On the size and shape of excluded volume polymers confined between parallel plates

A number of recent experiments have provided detailed observations of the configurations of long DNA strands under nano-to-micrometer sized confinement. We therefore revisit the problem of an excluded volume polymer chain confined between two parallel plates with varying plate separation. We show that the non-monotonic behavior of the overall size of the chain as a function of plate-separation, seen in computer simulations and reproduced by earlier theories, can already be predicted on the basis of scaling arguments. However, the behavior of the size in a plane parallel to the plates, a quantity observed in recent experiments, is predicted to be monotonic, in contrast to the experimental findings. We analyze this problem in depth with a mean-field approach that maps the confined polymer onto an anisotropic Gaussian chain, which allows the size of the polymer to be determined separately in the confined and unconfined directions. The theory allows the analytical construction of a smooth cross-over between the small plate-separation de Gennes regime and the large plate-separation Flory regime. The results show good agreement with Langevin dynamics simulations, and confirm the scaling predictions.Comment: 15 pages, 3 figure

Early responses to chemotherapy detected by pulse cytophotometry.

DNA/cell distributions were recorded by automated cytofluorometry (=pulse cytophotometry) in bone-marrow aspirates of leukaemia and lymphosarcoma patients subjected to chemotherapy. In most cases, early perturbations in DNA/cell histographs were observed, characteristically reflecting the known mode of action of the drugs. These changes in general preceded the clinical observation of drug response. In a series of 23 measurements in 19 patients, a positive correlation between early cytophotometric changes and clinical effects of chemotherapy was observed in 17 patients. Five patients were negative for both cytophotometric and clinical reactions and one patient was probably false-positive. The validity of the assay for early detection of drug resistance in acute leukaemia and related diseases is discussed

Axiomatic Characterization of the Mean Function on Trees

A mean of a sequence π = (x1, x2, . . . , xk) of elements of a finite metric space (X, d) is an element x for which is minimum. The function Mean whose domain is the set of all finite sequences on X and is defined by Mean(π) = { x | x is a mean of π } is called the mean function on X. In this paper the mean function on finite trees is characterized axiomatically

The Effect of the Outer Lindblad Resonance of the Galactic Bar on the Local Stellar Velocity Distribution

Hydro-dynamical modeling of the inner Galaxy suggest that the radius of the outer Lindblad resonance (OLR) of the Galactic bar lies in the vicinity of the Sun. How does this resonance affect the distribution function in the outer parts of a barred disk, and can we identify any effect of the resonance in the velocity distribution f(v) actually observed in the solar neighborhood? To answer these questions, detailed simulations of f(v) in the outer parts of an exponential stellar disks with nearly flat rotation curves and a rotating central bar have been performed. For a model resembling the old stellar disk, the OLR causes a distinct feature in f(v) over a significant fraction of the outer disk. For positions <2kpc outside the OLR radius and at bar angles of \~10-70 degrees, f(v) inhibits a bi-modality between the low-velocity stars moving like the local standard of rest (LSR) and a secondary mode of stars predominantly moving outward and rotating more slowly than the LSR. Such a bi-modality is indeed present in f(v) inferred from the Hipparcos data for late-type stars in the solar neighborhood. If one interpretes this observed bi-modality as induced by the OLR -- and there are hardly any viable alternatives -- then one is forced to deduce that the OLR radius is slightly smaller than Ro. Moreover, by a quantitative comparison of the observed with the simulated distributions one finds that the pattern speed of the bar is 1.85+/-0.15 times the local circular frequency, where the error is dominated by the uncertainty in bar angle and local circular speed. Also other, less prominent but still significant, features in the observed f(v) resemble properties of the simulated velocity distributions, in particular a ripple caused by orbits trapped in the outer 1:1 resonance.Comment: 14 pages, 10 figures (Fig.2 in full resolution available upon request), accepted for publication in A

Faculty and student perceptions of the feasibility of individual student–faculty meetings

The extent to which students feel involved in their education positively influences academic achievement. Individual student–faculty meetings can foster student involvement. To be effective, faculty acknowledgement of the benefit of these meetings is a prerequisite. The aim of this study was to explore faculty perceptions of individual student–faculty meetings. In addition we investigated students’ perceptions. As part of the undergraduate programme, mandatory individual intake and follow-up meetings between first-year medical students (n = 425) and senior faculty members (n = 34) have been implemented from 2009 onwards. We administered a questionnaire on faculty perceptions of the benefit and impact of intake meetings. Subsequently, after both meetings had been held, strong and weak points of the mandatory programme were explored using open-ended questions. Students’ perceptions were investigated by open-ended questions as a part of the curriculum evaluation process. Faculty enjoyed the meetings (90 %), perceived the meetings to be beneficial (74 %) and expected a positive effect on student involvement (74 %). Faculty appreciated the opportunity to give advice tailored to students’ personal needs and levels of performance. The students appreciated the meetings and the attention given to their personal situation and study progress. Faculty and student appreciation of the meetings seems to support the assumption that the individual meetings increase students’ social and academic involvement. Further research should focus on the impact of individual student–faculty meetings on students’ learning behaviours