REQUIRED COEFFICIENT OF FRICTION ANALYSES IN RUNNING

The purpose of this study was to analyze the possible alterations in the required coefficient of friction (RCOF) in running under the following conditions: a) barefoot against shod; b) self-selected velocity and cadence versus imposed cadence and c) along three running phases (initial contact, mid-stance and propulsion). Two Kistler force plates were used to measure the horizontal and vertical components of ground reaction forces in order to calculate the RCOF. Statistical differences were found for cadence and phase factors. Barefoot-Shod conditions did not present statistical differences. An interaction between velocity and phase of cycle was found. At propulsion phase, an increased RCOF were revealed, especially with the interaction of an imposed cadence. In conclusion, the present study supports the relevance of RCOF as a variable affecting and being affected during running to be taken into consideration at many experimental conditions

Invasion of <it>Ureaplasma diversum </it>in Hep-2 cells

<p>Abstract</p> <p>Background</p> <p>Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the <it>Ureaplasma diversum </it>mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Microscopy and gentamicin invasion assay.</p> <p>Results</p> <p>The isolates and strains used were detected inside the cells after infection of one minute without difference in the arrangement for adhesion and invasion. The adhesion was scattered throughout the cells, and after three hours, the invasion of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% of the ATCC strains were inside the infected Hep-2 cells in contrast to 10% to the clinical isolates. A high level of phospholipase C activity was also detected in all studied ureaplasma.</p> <p>Conclusions</p> <p>The results presented herein will help better understand <it>U. diversum </it>infections, aswell as cellular attachment and virulence.</p