6 research outputs found
Receptor-Receptor Coupling in Bacterial Chemotaxis: Evidence for Strongly Coupled Clusters
AbstractReceptor coupling is believed to explain the high sensitivity of the Escherichia coli chemotaxis network to small changes in levels of chemoattractant. We compare in detail the activity response of coupled two-state receptors for different models of receptor coupling: weakly-coupled extended one-dimensional and two-dimensional lattice models and the Monod-Wyman-Changeux model of isolated strongly-coupled clusters. We identify features in recent data that distinguish between the models. Specifically, researchers have measured the receptor activity response to steps of chemoattractant for a variety of engineered E. coli strains using in vivo fluorescence resonance energy transfer. We find that the fluorescence resonance energy transfer results for wild-type and for a low-activity mutant are inconsistent with the lattice models of receptor coupling, but consistent with the Monod-Wyman-Changeux model of receptor coupling, suggesting that receptors form isolated strongly-coupled clusters
Black Hole-Neutron Star Binaries in General Relativity: Quasiequilibrium Formulation
We present a new numerical method for the construction of quasiequilibrium
models of black hole-neutron star binaries. We solve the constraint equations
of general relativity, decomposed in the conformal thin-sandwich formalism,
together with the Euler equation for the neutron star matter. We take the
system to be stationary in a corotating frame and thereby assume the presence
of a helical Killing vector. We solve these coupled equations in the background
metric of a Kerr-Schild black hole, which accounts for the neutron star's black
hole companion. In this paper we adopt a polytropic equation of state for the
neutron star matter and assume large black hole--to--neutron star mass ratios.
These simplifications allow us to focus on the construction of quasiequilibrium
neutron star models in the presence of strong-field, black hole companions. We
summarize the results of several code tests, compare with Newtonian models, and
locate the onset of tidal disruption in a fully relativistic framework.Comment: 17 pages, 7 figures; added discussion, tables; PRD in pres
Comparing Criteria for Circular Orbits in General Relativity
We study a simple analytic solution to Einstein's field equations describing
a thin spherical shell consisting of collisionless particles in circular orbit.
We then apply two independent criteria for the identification of circular
orbits, which have recently been used in the numerical construction of binary
black hole solutions, and find that both yield equivalent results. Our
calculation illustrates these two criteria in a particularly transparent
framework and provides further evidence that the deviations found in those
numerical binary black hole solutions are not caused by the different criteria
for circular orbits.Comment: 4 pages; to appear in PRD as a Brief Report; added and corrected
reference
Activated membrane patches guide chemotactic cell motility.
Many eukaryotic cells are able to crawl on surfaces and guide their motility based on environmental cues. These cues are interpreted by signaling systems which couple to cell mechanics; indeed membrane protrusions in crawling cells are often accompanied by activated membrane patches, which are localized areas of increased concentration of one or more signaling components. To determine how these patches are related to cell motion, we examine the spatial localization of RasGTP in chemotaxing Dictyostelium discoideum cells under conditions where the vertical extent of the cell was restricted. Quantitative analyses of the data reveal a high degree of spatial correlation between patches of activated Ras and membrane protrusions. Based on these findings, we formulate a model for amoeboid cell motion that consists of two coupled modules. The first module utilizes a recently developed two-component reaction diffusion model that generates transient and localized areas of elevated concentration of one of the components along the membrane. The activated patches determine the location of membrane protrusions (and overall cell motion) that are computed in the second module, which also takes into account the cortical tension and the availability of protrusion resources. We show that our model is able to produce realistic amoeboid-like motion and that our numerical results are consistent with experimentally observed pseudopod dynamics. Specifically, we show that the commonly observed splitting of pseudopods can result directly from the dynamics of the signaling patches