Zastosowanie manualnych technik osteopatycznych w leczeniu bólu pleców u kobiet w ciąży

Changes in body posture, musculoskeletal disorders and somatic dysfunctions are frequently observed during pregnancy, especially ligament, joint and myofascial impairment. The aim of the paper is to present the use of osteopathic manipulative treatment (OMT) for back and pelvic pain in pregnancy on the basis of a review of the available literature. MEDLINE and Cochrane Library were searched in January 2014 for relevant reports, randomized controlled trials, clinical and case studies of OMT use in pregnant women. Each eligible source was verified and analyzed by two independent reviewers. OMT procedures appear to be effective and safe for pelvic and spinal pain management in the lumbosacral area in pregnant women.W trakcie ciąży u kobiet zachodzą zmiany w postawie ciała, układzie mięśniowo-szkieletowym oraz zaburzenia somatyczne. Najczęściej dochodzi do dysfunkcji więzadeł, stawów i powięzi. Celem pracy jest przedstawienie wykorzystania bezpiecznych osteopatycznych technik manualnych w leczeniu bólu pleców i miednicy u kobiet w ciąży, na podstawie przeglądu dostępnej literatury naukowej. Przegląd literatury przeprowadzono w bazach danych MEDLINE i Cochrane Library w styczniu 2014 roku. Do analizy wybrano prace poglądowe, randomizowane, kliniczne oraz studium przypadku dotyczące terapii manualnej u kobiet w ciąży. Każda praca była weryfikowana i analizowana przez dwóch niezależnych badaczy. Osteopatyczne techniki manualne (OMT) wydają się być skuteczną i bezpieczną terapią w bólach odcinka lędźwiowo-krzyżowego kręgosłupa i miednicy u kobiet w ciąży

The influence of soybean extract on the expression level of selected drug transporters, transcription factors and cytochrome P450 genes encoding phase I drug-metabolizing enzymes

Objective: Soybean phytoestrogens, such as genistein and daidzein, reduce climacteric symptoms and the risk of certain chronic diseases such as cancer and cardiovascular diseases. Despite their widespread use in functional foods and dietary supplements, there is very little data available on their safety and herb-drug interactions, especially with antineoplastic agents. Hence, the aim of our study was to assess the effects of soybean extracts on the expression level of CYP genes and their transcriptional factors. We also investigated the effect of soybean on the mRNA level of transporters, such as P-glycoprotein (MDR1) and multidrug resistance-associated proteins (MRP1, MRP2). Materials and methods: Wistar rats were fed a standardized soybean extract (100 mg/kg, p.o.). cDNA was synthesized from total RNA isolated from different tissues (liver and intestinal epithelium) using reverse transcription. Gene expression level was analyzed by RT-PCR method. Results: We demonstrated a significant increase of CYP1A1 mRNA level (by 89%, p=0.002 and 125%, p=0.004) as compared with the control group. An increase of AHR and CAR expression after 10 days was also observed (by 60%, p0.05, respectively). Additionally, an inductive effect for CYP2D1 by 22% (p=0.008), Mdr1a by 267% (

The -323P0/P10 factor VII gene polymorphism and the risk of recurrent miscarriage

Objectives: Genetically determined disturbances in the activity of coagulation factor VII may lead to obstetric complications. The aim of the study was to evaluate the correlation between -323P0/P10 factor VII gene polymorphism and the risk of recurrent miscarriage. Material and methods: The study group consisted of 152 women with a history of ≥2 miscarriages. The control group comprised 180 women with no history of miscarriage and ≥1 pregnancy who gave birth to a healthy newborn at term. The study group was further subdivided twice into two subgroups: 114 patients with a history of 2 miscarriages and 38 subjects with a history of ≥3 miscarriages, and 123 patients with miscarriage

Effect of Epilobium angustifolium and Serenoa repens extracts on regulation of non-genomic signaling pathway of kinases

Objectives: Changes of kinase activity of non-genomic cellular signaling pathway may influence the effectiveness of pharmacotherapy in case of hormone-dependent tumors. Our study investigated a possible interaction at the molecular level between an aqueous herbal extract of Epilobium angustifolium as well as a lipid-sterolic fruit extract of Serenoa repens and synthetic drugs used in the treatment of hormone-dependent cancers. Material and methods: E. angustifolium and Serenoa repens extracts were orally administered to testosteroneinduced rats for 21 days. Changes of RafA/Mapk3/Mapk1 mRNA levels were analyzed by real-time quantitative PCR using target specific primers. Results: The level of RafA mRNA slightly increased in rats receiving Epilobium angustifolium (p=0.076) and Serenoa repens (p=0.016) extracts. Administration of these extracts resulted in significantly elevated Mapk1 and Mapk3 transcripts in the investigated animals (

Inherited thrombophilia as the reason of recurrent miscarriges in the first trimester of pregnancy

Summary Introduction: It is currently believed that disturbances of maternal clothing system leading to occurrence of thrombotic abnormalities, conditioned by the presence of acquired or inherited thrombophilias, may be an important reason for recurrent abortions. The aim of this study was to investigate frequency and significance of polymorphisms in genes coding for factor V (1691G>A) and factor II (20210G>A) of coagulation cascade in a group of women with two or more miscarriages in the first trimester of pregnancy. Material and methods: The investigations were conducted in a group of 104 women with anamese history of two or more miscarriages in the first trimester of pregnancy and in a group of 169 women with correct obstetrical anamnesis and confirmed presence of at least one pregnancy that resulted in a birth of a healthy child. The analysis was performed by polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP). Results: Investigation of 20210G>A polymorphism of prothrombin gene revealed high overrepresentation of genotype GA (8.33% and 1,18% in the control group, p=0.07) and allele A (4.17% and 0,59% in the control group, p=0.07) in the subgroup of women with three or more miscarriages. Investigation of 1691 G>A polymorphism showed preponderance of genotype GA (12.50% vs 6,51% , p=0.31) and allele A (6.25% vs 3.25% , p=0,31) in a group of women with miscarriages between 10 and 13 week of gestation in comparison to the control group. Investigation of 20210G>A polymorphism showed majority of genotype GA (8.70%) in women with miscarriages in the early as well as in the late period of the first trimester in comparison to the control group (1.18%, p=0.07). The frequency of occurrance of mutated allele A was 4.35% and 0.59% in the control group (1.18%, p=0.07). Conclusions: The analysis of obtained results suggests the possible influence of both considered polymorphisms 1691G>A of factor V gene and 20210G>A prothrombin gene on mechanism of recurrent miscarriages in the first trimester of pregnancy and participation of 1691G>A polymorphism in the etiology of recurrent miscarriages during the late period of the first trimester of pregnancy (10-13 week of gestation)

Genetic conditioned changes in activity of 5,10-methylenetetrahydrofolate reductase (MTHFR) and recurrent miscarriages

Summary 5, 10-methylenetetrahydrofolate reductase (MTHFR) is the key enzyme in folate, methionine and homocysteine metabolism. The disturbances in MTHFR activity could be the cause of increased serum level of homocysteine. Hyperhomocysteinemia is a risk factor of changes in coagulation cascade through direct cytotoxic influence on endothelium, atherogenesis, activation of coagulation factor V and VII, increased level of thrombin and platelets aggregation. Genetic disturbances in MTHFR enzyme activity in the presence of polymorphic variants of its gene are responsible for homocysteine augmentation and could be the reason of several gestational complications such as recurrent miscarriages

Znaczenie polimorfizmu CYP1A1 i regulacji jego transkrypcji w podatności na raka jajnika i endometrium

Abstract Human cytochrome P450 1A1 is one of the most important enzymes participating in human carcinogenesis because it metabolites several procarcinogens to active carcinogenic metabolites. Additionally, enzymes of CYP450 family play an important role in estrogenes catabolization (17-β-estradiol and estron) to intermediate products (2-, 4-hydroxyestradiol and 2-, 4-hydroxyestrone) including CYP1A1 that catalyses hydroxylation to 2-hydroxyestrogens in the endometrium. Derivates of these compounds (4-hydroxyestrogens) are carcinogenic and could induce DNA damage leading to tumour transformation. The presence of CYP1A1 enzyme in genital tract tissues could induce chemical carcinogenesis initiating cancer development. Recent studies also confirmed the role of CYP1A1 in the development of ovarian and endometrial cancer in humans. The presence of mutated CYP1A1 polymorphic variants influencing the CYP1A1 activity could be responsible for different interindividual susceptibility to genital cancers in women.Streszczenie Ludzki cytochrom CYP4501A1 jest jednym z najważniejszych enzymów uczestniczących w procesie kancerogenezy, ponieważ metabolizuje wiele pro-kancerogenów do ich aktywnych kancerogennych metabolitów. Dodatkowo, enzymy z rodziny CYP450 odgrywają ważną rolę w katabolizmie estrogenów (17-β-estradiol i estron) do produktów pośrednich (2-, 4-hydroksyestradiol oraz 2-, 4-hydroksyestron) uwzględniając CYP1A1, który katalizuje hydroksylację do 2-hydroksyestrogenu w endometrium. Pochodne tych związków (4-hydroksyestrogeny) są kancerogenne i mogą indukować uszkodzenia DNA prowadząc do transformacji nowotworowej komórki. Obecność enzymu CYP1A1 w tkankach narządów płciowych może indukować chemiczną kancerogenezę inicjując rozwój nowotworu. Najnowsze badania potwierdziły rolę CYP1A1 w rozwoju raka jajnika i endometrium. Obecność zmutowanych wariantów polimorficznych genu CYP1A1 wpływających na aktywność CYP1A1 może być związana ze zróżnicowaniem osobniczym w podatności na nowotwory narządów płciowych u kobiet

Znaczenie polimorfizmów rs1021737 i rs482843 gamma-liazy cystationinowej w etiologii stanu przedrzucawkowego w populacji kaukaskiej

Introduction: Recently, an increasing number of reports indicate the participation of genetic factors in the pathogenesis of preeclampsia (PE). The genes involved in the synthesis of nitric oxide that participates in the vasolidation, may play an important role in the development of this disorder. Hydrogen sulfide (H2S) which is produced by cystathionine gamma-lyase exhibits a similar effect to nitric oxide. It is suggested that certain polymorphisms of the CTH gene may participate in the development of chronic hypertension and preeclampsia. Aim of the study: To evaluate the frequency of genotypes and alleles of rs1021737 and rs482843 polymorphisms of CTH gene in women with preeclampsia from Wielkopolska region. Material and methods: The study group consisted of 60 patients with diagnosed preeclampsia, into the control group 120 healthy pregnant women were enrolled. The examined rs1021737 and rs482843 polymorphisms of CTH gene were determined using PCR-RFLP method. Results: Analysis of rs482843 polymorphism in the CTH gene showed a statistically significant difference in the prevalence of mutated GG genotype (pWstęp: W ostatnim czasie coraz więcej doniesień wskazuje na udział czynników genetycznych w etiopatogenezie stanu przedrzucawkowego (PE – preeclampsia). Istotną rolę mogą odgrywać geny uczestniczące w syntezie tlenku azotu, głównego wazodylatora naczyń. Podobne działanie wazodyletacyjne pełni także siarkowodór (H2S) syntetyzowany przy udziale gamma-liazy cystationinowej. Sugeruje się, że określone polimorfizmy genu CTH mogą mieć znaczenie w rozwoju nadciśnienia przewlekłego i stanu przedrzucawkowego. Cel pracy: Określenie częstości występowania genotypów i alleli polimorfizmów rs1021737 i rs482843 genu CTH w grupie kobiet ze stanem przedrzucawkowym z regionu Wielkopolski. Materiał i metody: Grupa badana obejmowała 60 pacjentek ze zdiagnozowanym stanem przedrzucawkowym, natomiast grupa kontrolna stanowiła 120 zdrowych kobiet ciężarnych. Analizowane polimorfizmy rs1021737 i rs482843 genu CTH oznaczono przy pomocy metody PCR-RFLP. Wyniki: Analiza polimorfizmu rs482843 genu CTH wykazała różnicę istotną statystycznie w częstości występowania genotypu zmutowanego GG (

Frequency of G2677T/A and C3435T polymorphisms of MDR1 gene in preeclamptic women

Objective: Preeclampsia (PE) belongs to main causes of mortality rates of mothers, fetuses and new born children. Polymorphism of MDR1 gene is connected with reduction of P-glycoprotein expression in placenta and increased fetal exposure to xenobiotics. The aim of the study was to determine the frequency of C3435T and G2677T/A polymorphisms of MDR1 gene in pregnant women with preeclampsia. Materials and methods: The study consisted of 180 Polish women including 60 women with PE and 120 healthy pregnant women. Determination of C3435T and G2677T/A polymorphisms of MDR1 gene was performed using PCR-RFLP method. Results: No significant association between genotypes of the examined polymorphisms and the clinical parameters of pregnant women with PE was observed. However the interesting tendency to higher prevalence of mutated 2677A allele of G2677T/A MDR1 polymorphism in PE group has been shown (2,50 vs. 0,83% in controls, OR=3,05, ns). Conclusions: The results of this study suggest no significant effect of examined C3435T and G2677T/A MDR1 polymorphisms in PE pathogenesis. However given the noteworthy results related to mutated 2677A allele of G2677T/A MDR1 polymorphism in preeclamptic women further studies seem to be needed. Nevertheless, the frequency of investigated polymorphisms was consistent with the distribution in other Caucasian populations

Polimorfizm genu kodującego osteoprotegerynę a występowanie osteoporozy u kobiet po menopauzie

Summary Introduction: Osteoprotegerin (OPG) plays a crucial role in the control of bone resorption through competitive inhibition of receptor activator of nuclear factor κB ligand (RANKL). This process leads to inhibition of osteoclasts differentiation and activity. The aim of the following study was to evaluate the distribution of genotypes of -163A>G and 1181G>C polymorphisms in OPG gene, and analyze their relationship with bone mineral density (BMD) and other parameters of bone turnover in population of Polish postmenopausal women. Material and method: The study included 310 postmenopausal Caucasian women (54,48}8,53 years); 139 women with osteoporosis, 107 with osteopenia and 64 healthy women. Genetic analysis was performed by PCR/RFLP reaction. BMD value was measured by dual energy X-ray absorptiometry (DXA). Results: For -163A>G polymorphism the higher frequency of heterozygotes AG (26.6 vs. 18.7%, ns) and slight overrepresentation of mutated G allel (14,1 vs. 10,9%, ns) in the osteoporosis group was observed. The frequency of recessive CC homozygotes and C alleles of 1181G>C polymorphism did not differ among the investigated groups. The distribution of particular haplotypes of -163A>G and 1181G>C polymorphisms in all subgroups was similar. Correlation between values of investigated parameters of bone turnover and frequency of genotypes of investigated polymorphisms has not been observed. Conclusions: The overrepresentation of heterozygous AG genotype and mutated G allele of -163A>G polymorphism of OPG gene in the group of women with osteoporosis might suggest the significance of this variant in the development of osteoporosis. A more extensive analysis of genetic variants of RANKL/RANK/OPG signal pathways, joint with an investigation of modulated influence of estrogens, TNF-α or several interleukin influencing the development of osteoporosis is necessary.Streszczenie Wstęp: Białko osteoprotegeryna (OPG) pełni kluczowa rolę w kontroli resorpcji kości blokując kompetencyjnie ligan jądrowego czynnika aktywującego receptor κB (RANKL). Proces ten prowadzi do hamowania różnicowania i aktywności osteoklastów. Celem badania była ocena rozkładu genotypów polimorfizmów -163A>G oraz 1181G>C genu OPG, a także analiza związku genotypów z gęstością mineralną kości (BMD) oraz innych parametrów obrotu kostnego w populacji kobiet polskich po menopauzie. Materiał i metoda: Badaniem objęto 310 niespokrewnionych kobiet rasy kaukaskiej (54,48+/-8,53 lata) po menopauzie (139 kobiet z osteoporoza, 107 kobiet z osteopenią, 64 zdrowych kobiet). Analizę genetyczna przeprowadzono przy pomocy reakcji PCR/RFLP. Pomiar BMD wykonano przy zastosowaniu metody podwójnej absorpcjometrii rentgenowskiej (DXA). Wyniki: W przypadku polimorfizmu -163A>G zauważono częstsze występowanie heterozygot AG (26,6 vs 18,7%, ns) oraz nieznacznie wyższą częstość występowania zmutowanego allela G (14,1 vs 10,9%, ns) w grupie kobiet z osteoporoza. Częstość występowania homozygot recesywnych CC oraz alleli C polimorfizmu 1181G>C nie wykazywała różnic pomiędzy badanymi podgrupami. Rozkład występowania poszczególnych haplotypów polimorfizmów -163A>G oraz 1181G>C we wszystkich podgrupach był podobny. Nie zaobserwowano korelacji pomiędzy wartościami badanych parametrów obrotu kostnego a częstością pojawiania się poszczególnych genotypów badanych polimorfizmów w analizowanych podgrupach. Wnioski: Częstsze występowanie heterozygot AG oraz zmutowanego allela G polimorfizmu -163A>G genu OPG w grupie kobiet z osteoporoza może sugerować znaczenie tego wariantu w rozwoju osteoporozy. Wskazana jest szersza analiza wariantów genetycznych szlaku sygnałowego RANKL/RANK/OPG łącznie z badaniem modulacyjnego wpływu estrogenów, TNF-α oraz niektórych interleukin na rozwój osteoporozy