MicroRNA-30c als Biomarker im Liquor cerebrospinalis zur Unterscheidung zwischen primären und sekundären Lymphomen des zentralen Nervensystems

Primäre und sekundäre ZNS-Lymphome differieren in Prognose und Therapie, können histologisch allerdings nicht voneinander unterschieden werden. Die miRNA-Expression im Liquor cerebrospinalis von Patienten mit einem primären und sekundären ZNS-Lymphom wurde mittels miRNA-Arrays untersucht und die miRNA mit der am stärksten differenzierten Expression, miR-30c, an einer größeren Kohorte validiert. Mittels eines $\textit {in silico}$ Zielgenvorhersage-Algorithmus ("miRanda") wurde ein potentielles Zielgen für miR-30c ermittelt. MiR-30c wies ein signifikant differentes Expressionsverhalten in beiden Gruppen auf und zeigte im Liquor cerebrospinalis von Pat. mit SZNSL signifikant höhere Werte als bei PZNSL-Patienten (p < 0.001). Die Sensitivität und Spezifität von miR-30c als diagnostischer Biomarker wurde mit 92,7 % bzw. 83,7 % bestimmt. Die Ergebnisse suggerieren das Potential von miR-30c als Biomarker im Liquor für die Unterscheidung zwischen primären und sekundären ZNS- Lymphomen

Shear-Induced Molecular Orientation of Compression Moulded PE-HD Sheets

The article presents experimental results on orientation of polymer macromolecules in compression moulded high density polyethylene sheets. Properties anisotropy of thermoplastic films or sheets, that are usually formed in the extrusion process, causes deformation of thermoformed products and is a serious technological problem. One of the possible solutions of this problem is application of compression moulded sheets. The paper presents the results of tests of selected strength characteristics of compression moulded PE-HD sheets. A static tensile test was performed and Charpy impact strength was determined. Additionally Chrystler’s orientation test was executed. For comparison the same experiments were performed on extruded sheets. Samples were cut in directions perpendicular and parallel to the direction of polymer flow in pressing and extrusion processes. The obtained results indicate that the compression moulding technique allows the production of sheets that do not exhibit statistically significant anisotropy of the tested strength properties

Prediction of Strength Properties of Filling Packets in Selected Cooling Towers

The operating conditions of thermoplastic polymer materials determine the changes in their functional properties. Accelerated aging tests do not give a full picture of the changes taking place in the polymer material, hence the conclusions drawn on the basis of exposure of these materials to damaging effects in real operating conditions are particularly important. The aim of the study was to determine the degree of degradation of polypropylene films used in the drainage blocks of cooling towers in a selected power plant in the Silesian voivodship, which allowed forecasting the operating time over a period of 10 years. A number of 600 mm high drip blocks were tested, on which 300 mm high blocks were mounted. The tests were carried out on films subjected to the aging process in the conditions of continuous operation of a cooling tower (almost 100% humidity). The water flow is accompanied by heat exchange, the side effect of which is deposits formation on the surface of the drip blocks, negatively affecting the operation of the cooling tower. The degree of degradation resulting from operational aging was assessed on the basis of the strength properties determined in the static tensile test, thermogravimetric analysis and FTIR spectra. Changes in properties during operation were determined on the basis of the obtained results of the strength tests, which were compared with the tensile strength and elongation at break of reference samples (not subjected to aging in the operating conditions of cooling tower drip blocks). The obtained results were related to the properties of the reference samples not subjected to the degradation process. Based on the collected data, the tensile strength and deformation at fracture after a 10-year service life were predicted

Durability and Corrosion Resistance Test of Adhesive Joints Using Two Adherence Promoters for the Connection of Aerospace Aluminum Alloys

This article presents the technology of making an adhesive joint using two primers: Corrosion Inhibiting Primer BR127 (previously used, containing chromium compounds) and, as a potential substitute, Structural Adhesives Primer EW 5000 AS (which does not contain any compounds harmful to the environment). An adhesive film and a sol&ndash;gel primer were used to make the joint of two aluminum sheets, and various technologies were used for applying adhesion promoters. The mechanical properties of the prepared samples were tested using two test methods: wedge tests and shear strength tests. In both cases, the samples were aged in laboratory conditions in tap water, and in a climatic chamber (with increased temperature and humidity). The obtained results indicate that the best technology for preparing the joint using each primer is the technology that assumes heating the primer and hardening the adhesive film in one operation. The results of the strength tests indicate that the samples made using the EW 5000 AS primer have higher strength properties under all tested seasoning conditions compared to samples made using the BR 127 primer. It was also confirmed that the presence of moisture and/or water reduces the mechanical strength of the adhesive joints independently of the primer used. The results of the polymer coatings tests to protect the aluminum substrate against corrosion showed that the coatings are only effective for a certain period of time, and, as a result of the NSS test, after 480 h, all the samples were subject to corrosion

Wpływ przyspieszonego starzenia na wybrane właściwości mechaniczne polipropylenu z napełniaczami organicznymi

The aging effect in water atthe temperature of 100±2°C on selected mechanical properties of polypropylene with organic fillers (hemp chaff, a mixture of oak, birch and maple leaves) was investigated. A significant influence of the aging process on the impact strength and elongation at break was observed. The degradation processes were also visible on the surface of the samples.Zbadano wpływ starzenia w wodzie w temperaturze 100±2°C na wybrane właściwości mechaniczne polipropylenu z napełniaczami organicznymi (plewy konopne, mieszanina liści dębu, brzozy i klonu). Stwierdzono istotny wpływ procesu starzenia na udarność i wydłużenie względne przy zerwaniu. Procesy degradacji widoczne były również na powierzchni próbek

Proteomic analysis of the extracellular matrix in idiopathic pes equinovarus

Abstract Idiopathic pes equinovarus is a congenital deformity of the foot and lower leg defined as a fixation of the foot in adduction, supination, and varus. Although the pathogenesis of clubfoot remains unclear, it has been suggested that fibroblasts and growth factors are involved. To directly analyze the protein composition of the extracellular matrix in contracted tissue of patients with clubfoot. A total of 13 infants with idiopathic clubfoot treated with the Ponseti method were included in the present study. Tissue samples were obtained from patients undergoing surgery for relapsed clubfeet. Contracted tissues were obtained from the medial aspect of the talonavicular joint. Protein was extracted after digestion and delipidation using zip-tip C18. Individual collagenous fractions were detected using a chemiluminescent assay. Amino acid analysis of tissue samples revealed a predominance of collagens, namely collagen types I, III, and VI. The high content of glycine and h-proline suggests a predominance of collagens I and III. A total of 19 extracellular matrix proteins were identified. The major result of the present study was the observation that the extracellular matrix in clubfoot is composed of an additional 16 proteins, including collagens V, VI, and XII, as well as the previously described collagen types I and III and transforming growth factor b. The characterization of the general protein composition of the extracellular matrix in various regions of clubfoot may help in understanding the pathogenesis of this anomaly and, thus, contribute to the development of more efficacious therapeutic approaches