Components required for in vitro cleavage and polyadenylation of eukaryotic mRNA

We have studied in vitro cleavage/polyadenylation of precursor RNA containing herpes simplex virus type 2 poly A site sequences and have analysed four RNA/protein complexes which form during in vitro reactions. Two complexes, A and B, form extremely rapidly and are then progressively replaced by a third complex, C which is produced following cleavage and polyadenylation of precursor RNA. Substitution of ATP with cordycepin triphosphate prevents polyadenylation and the formation of complex C however a fourth complex, D, results which contains cleaved RNA. A precursor RNA lacking GU-rich downstream sequences required for efficient cleavage/ polyadenylation fails to form complex B and produces a markedly reduced amount of complex A. As these GU-rich sequences are required for efficient cleavage, this establishes a relationship between complex B formation and cleavage/polyadenylation of precursor RNA in vitro. The components required for in vitro RNA processing have been separated by fractionation of the nuclear extract on Q-Sepharose and Biorex 70 columns. A Q-Sepharose fraction forms complex B but does not process RNA. Addition of a Biorex 70 fraction restores cleavage activity at the poly A site but this fraction does not appear to contribute to complex formation. Moreover, in the absence of polyethylene glycol, precursor RNA is not cleaved and polyadenylated, however, complexes A and B readily form. Thus, while complex B is necessary for in vitro cleavage and polyadenylation, it may not contain all the components required for this processing

Raman Characterization Studies Of Synthetic And Natural Mgal 2o4 Crystals

Raman studies are reported for one natural and four synthetic MgAl 2O4 spinel crystals and symmetry assignments for the phonon modes of the spinel crystal structure are given. Deviations in the Raman selection rules are observed for the synthetic spinel crystals in the form of additional modes over and above those theoretically predicted. Also, detailed studies of one of the synthetic crystals show a probable disordering in the Mg-Al sites. Raman linewidths and violations of selection rules, as reported here, can aid in the characterization and quality control of synthetically grown crystals.5893585359

5D Black Rings and 4D Black Holes

It has recently been shown that the M theory lift of a IIA 4D BPS Calabi-Yau black hole is a 5D BPS black hole spinning at the center of a Taub-NUT-flux geometries, and a certain linear relation between 4D and 5D BPS partition functions was accordingly proposed. In the present work we fortify and enrich this proposal by showing that the M-theory lift of the general 4D multi-black hole geometry are 5D black rings in a Taub-NUT-flux geometry.Comment: 8 pages; version 2, with additional references and explanation

Monitoring Handbook 6: Analyzing and interpreting monitoring data

The basic purpose of data analysis is to identify patterns of change in your indicator over time, and to evaluate these changes. Without doing some kind of analysis, it will be difficult for you to know the effect your project is actually having. The data analysis techniques presented in this handbook are not difficult. Most of them can be easily done using little more than a calculator and scratch paper. If necessary, there are resources listed in the handbook for additional assistance analyzing your data

Optimizing wide-area sound reproduction using a single subwoofer with dynamic signal decorrelation

A central goal in small room sound reproduction is achieving consistent sound energy distribution across a wide listening area. This is especially difficult at low-frequencies where room-modes result in highly position-dependent listening experiences. While numerous techniques for multiple-degree-of-freedom systems exist and have proven to be highly effective, this work focuses on achieving position-independent low-frequency listening experiences with a single subwoofer. The negative effects due to room-modes and comb-filtering are mitigated by applying a time-varying decorrelation method known as dynamic diffuse signal processing. Results indicate that spatial variance in magnitude response can be significantly reduced, although there is a sharp trade-off between the algorithm’s effectiveness and the resulting perceptual coloration of the audio signal.N/