Comparison of structures and cytotoxicity of mupirocin and batumin against melanoma and several other cancer cell lines

AIM : To determine the computer-predicted anticancer activity of mupirocin and to compare its activities with those determined for another polyene antibiotic, batumin. MATERIALS AND METHODS : Molecular docking, cytotoxicity assays, cell microscopy and cell cycle progression were studied in cancer and nontumorigenic cell lines. RESULTS AND CONCLUSION : Cytotoxicity of mupirocin against several cancerous cell lines was detected with the highest one (IC50 = 5.4 μg/ml) against melanoma cell line. The profile of cytotoxicity of mupirocin was similar to that reported for batumin. Nevertheless, the morphology of cells treated with these antibiotics and alterations in cell cycle progression suggested possible dissimilarity in their mechanisms of action. Selective cytotoxicity of mupirocin against melanoma cells potentiates further studies to discover nontoxic drugs for melanoma prevention.AM Joubert acquired grants from the National Research Foundation (NRF; 105992, 90523 and 85818), Cancer Association of South Africa (A0V741 and A0W228), the Struwig Germeshuysen Trust (A0N074), the School of Medicine Research Committee of the University of Pretoria (AOH561) and Medical Research Council (A0W110). MH Visagie was funded from the NRF (99706), the School of Medicine Research Committee of the University of Pretoria (AOH561) and Struwig Germeshysen Trust. N Lall was funded from NRF/IKS160514165042 Grant No: 105169. ON Reva was funded from NRF grant 105996.https://www.future-science.com/journal/fmchj2019BiochemistryGeneticsMicrobiology and Plant PathologyPhysiologyPlant Production and Soil Scienc

Promising anticancer activity of batumin : a natural polyene antibiotic produced by Pseudomonas batumici

AIM : To determine the computer-predicted anticancer activity of antibiotic batumin. MATERIALS AND METHODS : Cytotoxicity assays, cell morphology microscopy and cell cycle progression were studied in cancer and nontumorigenic cell lines. An in vivo experiment on Lewis lung carcinoma (3LL)-transplanted mice was conducted to evaluate potential antimetastatic. RESULTS AND CONCLUSION : Cytotoxicity against melanoma and lung carcinoma cells (IC50 ≈ 5 μg/ml) was detected. Hypercondensed chromatin and apoptotic body formation in batumin-treated cells suggested the induction of apoptosis supported also by an observed increase in the quantity of cells occupying the sub-G1 cell cycle phase. Twofold reduction in the number and volume of lung metastases in Lewis lung carcinoma (3LL)-bearing batumin-treated mice was demonstrated. Highly specific cytotoxicity of batumin against cancer cell lines potentiates further studies.https://www.future-science.com/loi/fmc2019-09-01hj2018BiochemistryGeneticsMicrobiology and Plant PathologyPhysiologyPlant Production and Soil Scienc