63,744 research outputs found

    Large space antenna technology applied to radar-imaging, rain-rate measurements, and ocean wind sensing

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    During the last decade, the utility of spaceborne microwave remote sensing systems for ocean windspeed measurement, ocean wave imaging and sea ice studies was demonstrated. Development of large space antennas offers some interesting possibilities for rain rate measurements, ocean and ice studies, and radar imaging. The joint use of active and passive sensors using the 15 m antenna for ocean, ice, and soil moisture studies; rain rate measurements; and radar imaging is considered. Verification of the frequency agile rain radar concept with Shuttle offers the possibility of much needed rain rate statistics over the ocean

    Comments on the nature of the outside boundary layer on a liquid sphere in a steady, uniform stream

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    Nature of outside boundary layer on liquid sphere in steady, uniform strea

    Draft genome sequences of three newly identified species in the genus Cronobacter, C. helveticus LMG23732T, C. pulveris LMG24059, and C. zurichensis LMG23730T

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    Cronobacter helveticus, Cronobacter pulveris, and Cronobacter zurichensis are newly described species in the Cronobacter genus, which is associated with serious infections of neonates. This is the first report of draft genome sequences for these species

    Draft genome sequence of the earliest Cronobacter sakazakii sequence type 4 strain, NCIMB 8272

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    The Cronobacter sakazakii clonal lineage defined as sequence type 4 (ST4) is associated with severe cases of neonatal meningitis and persistence in powdered infant formula. For genome sequencing of the earliest deposited culture collection strain of Cronobacter sakazakii ST4, we used the strain NCIMB 8272, originally isolated from milk powder in 1950

    Genomic dissection of the 1994 Cronobacter sakazakii outbreak in a French neonatal intensive care unit

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    Background: Cronobacter sakazakii is a member of the genus Cronobacter that has frequently been isolated from powdered infant formula (PIF) and linked with rare but fatal neonatal infections such as meningitis and necrotising enterocolitis. The Cronobacter MLST scheme has reported over 400 sequence types and 42 clonal complexes; however C. sakazakii clonal complex 4 (CC4) has been linked strongly with neonatal infections, especially meningitis. There have been a number of reported Cronobacter outbreaks over the last three decades. The largest outbreak of C. sakazakii was in a neonatal intensive care unit (NICU) in France (1994) that lasted over 3 months and claimed the lives of three neonates. The present study used whole genome sequencing data of 26 isolates obtained from this outbreak to reveal their relatedness. This study is first of its kind to use whole genome sequencing data to analyse a Cronobacter outbreak. Methods: Whole genome sequencing data was generated for 26 C. sakazakii isolates on the Illumina MiSeq platform. The whole genome phylogeny was determined using Mugsy and RaxML. SNP calls were determined using SMALT and SAMtools, and filtered using VCFtools. Results: The whole genome phylogeny suggested 3 distant clusters of C. sakazakii isolates were associated with the outbreak. SNP typing and phylogeny indicate the source of the C. sakazakii could have been from extrinsic contamination of reconstituted infant formula from the NICU environment and personnel. This pool of strains would have contributed to the prolonged duration of the outbreak, which was up to 3 months. Furthermore 3 neonates were co-infected with C. sakazakii from two different genotype clusters. Conclusion: The genomic investigation revealed the outbreak consisted of an heterogeneous population of C. sakazakii isolates. The source of the outbreak was not identified, but probably was due to environmental and personnel reservoirs resulting in extrinsic contamination of the neonatal feeds. It also indicated that C. sakazakii isolates from different genotype clusters have the ability to co-infect neonates

    Investigation of radar backscattering from second-year sea ice

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    The scattering properties of second-year ice were studied in an experiment at Mould Bay in April 1983. Radar backscattering measurements were made at frequencies of 5.2, 9.6, 13.6, and 16.6 GHz for vertical polarization, horizontal polarization and cross polarizations, with incidence angles ranging from 15 to 70 deg. The results indicate that the second-year ice scattering characteristics were different from first-year ice and also different from multiyear ice. The fading properties of radar signals were studied and compared with experimental data. The influence of snow cover on sea ice can be evaluated by accounting for the increase in the number of independent samples from snow volume with respect to that for bare ice surface. A technique for calculating the snow depth was established by this principle and a reasonable agreement has been observed. It appears that this is a usable way to measure depth in snow or other snow-like media using radar
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