Comprehensive autoantibody profiling in systemic autoimmunity by a highly-sensitive multiplex protein array

Comprehensive autoantibody evaluation is essential for the management of autoimmune disorders. However, conventional methods suffer from poor sensitivity, low throughput, or limited availability. Here, using a proteome-wide human cDNA library, we developed a novel multiplex protein assay (autoantibody array assay; A-Cube) covering 65 antigens of 43 autoantibodies that are associated with systemic sclerosis (SSc) and polymyositis/dermatomyositis (PM/DM). The performance of A-Cube was validated against immunoprecipitation and established enzyme-linked immunosorbent assay. Further, through an evaluation of serum samples from 357 SSc and 172 PM/DM patients, A-Cube meticulously illustrated a diverse autoantibody landscape in these diseases. The wide coverage and high sensitivity of A-Cube also allowed the overlap and correlation analysis between multiple autoantibodies. Lastly, reviewing the cases with distinct autoantibody profiles by A-Cube underscored the importance of thorough autoantibody detection. Together, these data highlighted the utility of A-Cube as well as the clinical relevance of autoantibody profiles in SSc and PM/DM

Early improvement of nailfold videocapillaroscopy abnormalities in dermatomyositis patients with anti‐NXP‐2 antibody

Abstract This study aimed to evaluate long‐term changes in nailfold videocapillaroscopy (NVC) findings in dermatomyositis patients with antinuclear matrix protein 2 (NXP‐2) antibody (Ab). All four patients with anti‐NXP‐2 Ab presented irregularly enlarged and reduced capillaries and hemorrhages at the initial assessment. After disease stabilization, irregularly enlarged capillaries and hemorrhages disappeared within the mean observation period of 6 months. These early improvements were not observed in patients with anti‐TIF1 Ab. The results of this study show that long‐term changes in NVC findings should be assessed using myositis‐specific Ab information

IOD and Observed Air-sea Interaction in the Eastern Indian Ocean

http://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr11-01/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr14-01/

Seasonal and Interannual Variation in the Cross-equatorial Meridional Currents Observed in the Eastern Indian Ocean

http://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr00-k07_leg1/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr01-k05_leg1/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr02-k04_leg1/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr03-k03_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr04-03_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr05-03_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr06-05_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr07-07_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/kaiyo/ky09-01_leg2/

東部赤道インド洋で観測された南北流とその季節変動

http://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr00-k07_leg1/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr01-k05_leg1/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr02-k04_leg1/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr03-k03_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr04-03_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr05-03_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr06-05_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr07-07_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/kaiyo/ky09-01_leg2/

ADCPの長期観測から明らかになった東部赤道インド洋の南北流とその季節・経年変動

http://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr00-k07_leg1/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr01-k05_leg1/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr02-k04_leg1/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr03-k03_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr04-03_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr05-03_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr06-05_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr07-07_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/kaiyo/ky09-01_leg2/