Differential regulation of Ota and Otb, two primary glycine betaine transporters in the methanogenic archaeon Methanosarcina mazei go1

Methanogenic archaea accumulate glycine betaine in response to hypersalinity, but the regulation of proteins involved, their mechanism of activation and regulation of the corresponding genes are largely unknown. Methanosarcina mazei differs from most other methanoarchaea in having two gene clusters both encoding a potential glycine betaine transporter, Ota and Otb. Western blot as well as quantitative real-time PCR revealed that Otb is not regulated by osmolarity. On the other hand, cellular levels of Ota increased with increasing salt concentrations. A maximum was reached at 300-500 m M NaCl. Ota concentrations reached a maximum 4 h after an osmotic upshock. Hyperosmolarity also caused an increase in cellular Ota concentrations. In addition to osmolarity Ota expression was regulated by the growth phase. Expression of Ota as well as transport of betaine was downregulated in the presence of glycine betaine. Copyright (c) 2007 S. Karger AG, Basel

Classifying LEP Data with Support Vector Algorithms

We have studied the application of different classification algorithms in the analysis of simulated high energy physics data. Whereas Neural Network algorithms have become a standard tool for data analysis, the performance of other classifiers such as Support Vector Machines has not yet been tested in this environment. We chose two different problems to compare the performance of a Support Vector Machine and a Neural Net trained with back-propagation: tagging events of the type e+e- -> ccbar and the identification of muons produced in multihadronic e+e- annihilation events.Comment: 7 pages, 4 figures, submitted to proceedings of AIHENP99, Crete, April 199

Quantum Tunneling and Phase Transitions in Spin Systems with an Applied Magnetic Field

Transitions from classical to quantum behaviour in a spin system with two degenerate ground states separated by twin energy barriers which are asymmetric due to an applied magnetic field are investigated. It is shown that these transitions can be interpreted as first- or second-order phase transitions depending on the anisotropy and magnetic parameters defining the system in an effective Lagrangian description.Comment: 18 pages, 7 figure

Instructions on reasonable doubt: defining the standard of proof and the juror's task

[see journal for abstract] summary provided here: In the present paper, we measure the effectiveness of two specific instructions on RD that have been used and challenged in the US. These are described in more detail below. We examine the effect of the two instructions on people’s quantitative interpretations of the standard, as well as the degree of inter- and intra-individual variability in interpretations. In addition, we attempt to explain the effectiveness of these instructions by examining the effect of their precise wording on interpretations of RD

Effects of judicial instructions and juror characteristics on interpretations of beyond reasonable doubt

Purpose and Methods: The standard of proof, beyond reasonable doubt (BRD), serves as a threshold for reaching verdicts in criminal cases. Past research has demonstrated that factors such as the wording of judicial instructions defining the standard can influence people’s interpretation of it. In addition, there is some concern that instructions may not be effective for the wider jury-eligible population. In an experimental study involving members of the general public, we examined the effect of two commonly used judicial instructions (i.e., sure and firmly convinced) against a situation when BRD was undefined, on people’s quantitative interpretations of BRD as well as on their self-reported understanding of the standard and confidence in applying it. We also explored the effect of juror characteristics (i.e., gender, age and education). Results: Compared to when the standard was undefined, the sure instruction helped to reduce inter-individual variability in interpretations of BRD and the firmly convinced instruction increased people’s understanding of the standard. However, neither instruction was effective in increasing confidence in applying the standard or in reducing observed individual differences. Conclusion: These findings underscore the importance of developing evidence-based judicial instructions that can benefit the broad jury-eligible population equally and in a variety of ways

A re-examination of the acquittal biasing effect of offence seriousness

The justice system should operate free of any bias, and jurors’ judgments of a defendant’s guilt should be based on evidential factors alone. However, research suggests that this does not always occur. We aimed to investigate the biasing effect of offence seriousness, a case-related, extra-legal factor, on juror decision-making. In an experiment, we examined the effect of this extra-legal factor on 118 members of the jury eligible publics’ interpretations of Beyond Reasonable Doubt (BRD), probability of commission, verdict, and confidence in verdict. We found that defendants charged with more serious offences were judged to be less likely to have committed the crime. However, offence seriousness did not have a significant effect on interpretations of BRD or verdict. The present findings suggest a need to instruct jurors on the application of legal (probative) factors alone

Evaluation of a New Recombinant K39 Rapid Diagnostic Test for Sudanese Visceral Leishmaniasis.

A new rK39 rapid diagnostic dipstick test (DiaMed-IT-Leish) was compared with aspiration and a direct agglutination test (DAT) for diagnosis of visceral leishmaniasis (VL) in 201 parasitologically confirmed cases, 133 endemic controls, and in 356 clinical suspects in disease-endemic and -epidemic areas in Sudan. The sensitivity of the rK39 test in parasitologically confirmed VL cases was 90%, whereas the specificity in disease-endemic controls was 99%. The sensitivity of the DAT was 98%. In clinically suspected cases, the sensitivity of the rK39 test was 81% and the specificity was 97%. When compared with the diagnostic protocol based on the DAT and aspiration used by Médecins sans Frontières in epidemic situations, the positive predictive value was 98%, and the negative predictive value was 71%. This rK39 rapid diagnostic test is suitable for screening as well as diagnosis of VL. Further diagnostic work-up of dipstick-negative patients with clinically suspected VL is important. The ease and convenience of the dipstick test will allow decentralization and improved access to care in disease-endemic areas in Sudan