3,995 research outputs found
Casimir Effect in the Rainbow Einstein's Universe
In the present paper we investigate the effects caused by the modification of
the dispersion relation obtained by solving the Klein-Gordon equation in the
closed Einstein's universe in the context of rainbow's gravity models. Thus, we
analyse how the quantum vacuum fluctuations of the scalar field are modified
when compared with the results obtained in the usual General Relativity
scenario. The regularization, and consequently the renormalization, of the
vacuum energy is performed adopting the Epstein-Hurwitz and Riemann's zeta
functions.Comment: 15 pages, 1 figure. To appear in Europhysics Letter
Fluorescent Plasmodium berghei sporozoites and pre-erythrocytic stages: a new tool to study mosquito and mammalian host interactions with malaria parasites.
To track malaria parasites for biological studies within the mosquito and mammalian hosts, we constructed a stably transformed clonal line of Plasmodium berghei, PbFluspo, in which sporogonic and pre-erythrocytic liver-stage parasites are autonomously fluorescent. A cassette containing the structural gene for the FACS-adapted green fluorescent protein mutant 2 (GFPmut2), expressed from the 5' and 3' flanking sequences of the circumsporozoite (CS) protein gene, was integrated and expressed at the endogenous CS locus. Recombinant parasites, which bear a wild-type copy of CS, generated highly fluorescent oocysts and sporozoites that invaded mosquito salivary glands and were transmitted normally to rodent hosts. The parasites infected cultured hepatocytes in vitro, where they developed into fluorescent pre-erythrocytic forms. Mammalian cells infected by these parasites can be separated from non-infected cells by fluorescence activated cell sorter (FACS) analysis. These fluorescent insect and mammalian stages of P. berghei should be useful for phenotypic studies in their respective hosts, as well as for identification of new genes expressed in these parasite stages
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