Étude des tracas quotidiens des étudiants : liens avec la santé perçue

National audienceThis study shows, among Science, Literature and Human Science students (second year, N: 254, mean age 20,7 ± 2,3) that there is a link between daily hassles and state of health perceived. This link to the detriment of their subjective quality of life. A self administration questionnaire was elaborated (test-group N: 60) and then validated. The sensitivity (gaussian distribution), the concurrent validity (correlation (r) on global score: General Health Questionnaire–28, 0,35; State-Trait Anxiety Inventory-Y, dimension: 0,45, state: 0,42 ; World Health Organisation Quality Of Life –26, -0,43), the construct validity (r: 0.82 between impediment and frequency scores), the internal consistency (Cronbach’alpha: 0.70) are correct. The main hassles are fear of missing studies (because of a lack of capacity, of time, of money…), fear of unemployment, sensitivity about own security (robbery, aggression…) and other’s pain. On the other hand, affective life is less often given as a difficulty.Ce travail met en évidence, chez les étudiants en sciences, lettres et sciences humaines (2e année, N : 254, âge moyen : 20,7 ± 2,3), l’existence d’un lien entre les tracas quotidiens et l’état de santé perçue, cela au détriment de leur qualité de vie subjective. Un autoquestionnaire a été élaboré (groupe « test », N : 60) et ensuite validé. La sensibilité (distribution gaussienne), la validité concourante (Corrélation (r) sur le score total : Général Health Questionnaire –28, 0,35 ; State-Trait Anxiety Inventory-Y, trait : 0,45, état : 0,42 ; World Health Organisation Quality Of Life -26, -0,43), la validité de construction (r : 0,82 entre les scores de gêne et de fréquence) et la consistance interne (alpha de Cronbach : 0,70) sont satisfaisantes. Les principales préoccupations sont : la peur d’échouer dans les études (par manque de compétence, de temps, d’argent…), la peur du chômage, la sensibilité à la souffrance des autres et la sécurité personnelle (vol, agression…). En revanche, la vie affective est rarement citée comme étant une source de difficultés

IL-8 secretion in primary cultures of prostate cells is associated with prostate cancer aggressiveness

Bertrand Neveu*, Xavier Moreel*, Marie-Pier Deschênes-Rompré, Alain Bergeron, Hélène LaRue, Cherifa Ayari, Yves Fradet, Vincent FradetDepartment of Surgery, Laval University Cancer Research Centre, CHU de Quebec Research Centre, Quebec, QC, Canada *These authors contributed equally to this workBackground: Chronic inflammation is believed to be a major factor in prostate cancer initiation and promotion and has been studied using prostate cancer cells and immortalized cell lines. However, little is known about the contribution of normal cells to the prostatic microenvironment and inflammation. We aim to study the contribution of normal prostate epithelial cells to prostate inflammation and to link the inflammatory status of normal cells to prostate cancer aggressiveness.Materials and methods: Short-term primary cell cultures of normal epithelial prostate cells were derived from prostate biopsies from 25 men undergoing radical prostatectomy, cystoprostatectomy, or organ donation. Cells were treated with polyinosinic:polycytidylic acid, a mimic of double-stranded viral RNA and a potent inducer of the inflammatory response. Secretion of interleukin (IL)-8 in the cell culture medium by untreated and treated cells was measured and we determined the association between IL-8 levels in these primary cell cultures and prostate cancer characteristics. The Fligner–Policello test was used to compare the groups.Results: Baseline and induced IL-8 secretion were highly variable between cultured cells from different patients. This variation was not related to drug use, past medical history, age, or preoperative prostate-specific antigen value. Nonetheless, an elevated secretion of IL-8 from normal cultured epithelial cells was associated with prostate cancer aggressiveness (P=0.0005).Conclusion: The baseline secretion of IL-8 from normal prostate epithelial cells in culture is strongly correlated with cancer aggressiveness and may drive prostate cancer carcinogenesis. A better characterization of individual prostate microenvironment may provide a basis for personalized treatment and for monitoring the effects of strategies aimed at preventing aggressive prostate cancer.Keywords: cancer aggressiveness, inflammation, interleukin 8, primary cell cultur