Identification of human blood by fibrin plate method, a supplemental study

Following Fibrin Plate Method of SZOLLOSY and RENGEI² , and ASTRUP and MULLERTZ³, the author conducted a series of experiments in an attempt to identify human blood by detecting the proactivator believed to be one of the enzyme proteins contained abundantly in human blood. As the results it has been found that with 0.1 mg. % SK-solution human blood alone responds to the reaction, showing almost absolute species-specificity within 4 hours but not with blood of monkey. In addition, the sensitivity is so high that it responds positively up to the dilution of 1: 8,000 to 1: 10,000 (human blood: physiological saline solution). By means of this method using 0.1 mg% SK-solution it has been clearly demonstated that the identification of human blood is possible in a variety of conditions and states as may be encountered in practical legal medicine such as with blood stains in cloth, wood, stone, leaves of tree even with a trace of blood stain, old human blood stain left standing for 20 to 30 years, old blood mixed with iron rust, blood stains soaked in various oils, and even the blood stained cloth washed thoroughly and left standing in room temperature for 6 months. Therefore, this Fibrin Plate Method seems to be the excellent one for the identification of human blood.</p

Post mortem activation of human blood fibrinolytic enzyme in sudden and natural deaths

With the purpose to elucidate the cause and difference of blood fluidity in sudden death and natural one, we have observed the fibrinolysis of the blood in medico-legal and pathological autopsies by means of Fibrin Plate Method, a routine method devised in our laboratory. As the result it has been found that in the blood serum of sudden death and in some of natural deaths from tumors, leukemias, etc., the decrease in fibrinolytic activity is equivalent to the amount of proactivator that combined with the SK-like substance liberated into blood. On the other hand, in the blood of most of natural deaths, and in that bled from vessels and stored in body cavities, no natural fibrinolysis is observable and the same fibrinolytic activity with SK as normal one is demonstrated. Thus it is concluded that the cause of blood fluidity in sudden death is due to the fibrinolysis.</p

The continuance of ground availability by tuna purse seine fisheries in the Western Pacific Ocean

東京水産大学環境システム学講座釜慶大学校海洋生産管理学科水産大学校生産システム学講座東京水産大学環境システム学講