Human derived feeder fibroblasts for the culture of epithelial cells for clinical use

Aim: To investigate human oral mucosal fibroblasts (HOMF) and human limbal fibroblasts (HLF) as alternatives to murine 3T3 feeder fibroblasts currently used to support epithelial cell expansion for the treatment of limbal epithelial stem cell deficiency. Methods: HLF and HOMF were compared to 3T3 for their ability to support the culture of human limbal epithelial cells (HLE) and human oral mucosal epithelial cells. Results: HOMF, but not HLF, were equivalent to 3T3 in terms of the number of epithelial population doublings achieved. HLE co-cultured with HOMF or 3T3 had similar expression of corneal and putative stem cell markers. Conclusion: HOMF are a suitable and safer feeder fibroblast alternative to 3T3 for the production of epithelial cells for clinical use

Model for nucleation in GaAs homoepitaxy derived from first principles

The initial steps of MBE growth of GaAs on beta 2-reconstructed GaAs(001) are investigated by performing total energy and electronic structure calculations using density functional theory and a repeated slab model of the surface. We study the interaction and clustering of adsorbed Ga atoms and the adsorption of As_2 molecules onto Ga atom clusters adsorbed on the surface. The stable nuclei consist of bound pairs of Ga adatoms, which originate either from dimerization or from an indirect interaction mediated through the substrate reconstruction. As_2 adsorption is found to be strongly exothermic on sites with a square array of four Ga dangling bonds. Comparing two scenarios where the first As_2 gets incorporated in the incomplete surface layer, or alternatively in a new added layer, we find the first scenario to be preferable. In summary, the calculations suggest that nucleation of a new atomic layer is most likely on top of those surface regions where a partial filling of trenches in the surface has occurred before.Comment: 8 pages, 14 figures, Submitted to Phys. Rev. B (December 15, 1998). Other related publications can be found at http://www.fhi-berlin.mpg.de/th/paper.htm

Fast Transition between High-soft and Low-soft States in GRS 1915+105: Evidence for a Critically Viscous Accretion Flow

We present the results of a detailed analysis of RXTE observations of class $\omega$ which show an unusual state transition between high-soft and low-soft states in the microquasar GRS 1915+105. Out of about 600 pointed RXTE observations, the source was found to exhibit such state transition only on 16 occasions. An examination of the RXTE/ASM data in conjunction with the pointed observations reveals that these events appeared as a series of quasi-regular dips in two stretches of long duration (about 20 days during each occasions) when hard X-ray and radio flux were very low. The X-ray light curve and color-color diagram of the source during these observations are found to be different from any reported so far. The duration of these dips is found to be of the order of a few tens of seconds with a repetition time of a few hundred seconds. The transition between these dips and non-dips which differ in intensity by a factor of ~ 3.5, is observed to be very fast (~ a few seconds). It is observed that the low-frequency narrow QPOs are absent in the power density spectrum (PDS) of the dip and non-dip regions of class $\omega$ and the PDS is a power law in 0.1 - 10 Hz frequency range. There is a remarkable similarity in the spectral and timing properties of the source during the dip and non-dip regions in these set of observations. These properties of the source are distinctly different from those seen in the observations of other classes. This indicates that the basic accretion disk structure during both dip and non-dip regions of class $\omega$ is similar, but differ only in intensity. To explain these observations, we invoke a model in which the viscosity is very close to critical viscosity and the shock wave is weak or absent.Comment: Replaced with correct figures, Jour. of Astrophysics and Astronomy (accepted

The viscous disk of GRS 1915+105

GRS 1915+105, one of the two known galactic microquasars, shows an extremely complex variability in the X-ray band, comparable to no other X-ray source in the sky. Making use of RXTE/PCA data, we have analyzed the X-ray spectral distribution throughout the variability. We find that all variations can be attributed to the rapid appearance and disappearance of the inner region of an optically-thick accretion disk. Since the time scale for each event is related to the maximum radius of the disappearing region, the difference in time structure is due to the time distribution of such radii. The observed relation between the extent of the missing inner region of the disk and the duration of an event is in remarkable agreement with the expected radius dependence of the viscous time scale of the radiation-dominated region of an accretion disk.Comment: 6 pages, 3 figures. To appear in The Active X-ray Sky: Results from BeppoSAX and Rossi-XTE, Nuclear Physics B Proceedings Supplements, L. Scarsi, H. Bradt, P. Giommi and F. Fiore (eds.), Elsevier Science B.

Inferring Loop Invariants using Postconditions

One of the obstacles in automatic program proving is to obtain suitable loop invariants. The invariant of a loop is a weakened form of its postcondition (the loop's goal, also known as its contract); the present work takes advantage of this observation by using the postcondition as the basis for invariant inference, using various heuristics such as "uncoupling" which prove useful in many important algorithms. Thanks to these heuristics, the technique is able to infer invariants for a large variety of loop examples. We present the theory behind the technique, its implementation (freely available for download and currently relying on Microsoft Research's Boogie tool), and the results obtained.Comment: Slightly revised versio

A study on the sensitivities of simulated aerosol optical properties to composition and size distribution using airborne measurements

We present a flexible framework to calculate the optical properties of atmospheric aerosols at a given relative humidity based on their composition and size distribution. The similarity of this framework to climate model parameterisations allows rapid and extensive sensitivity tests of the impact of uncertainties in data or of new measurements on climate relevant aerosol properties. The data collected by the FAAM BAe-146 aircraft during the EUCAARI-LONGREX and VOCALS-REx campaigns have been used in a closure study to analyse the agreement between calculated and measured aerosol optical properties for two very different aerosol types. The agreement achieved for the EUCAARI-LONGREX flights is within the measurement uncertainties for both scattering and absorption. However, there is poor agreement between the calculated and the measured scattering for the VOCALS-REx flights. The high concentration of sulphate, which is a scattering aerosol with no absorption in the visible spectrum, made the absorption measurements during VOCALS-REx unreliable, and thus no closure study was possible for the absorption. The calculated hygroscopic scattering growth factor overestimates the measured values during EUCAARI-LONGREX and VOCALS-REx by ∼30% and ∼20%, respectively. We have also tested the sensitivity of the calculated aerosol optical properties to the uncertainties in the refractive indices, the hygroscopic growth factors and the aerosol size distribution. The largest source of uncertainty in the calculated scattering is the aerosol size distribution (∼35%), followed by the assumed hygroscopic growth factor for organic aerosol (∼15%), while the predominant source of uncertainty in the calculated absorption is the refractive index of organic aerosol (28–60%), although we would expect the refractive index of black carbon to be important for aerosol with a higher black carbon fraction

Paradoxical family practices: LGBTQ+ young people, mental health and wellbeing

This article will explore how LGBTQ+ young people sustain, and in some cases survive, family relationships. We develop the concept of ‘paradoxical family practices’ and use this to demonstrate the ways in which LGBTQ+ young people manage family life through everyday emotion work. This highlights: (1) how families ordinarily navigate heteronormativity and ‘issues’ of gender/sexuality; (2) the efficacy of ‘paradoxical family practices’ as a conceptual tool; (3) the value of emotion-centred multiple qualitative methods to explore the lives of LGBTQ+ young people and mental health. Findings derive from a small-scale UK study funded by the Wellcome Trust (UNS39780) and were generated through a two-stage methodology comprising digital/paper emotion maps and qualitative interviews with LGBTQ+ young people aged 16–25 (n = 12) followed by diary methods and follow-up interviews (n = 9). Interviews were also completed with ‘family members’ (n = 7)

Origins of the Ambient Solar Wind: Implications for Space Weather

The Sun's outer atmosphere is heated to temperatures of millions of degrees, and solar plasma flows out into interplanetary space at supersonic speeds. This paper reviews our current understanding of these interrelated problems: coronal heating and the acceleration of the ambient solar wind. We also discuss where the community stands in its ability to forecast how variations in the solar wind (i.e., fast and slow wind streams) impact the Earth. Although the last few decades have seen significant progress in observations and modeling, we still do not have a complete understanding of the relevant physical processes, nor do we have a quantitatively precise census of which coronal structures contribute to specific types of solar wind. Fast streams are known to be connected to the central regions of large coronal holes. Slow streams, however, appear to come from a wide range of sources, including streamers, pseudostreamers, coronal loops, active regions, and coronal hole boundaries. Complicating our understanding even more is the fact that processes such as turbulence, stream-stream interactions, and Coulomb collisions can make it difficult to unambiguously map a parcel measured at 1 AU back down to its coronal source. We also review recent progress -- in theoretical modeling, observational data analysis, and forecasting techniques that sit at the interface between data and theory -- that gives us hope that the above problems are indeed solvable.Comment: Accepted for publication in Space Science Reviews. Special issue connected with a 2016 ISSI workshop on "The Scientific Foundations of Space Weather." 44 pages, 9 figure