Implantation Serine Proteinase 1 Exhibits Mixed Substrate Specificity that Silences Signaling via Proteinase-Activated Receptors

Implantation S1 family serine proteinases (ISPs) are tryptases involved in embryo hatching and uterine implantation in the mouse. The two different ISP proteins (ISP1 and ISP2) have been detected in both pre- and post-implantation embryo tissue. To date, native ISP obtained from uterus and blastocyst tissues has been isolated only as an active hetero-dimer that exhibits trypsin-like substrate specificity. We hypothesised that in isolation, ISP1 might have a unique substrate specificity that could relate to its role when expressed alone in individual tissues. Thus, we isolated recombinant ISP1 expressed in Pichia pastoris and evaluated its substrate specificity. Using several chromogenic substrates and serine proteinase inhibitors, we demonstrate that ISP1 exhibits trypsin-like substrate specificity, having a preference for lysine over arginine at the P1 position. Phage display peptide mimetics revealed an expanded but mixed substrate specificity of ISP1, including chymotryptic and elastase activity. Based upon targets observed using phage display, we hypothesised that ISP1 might signal to cells by cleaving and activating proteinase-activated receptors (PARs) and therefore assessed PARs 1, 2 and 4 as potential ISP1 targets. We observed that ISP1 silenced enzyme-triggered PAR signaling by receptor-disarming. This PAR-disarming action of ISP1 may be important for embryo development and implantation

Reviews : Los seys libros del Delphín de música de cifra para tañer la vihuela __ de Luys Narváez

Es separata de: "Hispanic Review, v. XVI, 1948"É separata de: Hispanic Review, v. XVI, 1948Aut. tomado de fin de text

Criterio objetivo para determinar a autoria e a cronologia na dramatica espanhola

Es separata de: Revista do Arquivo, n. LVIIÉ separata de: Revista do Arquivo, n. LVI

The curious phenomenon of Spanish verse drama

Griswold Morley S. The curious phenomenon of Spanish verse drama. In: Bulletin Hispanique, tome 50, n°3-4, 1948. pp. 445-462

James Fitzmaurice-Kelly, A New Hislory of Spanish Literature.

Griswold Morley S. James Fitzmaurice-Kelly, A New Hislory of Spanish Literature.. In: Bulletin Hispanique, tome 30, n°3, 1928. pp. 269-276

The use of the verse-forms (strophes) by Tirso de Molina

Griswold Morley S. The use of the verse-forms (strophes) by Tirso de Molina. In: Bulletin Hispanique, tome 7, n°4, 1905. pp. 387-408

El uso de las combinaciones métricas en las comedias de Tirso de Molina

Griswold Morley S. El uso de las combinaciones métricas en las comedias de Tirso de Molina. In: Bulletin Hispanique, tome 16, n°2, 1914. pp. 177-208

The accentuation of the past participles in -uido

Es separata de: "Hispania, v.4, october, 1921"É separata de: Hispania, v.4, october, 192

A note on arabic poetry and european poetry

Es separata de: "Hispanic Review, v. VII, 1939"É separata de: Hispanic Review, v. VII, 1939Aut. tomado de fin de text