Production of antisera against sterigmatocystin and its use in an enzyme-linked immunosorbent assay for sterigmatocystin in barley

An enzyme-linked immunosorbent assay for the detection of 3-acetyldeoxynivalenol has been set up with an antiserum preparation raised against a bovine serum albumin - 3-acetyldeoxynivalenol hemiglutarate conjugate. The assay is an indirect, double antibody immunoassay performed on microtitration plates, and is sensitive (with a limit of detection of 1 pg per well) and specific. The assay has been validated for application to rice with a sensitivity of 1 ng g-1. Sample extraction is a simple solubilisation procedure. The immunoassay described has a number of advantages over conventional methods of analysis

Aflatoxin determination in peanut butter by enzyme-linked immunosorbent assay

High specificity, high titre antisera to aflatoxins B1 and G1 have been produced by immunisation of rabbits with a bovine serum albumin-aflatoxin B1 conjugate. The antiserum has been used to set up an indirect, double antibody microtitration plate enzyme-linked immunosorbent assay with a limit of detection of 0.1 pg toxin per well. The assay has been validated for application to peanut butter, and demonstrates many advantages over conventional methodology, including the requirement for minimal sample preparation before assay, its technical simplicity, and its potential high sample through-put

Investigation of the relationship between in vitro ELISA measures of immunoreactive soy globulins and in vivo effects of soy products

International audienc

Duodenal passage of immunoreactive glycinin and beta-conglycinin from soya bean in preruminant calves

International audienc