The RNA polymerase II subunit Rpb4p mediates decay of a specific class of mRNAs

It is commonly appreciated that the mRNA level is determined by the balance between its synthetic and decay kinetics. Yet, little is known about coordination between these distinct processes. A major pathway of the eukaryotic mRNA decay initiates with shortening of the mRNA poly(A) tail (deadenylation), followed by removal of the mRNA 5′ cap structure and its subsequent exonucleolytic degradation. Here we report that a subunit of RNA polymerase II, Rpb4p, is required for the decay of a class of mRNAs whose products are involved in protein synthesis. Cells lacking RPB4 are defective in the deadenylation and post-deadenylation steps of representatives of this class of mRNAs. Moreover, Rpb4p interacts with both the mRNP and with subunits of the mRNA decay complex Pat1/Lsm1–7 that enhances decapping. Consistently, a portion of Rpb4p is localized in P bodies, where mRNA decapping and degradation is executed, and mutations in RPB4 increase the number of P bodies per cell. We propose that Rpb4p has a dual function in mRNA decay. It promotes or enhances the deadenylation process of specific mRNAs and recruits Pat1/Lsm1–7 to these mRNAs, thus stimulating their decapping and further decay. In this way, Rpb4p might link the activity of the basal transcription apparatus with that of the mRNA decay machinery

Creative Responses to Separation: Israeli and Palestinian Joint Activism in Bil\u27in

This article examines creative ways in which Israeli and Palestinian activists engage with each other and the powers seeking to separate them in their nonviolent struggles for a just and lasting peace. Using the geopolitical theory of territoriality, the article briefly examines a number of administrative, physical, and psychological barriers facing joint activism and the strategies activists use to counteract them. Drawing on nonviolent theory and practice, the article analyzes how activists exert power through the creative use of symbols and practices that undermine the legitimacy of occupation policies. Based on fieldwork conducted in 2004-05 and July 2006, the article explores the implications of this activism on conceptions of identity, and strategies for restarting a moribund peace process. The relative \u27success\u27 of sustained joint action in Bil\u27in can provide scholars and policymakers with innovative approaches for addressing some of the outstanding issues needing to be addressed by official negotiators. Although government bodies are more constrained than activists, the imaginative means of engaging with the system- and the reframing of issues through the redeployment of \u27commonplaces\u27-can perhaps provide inspiration, if not leverage, for thinking outside of the box