An analysis of depot level repairables carcass management and position controls under the Advanced Traceability and Control (ATAC) program

Repairables are components andor sub assemblies which can be repaired if they become unserviceable. Repairables are typically high cost, long procurement leadtime items. Because of these characteristics, significant economies can be achieved by repairing these items rather than discarding them when they become unserviceable. Historically, these carcasses have been difficult to manage because, when they broke, maintenance personnel were primarily concerned with replacing them with ready-for-issue units; what happened to the carcass was of little concern. Defense Management Review Decision (DMRD) 901's objective to reduce supply system costs includes an initiative to achieve savings by retaining retrograde carcasses returned from the fleet at the first turn-in point rather than shipping them immediately to the repair depot or designated storage site. This thesis analyzes the operation of the ATAC Program to determine a 'ship or hold' decision for returned carcasses. A thorough study of ATAC's background, current management controls and operating procedures, and results from previous studies were combined with on-site hub observations to show how and why the ATAC system works. Because of ATAC, the DMRD 901 initiative to retain carcasses at their first turn-in point is not cost effective except for those items experiencing rapid phase-out or numerous upgrades. Detailed indicators to measure and monitor ATAC cost and performance effectiveness need to be implemented.http://archive.org/details/annalysisofdepot1094527594Lieutenant Commander, United States NavyLieutenant, United States NavyApproved for public release; distribution is unlimited

Recovery of bisulfite-converted genomic sequences in the methylation-sensitive QPCR

Many methods for the detection of genomic DNA methylation states have appeared. Currently, nearly all such methods employ bisulfite-mediated deamination of denatured DNA. While this treatment effectively deaminates cytosines to uracils, leaving most 5-methylcytosines intact, it also introduces abasic sites that generate a significant number of single-strand breaks in DNA. We have investigated the interplay of these two processes in order to determine their relative effects on the methylation-sensitive QPCR method. The extent of cleavage of the input DNA is significant and appears to be an increasing function of DNA concentration. Even so, the results suggest that only ∼10% of a 62-nt target will be lost due to degradation and targets up to 131 nt will suffer only a 20% loss. More significant losses were found to occur during the subsequent removal of bisulfite and desulfonation steps that appear to be the result of size selectivity associated with matrix binding and elution required prior to QPCR in the most commonly used protocols. For biospecimens yielding <1 μg of DNA, these findings suggest that bisulfite treatment, in current implementations of MS-QPCR, result in low recoveries that preclude reliable analysis of DNA methylation patterns regardless of target size

Heart Rate Variability : Effect of Exercise Intensity on Postexercise Response

The purpose of the present study was to investigate the influence of two exercise intensities (moderate and severe) on heart rate variability (HRV) response in 16 runners 1 hr prior to (-1 hr) and at +1 hr, +24 hr, +48 hr, and +72 hr following each exercise session. Time domain indexes and a high frequency component showed a significant decrease (p < .001) between -1 hr and +1 hr for severe intensity. The low frequency component in normalized units significantly increased (p <.01) for severe intensity at +1 hr. Only severe exercise elicited a change in HRV outcomes postexercise, resulting in a reduction in the parasympathetic influence on the heart at +1 hr; however, values returned to baseline levels by +24 hr

Hamster and Murine Models of Severe Destructive Lyme Arthritis

Arthritis is a frequent complication of infection in humans with Borrelia burgdorferi. Weeks to months following the onset of Lyme borreliosis, a histopathological reaction characteristic of synovitis including bone, joint, muscle, or tendon pain may occur. A subpopulation of patients may progress to a chronic, debilitating arthritis months to years after infection which has been classified as severe destructive Lyme arthritis. This arthritis involves focal bone erosion and destruction of articular cartilage. Hamsters and mice are animal models that have been utilized to study articular manifestations of Lyme borreliosis. Infection of immunocompetent LSH hamsters or C3H mice results in a transient synovitis. However, severe destructive Lyme arthritis can be induced by infecting irradiated hamsters or mice and immunocompetent Borrelia-vaccinated hamsters, mice, and interferon-gamma- (IFN-γ-) deficient mice with viable B. burgdorferi. The hamster model of severe destructive Lyme arthritis facilitates easy assessment of Lyme borreliosis vaccine preparations for deleterious effects while murine models of severe destructive Lyme arthritis allow for investigation of mechanisms of immunopathology

Bark Beetle and Wood Borer Infestation in the Greater Yellowstone Area During Four Postfire Years

Extensive surveys of bark beetles and wood bores in the Greater Yellowstone area were conducted in 1991 through 1993. The study objectives were to determine the effect of delayed tree mortality following the 1988 fires on mosaics of fire-killed and green tree stands, the relationship between fire injury and subsequent infestation, and the effect of insect buildup in fire injured trees on infestation rates for uninjured trees. Surveys were conducted adjacent to roads, and plots wee selected randomly. In 1991, 321 plots were measured, 198 plots in 1992, and 127 plots in 1993. Insects killed 12.6 percent of the Douglas-fir, 17.9 percent of the lodgepole pine, 6.6 percent of the Engelmann spruce, 7.5 percent of the subalpine fire, and 2.8 percent of the whitebark pine. Delayed mortality attributed to fire injury accounted for more mortality than insects. Both types of mortality greatly altered the original fire-killed/green tree mosaics that were apparent immediately after the 1988 fires. Insect infestation was strongly and positively correlated with the percent of the basal circumference of the tree that was fire killed in the species, except in Engelmann spruce where infestation peaked in the middle fire-injury class. Infestation in Douglas-fir, lodgepole pine, and Engelmann spruce increased through 1992 then declined in 1993. Although it cannot be said with certainty that insects built up in fire-injured trees and then caused increased infestation of uninjured trees, the high level of infestation suggests this is the case

Transgene-induced CCWGG methylation does not alter CG methylation patterning in human kidney cells

Several reports suggest that C(m)CWGG methylation tends not to co-exist with (m)CG methylation in human cells. We have asked whether or not methylation at CCWGG sites can influence CG methylation. DNA from cells expressing an M.EcoRII–GFP fusion was actively methylated at CCWGG sites. CG methylation as measured by R.HpaII/R.MspI ratios was unchanged in cells expressing the transgene. Cloned representatives of C(m)CWGG methylated DNA often contained, or were adjacent to an ALU repeat, suggesting that M.EcoRII-GFP actively methylated gene-rich R-band DNA. The transgenic methyltransferase applied C(m)CWGG methylation to a representative human promoter that was heavily methylated at CG dinucleotides (the SERPINB5 promoter) and to a representative promoter that was essentially unmethylated at CG dinucleotides (the APC promoter). In each case, the CG methylation pattern remained in its original state, unchanged by the presence of neighboring C(m)CWGG sites. Q-PCR measurements showed that RNA expression from the APC gene was not significantly altered by the presence of C(m)CWGG in its promoter. Kinetic studies suggested that an adjacent C(m)CWGG methylation site influences neither the maintenance nor the de novo methylation activities of purified human Dnmt1. We conclude that C(m)CWGG methylation does not exert a significant effect on CG methylation in human kidney cells