48 research outputs found
Microbial Delivery Vehicles for Allergens and Allergen-Derived Peptides in Immunotherapy of Allergic Diseases
Allergen-specific immunotherapy represents the only available curative approach to allergic diseases. The treatment has proven effective, but it requires repetitive administrations of allergen extracts over 3ā5 years and is often associated with adverse events. This implies the need for novel therapeutic strategies with reduced side effects and decreased treatment time, which would improve patientsā compliance. Development of vaccines that are molecularly well defined and have improved safety profile in comparison to whole allergen extracts represents a promising approach. Molecular allergy vaccines are based on major allergen proteins or allergen-derived peptides. Often, such vaccines are associated with lower immunogenicity and stability and therefore require an appropriate delivery vehicle. In this respect, viruses, bacteria, and their protein components have been intensively studied for their adjuvant capacity. This article provides an overview of the microbial delivery vehicles that have been tested for use in allergy immunotherapy. We review in vitro and in vivo data on the immunomodulatory capacity of different microbial vehicles for allergens and allergen-derived peptides and evaluate their potential in development of allergy vaccines. We also discuss relevant aspects and challenges concerning the use of microbes and their components in immunotherapy of allergic diseases
Cardiotoxic activity of polymeric 3-alkylpyridinium salts from the marine sponge Reniera sarai
Associations among different functional and structural arterial wall properties and their relations to traditional cardiovascular risk factors in healthy subjects: a cross-sectional study
BACKGROUND: The arterial wall possesses several functional and structural properties that define arterial health. Once they become impaired, cardiovascular risk increases. We aimed to ascertain the pattern of correlations among different arterial wall properties and to explore their relations to traditional risk factors and cardiovascular risk stratification. To allow such an investigation a middle-aged healthy population was recruited. METHODS: This cross-sectional study included 100 healthy males (aged 41.9 Ā± 6.4 years). Pulse wave velocity (PWV), Ī²-stiffness and intima-media thickness (IMT) of the carotid artery, and brachial artery flow-mediated dilation (FMD) were measured by a standardized ultrasound approach. RESULTS: No correlation between FMD and IMT was found; only relatively poor correlations between PWV (or Ī²-stiffness) and FMD existed, as well as between PWV (or Ī²-stiffness) and IMT. PWV and Ī²-stiffness highly correlated. Unexpectedly, only weak associations between PWV, Ī²-stiffness, FMD, IMT and traditional risk factors were revealed. Hence, traditional risk factors (mainly age) explained only 10-50% of variability for PWV, Ī²-stiffness, FMD and IMT. Although the subjects had low cardiovascular risk according to their Framingham score, their arterial wall properties were already impaired, particularly FMD. CONCLUSIONS: In healthy middle-age males we found: i) absent or poor correlations among arterial stiffness, IMT and endothelial function; ii) a low impact of traditional risk factors on the studied variables, and iii) the presence of impaired arterial wall properties despite low calculated cardiovascular risk. These results provide a deepened understanding of arterial wall properties and could help to improve cardiovascular risk stratification
DiabetiÄna ketoacidoza: redek, a resen zaplet zdravljenja z zaviralci SGLT-2 ā pregled literature in prikaz serije primerov
Zaviralci natrij-glukoznega koprenaÅ”alnega sistema-2 (SGLT-2) se vse pogosteje predpisujejo tako v diabetologiji kot tudi v drugih internistiÄnih strokah. Primarno ugodno vplivajo na presnovne dejavnike, tj. parametre glikemije in na telesno maso. Poleg tega omogoÄajo zaÅ”Äito tarÄnih organov, srÄno-žilnega sistema in ledvic, pri osebah s sladkorno boleznijo ali brez nje. Neželeni uÄinki ob zdravljenju z zaviralci SGLT-2 so redki, veÄinoma nenevarni in jih lahko prepreÄimo. Zelo redek, a življenje ogrožajoÄ zaplet je lahko pojav diabetiÄne ketoacidoze. Dejavnikov, ki poveÄajo tveganje za njen nastanek, Å”e niso popolnoma raziskali. V prispevku so opisani diabetiÄna ketoacidoza in njene posebnosti ob zdravljenju z zaviralci SGLT-2 ter možni vzroÄni dejavniki zanjo. Poznavanje slednjih je predvsem pomembno zaradi možnosti aktivnega prepreÄevanja diabetiÄne ketoacidoze. Najpogosteje se pojavi hkrati z zdravljenjem z zaviralci SGLT-2 ob okužbah, sladkorni bolezni tipa 1, opustitvi inzulinskega zdravljenja ter v primerih stradanja. Prispevek vkljuÄuje tudi prikaz serije primerov, ki so bili hospitalizirani na naÅ”em kliniÄnem oddelku zaradi diabetiÄne ketoacidoze ob zdravljenju z zaviralci SGLT-2, ob razliÄni soÄasni antidiabetiÄni terapiji
Cardioprotective effects of bilberry extract on ischemia-reperfusion-induced injury in isolated rat heart
The preventive cardiovascular effect of a combination of statin and angiotensin receptor blocker at sub-therapeutic doses in middle-aged healthy volunteers
Thorough Clinical Evaluation of Skin, as well as Oral, Genital and Anal Mucosa is Beneficial in Lichen Planus Patients
Lichen planus (LP) is a common mucocutaneous disease of unknown aetiology with various geographical prevalence,
may be related to some serious disorders such as squamous cell carcinoma and often remains underdiagnosed. The aim
of this retrospective study was to thoroughly determine localization and clinical characteristics of LP lesions in a cohort
of 173 Slovenian patients in association to the presence of accompanying symptoms and history of potential stressful
events. Isolated cutaneous lesions of LP were found in 56.6% and isolated oral LP in 3.5% of patients. Thirty-four percent
presented orocutaneous LP, whereas genitocutaneous LP was noted in 1.2%, orogenito-cutaneous LP in 4% and
orogenital LP in 0.5% of patients. Underlying stressful events were noted in 36 out of 137 (26.3%) patients. Despite obviously
visible localization of the lesions various medical specialists should be familiar with LP and thoroughly examine
the complete skin, as well as oral, genital and anal mucosa in each LP patient to avoid a delay in diagnosing this disease
and possibly disclose a much serious underlying condition. Psychological support should be offered, if needed
Thorough Clinical Evaluation of Skin, as well as Oral, Genital and Anal Mucosa is Beneficial in Lichen Planus Patients
Lichen planus (LP) is a common mucocutaneous disease of unknown aetiology with various geographical prevalence,
may be related to some serious disorders such as squamous cell carcinoma and often remains underdiagnosed. The aim
of this retrospective study was to thoroughly determine localization and clinical characteristics of LP lesions in a cohort
of 173 Slovenian patients in association to the presence of accompanying symptoms and history of potential stressful
events. Isolated cutaneous lesions of LP were found in 56.6% and isolated oral LP in 3.5% of patients. Thirty-four percent
presented orocutaneous LP, whereas genitocutaneous LP was noted in 1.2%, orogenito-cutaneous LP in 4% and
orogenital LP in 0.5% of patients. Underlying stressful events were noted in 36 out of 137 (26.3%) patients. Despite obviously
visible localization of the lesions various medical specialists should be familiar with LP and thoroughly examine
the complete skin, as well as oral, genital and anal mucosa in each LP patient to avoid a delay in diagnosing this disease
and possibly disclose a much serious underlying condition. Psychological support should be offered, if needed
Problematika uživanja pijaÄ z dodanim sladkorjem v Sloveniji in svetu
Pojavnost kardiometabolnih bolezni je dosegla pandemiÄne razsežnosti, zato se Äedalje veÄja pozornost posveÄa tudi z življenjskim slogom pogojenemu zdravju prebivalstva, Å”e posebej naÄinu prehranjevanja in vnosu doloÄenih skupin živil. Å tevilne epidemioloÅ”ke raziskave so dokazale, da je uživanje pijaÄ z dodanim sladkorjem povezano z nastankom debelosti, sladkorne bolezni in srÄno-žilnih bolezni, dodatno je tudi dober pokazatelj posameznikovega nezdravega življenjskega sloga. PijaÄe z dodanim sladkorjem so vir tako imenovanih Ā»praznih kalorijĀ«, ki nimajo hranilne vrednosti in predstavljajo najveÄji dodatni vir energije in vnosa dodanih sladkorjev, Å”e posebej fruktoze. ZmanjÅ”anje vnosa pijaÄ z dodanim sladkorjem dokazano vodi v znižanje telesne mase in zmanjÅ”anje tveganja za nastanek kardiometabolnih bolezni. Uživanje pijaÄ z dodanim sladkorjem je uvrÅ”Äeno kar med 15 najpogostejÅ”ih dejavnikov tveganja med kazalci nezdravega življenjskega sloga. PoslediÄno je smiselno intenzivno ozaveÅ”Äanje o pomembnosti opustitve uživanja pijaÄ z dodanim sladkorjem, Å”e posebej pri osebah s poveÄanim tveganjem za presnovne bolezni in mladih z nezdravim življenjskim slogom. V prispevku so opredeljene pijaÄe z dodanim sladkorjem, opisana je njihova povezava z debelostjo, sladkorno boleznijo in srÄno-žilnimi boleznimi ter možni zdravi nadomestki
Zaviralci SGLT-2
Zaviralci natrij-glukoznega prenaÅ”alnega sistema 2 (SGLT-2) so najnovejÅ”a skupina zdravil za zdravljenje sladkorne bolezni tipa 2, ki so v kliniÄni uporabi zadnjih nekaj let. V proksimalnih tubulih ledvic zmanjÅ”ajo reabsorpcijo glukoze, ki se zato izloÄi z urinom. Zato se zniža koncentracija glukoze v krvi in nastopi ugodni vpliv na urejenost glikemije, obenem pa s tem ne poveÄajo tveganja za hipoglikemijo. Zaviralci SGLT-2 imajo tudi ugodne presnovne uÄinke, in sicer znižajo telesno maso, krvni tlak in koncentracijo seÄne kisline v serumu. UpoÄasnijo tudi napredovanje zaÄetne diabetiÄne ledviÄne bolezni. Za nekatere predstavnike so tudi pokazali, da pomembno zmanjÅ”ujejo pojavnost srÄno-žilnih dogodkov in zapletov, vendar mehanizem Å”e ni v celoti raziskan in je predmet intenzivnega preuÄevanja. Redko povzroÄijo resne neželene uÄinke. V prispevku je opisan mehanizem delovanja zaviralcev SGLT-2, njihov vpliv na urejenost glikemije in drugo: presnovni uÄinki, vpliv na pojavnost srÄno-žilnih bolezni in najpogostejÅ”i neželeni uÄinki ter možnosti predpisovanja zaviralcev SGLT-2 v Sloveniji