Investigation of the nutritional requirements of Australian snapper Pagrus auratus (Bloch & Schneider, 1801): effects of digestible energy content on utilization of digestible protein

This study used a curvilinear model to investigate the effects of different digestible energy (DE) levels on the digestible protein (DP) requirements of juvenile snapper Pagrus auratus. For each DE level (15, 18 or 21 MJ kg(-1)), DP content was increased from about 210-560 g kg(-1) in seven evenly spaced increments by formulating a summit diet (highest DP content) and a diluent diet (lowest DP content) at the desired DE level and combining the summit and diluent diets in various ratios to achieve the desired DP content. This ensured the DE level remained relatively stable. Each of the 21 dietary treatments was fed to three replicate groups of snapper twice daily to apparent satiation for 57 days. At the completion of the trial, fish were weighed and killed for chemical analysis. Results indicated that the rapid growth of snapper weighing 30-90 g was highly dependent on the ratio of DP to DE and that optimum protein deposition did not occur until snapper were offered feeds with at least 350 g DPkg(-1), irrespective of DE level. According to the fitted models, diets formulated for snapper reared at temperatures from 20-25 degrees C should contain approximately 23 g DP MJ DE-1 to promote optimal weight gain and protein deposition. Based on the feeding regime used in this study, this could be achieved with practical diets containing a DP:DE ratio of 460:20, 420:18 or 350:15