Effect of channel width on human umbilical vein endothelial cell (HUVEC) culture in microfluidic channels

This report describes the development of endothelial cell (EC) cultivation devices with different channel widths (60 to 360 μm). Crucial features of the devices include even cell distribution along the channel, seeding reproducibility, and compatibility with microscopy and flow application. The main achievement of this work is the design of chips which allow reproducible HUVEC culture in narrow (&lt; 400 μm) channels.</p

Characterization of a hydrostatically driven cell seeding procedure using polymer microspheres

This paper describes how particles are distributed upon introduction into microchannels using a seeding technique based on hydrostatic flow. The distribution of particles and/or cells in microchannels is characterized in time, and an approach for ensuring a reproducible distribution which involves closing off reservoirs at defined times is presented.</p