USCID fourth international conference

Presented at the Role of irrigation and drainage in a sustainable future: USCID fourth international conference on irrigation and drainage on October 3-6, 2007 in Sacramento, California.Includes bibliographical references.Application of different irrigation management practices plays a considerable role in water saving to achieve potential yields. On the other hand, network water distribution schedule is a governing factor in this regard. In current study conducted in Mahabad plain in North West of Iran, four different irrigation managements on sugarbeet cultivation including traditional farmer's management, Furrow Deepening, Reduced Discharge per Deepened Furrow, and Alternate Furrow Irrigation have been studied in real farmers' fields measuring 10.2 hectares. Participatory management approach has been used while working in farmers' fields. Soils textures are silty clayey. Results of studies indicate that water used has been reduced considerably while higher root and sugar yields are obtained due to better on-farm water management practices. Water Use Efficiency, in kg of yield per m3 of water used, increased considerably under alternate furrow irrigation management in comparison to what obtained under traditional management. Results show application of alternate furrow irrigation in sugarbeet cultivation not only resulted in lesser water use per hectare, but also it increased both root and sugar yields and, consequently, higher water use efficiency was obtained. Assessments have been made on irrigation schedule imposed by the irrigation network and its effects on actual water requirements. Results show that the delivery schedule practiced in the network in incapable of delivering the actual amount of water requirement for the dominant crop of the scheme. Suggestions are made to the network operator to improve overall network efficiency including revisions on water resources planning and allocation and/or improve network operation system

Phenotypic modulation of auto-reactive cells by insertion of tolerogenic molecules via MSC-derived exosomes

Auto-reactive cells-mediated immune responses are responsible for the current tissue damages during autoimmunity. Accordingly, functional modulation of auto-reactive cells has been a pivotal aim in many of recent studies. In the current study, we investigated the possibility for insertion of regulatory molecules onto auto-reactive cells through exosomal nano-shuttles as a novel approach for phenotype modification of auto-reactive cells. The exosomes were isolated from supernatant of mesenchymal stem cells culture. Resultant exosomes co-cultured with lymphocytes were harvested from established EAE mice in the presence of antigenic MOG35-55 peptide. After 24 hr, insertion of exosomal tolerogenic molecules (PD-L1, TGF-β, galectin-1) onto auto-reactive cells were explored through flow cytometry. The potency of exosomal inserted membrane molecules to modulate phenotype of auto-reactive lymphocytes was assessed upon ELISA test for their-derived cytokines IFN-γ and IL-17. Incorporation of exosomal molecules into lymohocytes’ membrane was confirmed by flow cytometric analyses for surface levels of mentioned molecules. Additionally, the decreased secretion of IFN-γ and IL-17 were detected in exosome pre-treated lymphocytes upon stimulation with MOG peptide. Mesenchymal stem cells -derived exosomes showed to be efficient organelles for insertion of bioactive tolerogenic molecules onto auto-reactive cells and modulation of their phenotypes