The human immune response to the flagellins of Burkholderia dolosa.

Cystic Fibrosis (CF) is the most commonly inherited lethal disorder in Caucasian populations. Chronic bacterial infection both decreases lung function and lowers the quality of the CF patient’s life. In one of the most recent CF epidemics, Burkholderia dolosa, an organism previously uncharacterized as an epidemic strain, infected over forty patients and led to the death of over seven patients at Children’s Hospital Boston. Preliminary research revealed that this strain of B. dolosa possesses genes for two distinct types of flagella, which is uncommon in bacterial pathogens. In this study we examine the interaction of human cells and B. dolosa, focusing on the human inflammatory response to these two types of B. dolosa flagella. The findings of this study could provide insight into B. dolosa pathogenesis, so that effective therapeutics may be designed to combat this organism in the future

A Measure Development Study for Youth Trauma Exposure and Developmental Trauma Disorder

Childhood trauma research and assessment are limited in addressing complex trauma. Specifically, current childhood trauma exposure measures are limited in the types of trauma queried, the ability to assess for frequency of trauma incidents, and the possibility of reporting on symptoms from multiple traumas (Hawkins & Radcliffe, 2006). Another problem with current childhood trauma practices is related to diagnosis. Most children who experience complex trauma are not diagnosed with PTSD; separation anxiety disorder and oppositional defiant disorder are most commonly diagnosed (Cook et al., 2005). Emotional and behavioral difficulties associated with complex trauma in childhood may be better captured by symptoms of a proposed diagnosis, developmental trauma disorder (DTD; van der Kolk, 2005). This measure development study included testing of an improved childhood trauma exposure measure, the Stressful Events Questionnaire (SEQ), and a measure to assess for DTD symptoms, the Developmental Trauma Disorder Questionnaire (DTDQ) in a clinical child sample. Children exposed to a variety of trauma experiences were assessed utilizing the SEQ, which includes potentially traumatic experiences and assesses for frequency of incidents, as well as utilizing the DTDQ. Results provided preliminary support for the reliability and validity of the SEQ and DTDQ in a clinical child sample. Results also provided empirical support for a broadened PTSD criterion A and support for DTD criteria. This study has implications for the diagnosis and treatment of trauma experiences in youth