Establishment of an antibody specific for cancer-associated haptoglobin: a possible implication of clinical investigation

We previously found that the serum level of fucosylated haptoglobin (Fuc-Hpt) was significantly increased in pancreatic cancer patients. To delineate the mechanism underlying this increase and develop a simple detection method, we set out to generate a monoclonal antibody (mAb) specific for Fuc-Hpt. After multiple screenings by enzyme-linked immunosorbent assay (ELISA), a 10-7G mAb was identified as being highly specific for Fuc-Hpt generated in a cell line as well as for Hpt derived from a pancreatic cancer patient. As a result from affinity chromatography with 10-7G mAb, followed by lectin blot and mass spectrometry analyses, it was found that 10-7G mAb predominantly recognized both Fuc-Hpt and prohaptoglobin (proHpt), which was also fucosylated. In immunohistochemical analyses, hepatocytes surrounding metastasized cancer cells were stained by the 10-7G mAb, but neither the original cancer cells themselves nor normal hepatocytes exhibited positive staining, suggesting that metastasized cancer cells promote Fuc-Hpt production in adjacent hepatocytes. Serum level of Fuc-Hpt determined with newly developed ELISA system using the 10-7G mAb, was increased in patients of pancreatic and colorectal cancer. Interestingly, dramatic increases in Fuc-Hpt levels were observed at the stage IV of colorectal cancer. These results indicate that the 10-7G mAb developed is a promising antibody which recognizes Fuc-Hpt and could be a useful diagnostic tool for detecting liver metastasis of cancer.This study was performed as a research program of the Project for Development of Innovative Research on Cancer Therapeutics (P-Direct), Ministry of Education, Culture, Sports, Science and Technology of Japan and was supported by JSPS KAKENHI Grant Number JP16H05226

Global Consensus Statement; How Can Judo Contribute to Reducing the Problem of Injurious Falls in Older Adults?

The Global Consensus Statement on judo-based interventions for older adults addresses the potential of judo techniques, particularly ukemi (safe falling techniques), in minimising the risk and impact of falls among the elderly. Falls represent a significant health challenge for the ageing population, contributing to injuries and high healthcare costs. The statement outlines best practice and recommendations for implementing judo-based fall prevention programmes globally. These programmes leverage judo's multi-faceted approach, incorporating strength, balance, co-ordination, and safe landing skills to reduce the severity of falls and minimise the fear of falling. Research indicates that older adults can learn and retain these motor skills, enhancing their ability to fall safely and reduce injury risks. The consensus also highlights the importance of collaboration between judo federations, healthcare providers and community organisations to scale these interventions effectively. By promoting judo-based exercise programmes, the consensus aims to improve physical, psychological and social wellbeing among older adults, ultimately enhancing their independence and quality of life.Peer reviewe

“Judo Health Exercise” development and planning to spread throughout Japan

J-GLOBAL ID : 200901043284363204J-GLOBAL ID : 200901004099122068J-GLOBAL ID : 202201010240591568J-GLOBAL ID : 201001039879999076J-GLOBAL ID : 200901050705583957We have developed a physical exercise named “Judo Health Exercise” base on “Judo” throwing techniques such as “Seoi-nage”, “O-goshi”, “O-soto-gari”, “Uchimata”, and “Ashi-harai”. This exercise aims at prevention of dementia and that of fall down. These throwing techniques mention above, include the motion of the body rotation and the swing leg motion. The motion of the body rotation is visually clear, but to make it certain, we have examined upper body rotation by using an accelerometer and a gyroscope. The gyroscope showed the clear body rotation. We have compared this “Judo Health Exercise” to the traditional and widely spread “Radio Exercise No.1”. By this examination. we are able to conclude “Judo Health Exercise” is developed mainly for moving the lower part of the human body. On the other hand, “Radio Exevcise No.1” was developed mainly for moving the upper part of the human body. We have also examined the emotional difference between before practicing “Judo Health Exercise” after practicing it. For this examination, We have used a questionnaire. We have depicted the motion of “Judo Health Exercise”. In the future, we are planning “Judo Health Exercise” to spread throughout Japan.1520297128944766464application/pdfdepartmental bulletin pape

High-Dose Irradiation Inhibits Motility and Induces Autophagy in Caenorhabditis elegans

Radiation damages many cellular components and disrupts cellular functions, and was previously reported to impair locomotion in the model organism Caenorhabditis elegans. However, the response to even higher doses is not clear. First, to investigate the effects of high-dose radiation on the locomotion of C. elegans, we investigated the dose range that reduces whole-body locomotion or leads to death. Irradiation was performed in the range of 0–6 kGy. In the crawling analysis, motility decreased after irradiation in a dose-dependent manner. Exposure to 6 kGy of radiation affected crawling on agar immediately and caused the complete loss of motility. Both γ-rays and carbon-ion beams significantly reduced crawling motility at 3 kGy. Next, swimming in buffer was measured as a motility index to assess the response over time after irradiation and motility similarly decreased. However, swimming partially recovered 6 h after irradiation with 3 kGy of γ-rays. To examine the possibility of a recovery mechanism, in situ GFP reporter assay of the autophagy-related gene lgg-1 was performed. The fluorescence intensity was stronger in the anterior half of the body 7 h after irradiation with 3 kGy of γ-rays. GFP::LGG-1 induction was observed in the pharynx, neurons along the body, and the intestine. Furthermore, worms were exposed to region-specific radiation with carbon-ion microbeams and the trajectory of crawling was measured by image processing. Motility was lower after anterior-half body irradiation than after posterior-half body irradiation. This further supported that the anterior half of the body is important in the locomotory response to radiation