent microorganisms were CNS (40%), Staphylococcus aureus (18%). CONCLUSION: The microbiology of the middle meatus is similar in CRS patients and healthy individuals. Despite this, there was an important difference between the WBC count in these two groups, which helps to distinguish an infective from a saprophitic microorganism