Forschungsschwerpunkt. Grundlagen und Anwendungen der Gentechnologie Abschlussbericht 1992-1995

SIGLEAvailable from TIB Hannover: F96B1937+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Grundlagen und Anwendungen der Gentechnologie Jahresbericht 1990

SIGLEAvailable from TIB Hannover: ZO 5875(1990) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Grundlagen und Anwendungen der Gentechnologie Abschlussbericht 1984-1991

SIGLEAvailable from TIB Hannover: FR 7046 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Forschungsschwerpunkt 'Neue Faktoren und Mechanismen der Genexpression' Abschlussbericht

SIGLEAvailable from TIB Hannover: F99B1706+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman

Molekularbiologische Methoden fuer Medizin und Technologie Abschlussbericht 1990-1993

SIGLEAvailable from TIB Hannover: ZO 7867(1990/93) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

Molekularbiologie von Pertussis Toxin - Kartierung immunogener und antigener Determinanten der S1 Untereinheit, Eingrenzung funktioneller Domaenen und Entwicklung einer einfachen Immundiagnose fuer B.pertussis Infektionen Schlussbericht

Pertussis toxin secreted by virulent B. pertussis organisms is one of the major virulence factors and as such an essential component of the present and of any newly developed cellular or acellular pertussis vaccine. PT is built according to the A-B model and consists of the immunodominant S1 subunit (ADP ribosyl transferase) and of the B-oligomer consisting of the subunits S2, S3, 2 x S4, and S5. The B-oligomer is responsible for receptor recognition and possibly also for translocation of S1. In this project the NAD-binding site(s) in S1 have been mapped. Glu-129 has been identified by photoaffinity as an esential amino acid for NAD binding. Site-directed mutagenesis with an exchange of four amino acids by AEC-PCR led to the total inactivation of PT. Regions responsible for the NAD-glycohydrolase and the ADP-ribosyltransferase activities of S1 were identified and further characterized. Also, the C-terminal region necessary for binding to acceptor G-proteins was elucidated. With the help of a 'pepscan' analysis a linear C-terminal determinant was identified which was found to be indicative of acute pertussis infections and thus might be helpful for the development of a new differential diagnostic method for acute pertussis. Immunogenic and antigenic determinants of S1 have been further characterized with the help of synthetic peptides and with the expression of partial sequences in an fd-phage expression system. Linear determinants responsible for receptor recognition the S3 subunit were elucidated. Antibodies raised against these determinants and the corresponding peptide-conjugates were able to inhibit the activity of PT in tissue culture. All subunits have been expressed in E.coli. The recombinant subunits could be partially reconstituted to the dimers of the B-oligomer. (orig.)Available from TIB Hannover: DtF QN1(27,18) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

Funktion des menschlichen Myc-Proteins Abschlussbericht

Available from TIB Hannover: DtF QN1(48,14) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Molekulare Mechanismen und Genetik der Gliazelldifferenzierung. Teilprojekt C8 Abschlussbericht

Available from TIB Hannover: DtF QN1(48,13) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman