Genetic variation of Amaranthus retroflexus L. and Chenopodium album L. (Amaranthaceae) suggests multiple independent introductions into Iran

Amaranthus retroflexus L. and Chenopodium album L. (Amaranthaceae) are weedy plants that cause severe ecological and economic damage. In this study, we collected DNA from three different countries and assessed genetic diversity using inter-simple sequence repeat (ISSR) markers. Our analysis shows both weed species have low genetic diversity within a population and high genetic diversity among populations, as well as a low value of gene flow among the populations. UPGMA clustering and principal coordinate analysis indicate four distinct groups for A. retroflexus L. and C. album L. exist. We detected significant isolation-by-distance for A. retroflexus L. and no significant correlation for C. album L. These conclusions are based data from 13 ISSR primers where the average percentage of polymorphism produced was 98.46 % for A. retroflexus L. and 74.81% for C. album L.. These data suggest that each population was independently introduced to the location from which it was sampled and these noxious weeds come armed with considerable genetic variability giving them the opportunity to manifest myriad traits that could be used to avoid management practices. Our results, albeit not definitive about this issue, do not support the native status of C. album L. in Iran

Redroot pigweed (Amaranthus retroflexus L.) and lamb's quarters ‎‎(Chenopodium album L.) populations exhibit a high degree of ‎morphological and biochemical diversity

Amaranthus retroflexus L. and Chenopodium album L. are noxious weeds that have a cosmopolitan distribution. These species successfully invade and are adapted to a wide variety of diverse climates. In this paper we evaluated the morphology and biochemistry of 16 populations of A. retroflexus L. and 17 populations of C. album L.. Seeds from populations collected from Spain, France and Iran were grown together at the experimental field of the agriculture research of University of Mohaghegh Ardabili and a suite of morphological traits and biochemical traits were assessed. Among the populations of A. retroflexus L. and of C. album L. were observed significant differences for all the measured traits. The number of branches for A. retroflexus L. (12.22) and inflorescence length (14.34) for C. album L. were the two characteristics that exhibited the maximum coefficient of variation. Principal component analysis of these data identified four principal components for each species that explained 83.54 (A. retroflexus L.) and 88.98 (C. album L.) of the total variation. A dendrogram based on unweighted neighbor-joining method clustered all the A. retroflexus L. and C. album L. into two main clusters and four sub-clusters. Canonical correlation analysis was used to evaluate relationships between climate classification of origin and traits. Similarly, the measured characteristics did not group along Köppen climate classification. Both analyses support the conclusion that A. retroflexus L. and C. album L. exhibit high levels of diversity despite similar environmental histories. Both species also exhibit a high diversity of the measured biochemical compounds indicating they exhibit different metabolic profiles even when grown concurrently and sympatrically. Several of the biochemical constituents identified in our study could serve as effective indices for indirect selection of stresses resistance/tolerance of A. retroflexus L. and C. album L. The diversity of the morphological and biochemical traits observed among these populations illustrates how the unique selection pressures faced by each population can alter the biology of these plants. This understanding provides new insights to how these invasive plant species successfully colonize diverse ecosystems and suggests methods for their management under novel and changing environmental conditions

The Performance and Incidence of Ascites in Broiler Chickens in Response to Feed Restriction and Meal Feeding Programs

An experiment was conducted to evaluate the effect of feed restriction (FR) and meal feeding (MF) on performance, carcass characteristics and related parameters to ascites in Ross 308 male broiler chickens. Five dietary treatments were 1) the control (ad libo. feeding), 2 )feed restriction from 7 to 14 days (FR14), 3) feed restriction from 7 to 21 days (FR21), 4) meal feeding from 7 to 14 days (MF14) and 5) meal feeding from 7 to 21days (MF21). All birds were fed adlibitum for the first week post hatch and after 21 days of age. Four replicate of 12 birds were allocated to each treatment. The results indicated FR and MF21 groups had significantly (

Evaluating Rimsulforon Efficiency on Controlling Weeds in Potato at Different Growth Stages

Introduction Potato is globally the fourth important food crop after rice, wheat, and maize and is cultivated in several countries worldwide like Iran. Weeds are the most important restriction factor for crop development in agricultural systems and the absence of weed control lead to crop yield reduction between 10 to 100 percent and this reduction could be a serious threat for a growing world population to be considered. Rimsulfuron is a sulfonylurea herbicide for control of weeds in potato, corn, wheat, barley and canola. It can be used as pre or post emergence in potato. These herbicide act through inhibition of enzyme acetolactate synthase (ALS), which catalyzes key reactions in the biosynthesis of basic branched-chain amino acids that are essential components of the growth process in plant cell division such as valine, leucine, and isoleucine Materials and methods In order to study rimsulfuron (Titus 25% DF) effect on weed biomass as a postemergence herbicide, a field experiment was carried out in the farm located 3 km out the city of Ardabil during 2014. The factorial experiment was performed based on randomized complete block design with three replications. Potato cultivar was Agria (common cultivar in Ardabil). The first factor was rimsulfuron doses with six levels (5, 10, 20, 30, 40 and 50 gr a.i. / ha), and the second factor was time of application based on different potato growth stages, (Potato emergence, stoloning and tuber bulking). Also two treatment (with and without weeding) were considered as control. Tubers were hand sown on 22th May in rows 75 cm apart and 20 cm in the rows at 10 cm depth. Rimsulfuron was applied by backpack sprayer fitted with 8001 flat fan nozzles. Three weeks after treatment, weed sampling was carried out by a quadrate sized 0.375 m2. Tubers were harvested from two center rows to determine total tuber yield per hectare. Two models fitted to the data were three parameter logistic and three parameter sigmoid. Data were analyzed using SAS 9.1 and MSTAT-C. Analysis of variance was used to test the significance of variance sources, and Duncan’s Multiple range test (P ≤ 0.05) was used to compare the differences among treatments. Results and Discussion Results showed that the maximum reduction percent of redroot pigweed (Amaranthus retroflexus L.), field bind weed (Convolvulus arvensis L.), lambsquarter (Chenopodium album L.), total weed biomass and density achieved by 40 and 50 gr a.i. / ha application. So that 40 and 50 gr a.i. /ha of rimsulfuron compared to control reduced weed density up to 49.47and 55.38 % and biomass up to 89.97 and 94.63 %, respectively. Also results showed that 40 and 50 gr a.i. /ha of rimsulfuron compared to control reduced biomass of redroot pigweed 89.91 and 95.50 %, Field bind weed 84.28 and 94.79 % and lambsquarter 92.75 and 96.87 percent respectively. That there was not statistically significant difference between 40 and 50 gr a.i. /ha of rimsulfuron. Among rimsulfuron application times, potato emergence stage reduced total weed density and biomass 36.45 and 68.04 %, respectively which is compared to control (weedy). Rimsulfuron application at potato emergence stage reduced biomass of redroot pigweed and lambsquarter 62.51 and 71.08 percent respectively. However it had no significant effect on biomass of Field bind weed. The results showed that potato tuber yield was 46.31 and 45.96 ton/ ha while rimsulfuron applied 40 and 50 gr a.i. / ha. Among rimsulfuron application times, potato emergence stage had total tuber yield 47.58 ton/ha. Conclusion Results showed that the maximum reduction percentage in weed density and biomass was observed for treatments in which were used 40 and 50 gr a.i. / ha application. Application of rimsulfuron at potato emergence reduced weed density and biomass and increased total tuber yield. Itproves the effectiveness of the rimsulfuron for potato emergence without damage. Furthermore the effective dose of rimsulfuron for 50 percent reduction in biomass of redroot pigweed, field bind weed and lambsquarter were 14.36, 12.26 and 10.17 g a.i. /ha, respectively. The results also showed that rimsulfuron application 40 and 50 gr a.i. /ha increased total tuber yield by 19.90 and 20.51% respectively. Application of 40 and 50 gr a.i. / ha at potato emergence stage produced maximum total tuber yield per hectare. The result of this study showed that 40 and 50 gr a.i. /ha rimsulfuron application at potato emergence assessed as the best treatment to control weed and increase total yield

The relationship between morphological traits and seed yield of Iranian garden cress accession

In order to evaluate the relationship between traits affecting the seed yield of the garden cress, phenotypic correlations between seed yield and yield components were detected, direct and indirect effects of yield components on yield and its role in the description of diversity were estimated, and the experiment was conducted in a randomized complete block design with three replications in 2017. The ANOVA showed that the difference among accessions was significant for all traits. There was a significant phenotypic correlation between yield and most of the traits. The highest value of phenotypic correlation was obtained between seed yield and the ‘thousand seed weight’ (0.97). The regression analysis showed that the seed yield was highly influenced by the ‘thousand seed weight’, leaf height and the number of seeds per silique of the lateral branches and the main axis. These three traits constituted about 89% of changes in the total yield, while the ‘thousand seed weight’ was the most effective trait that entered the model and explained 51% of the variation. The path analysis of phenotypic correlation showed that the ‘thousand seed weight’ had the greatest direct effect on the yield (7.21). The ‘thousand seed weight’ had a positive indirect impact on other traits as well. As a result of the factor analysis, 3 independent factors explained about 79.33% of the yield variation. These factors consisted of the number of seeds per silique of the lateral branches, the number of seeds per silique of the main axis and the ‘thousand seed weight’

Root and shoot parts of strawberry: factories for production of functional human pro-insulin

Diabetes, a disease caused by excessive blood sugar, is caused by the lack of insulin. For commercial production, insulin is made in bacteria or yeast by protein recombinant technology. The focus of this research is evaluating another resource and producing of recombinant insulin protein in as strawberry as this plant has high potential in production of pharmaceutical proteins. Strawberry is a suitable bioreactor for production of recombinant proteins especially edible vaccines. In this research, human pro-insulin gene was cloned in pCAMBIA1304 vector under CaMV35S promoter and NOS terminator. Agrobacterium tumefaciens LBA4404, AGL1, EHA105, EHA101, C58, C58 (pGV2260) and C58 (pGV3101) strains were used for transformation of pro-insulin gene into strawberry cv. Camarosa, Selva, Sarian Hybrid, Pajaro, Paros, Gaviota, Alpine. Additionally, Agrobacterium rhizogenes K599, R1000, A4 and MSU440 strains were utilized for gene transformation into hairy roots. PCR analysis indicated the presence of transformed human pro-insulin gene in the strawberry and hairy roots. Also, its transcription was confirmed using RT-PCR. Furthermore, the analysis of plants, fruits and hairy roots at the level of proteins using dot blot, ELISA, SDS-PAGE and ECL tests re-confirmed the expression of this protein in the transgenic plants as well as hairy roots. Protein purification of human pro-insulin from transgenic tissues was performed using affinity chromatography. Finally, the bioassay of recombinant pro-insulin was performed. The analysis of second generations of transgenic plants (T1) at DNA and protein levels was also performed as a complementary experiment. This study opens a new avenue in molecular farming of human pro-insulin through its mass production in roots and shoots of strawberry.Ashkan Tavizi, Mokhtar Jalali Javaran, Ahmad Moieni, Manijeh Mohammadi-Dehcheshmeh, Mehdi Mohebodini, Esmaeil Ebrahimi

Synergistic effects of fermented soybean meal and mannan-oligosaccharide on growth performance, digestive functions, and hepatic gene expression in broiler chickens

This study investigated the effects of fermented soybean meal (FSBM) with or without mannan-oligosaccharide (MOS) prebiotic on growth performance, digestive functions, and hepatic IGF-1 gene expression of broiler chicken. A total of 480 day-old male broiler chickens were fed with 4 experimental diets for 6 wk. Experimental diets included corn-soybean meal diet (CON); corn-soybean meal diet + MOS prebiotic [0.2%, ActiveMOS; Biorigin, Brazile]; corn-FSBM diet [soybean meal (SBM) was totally replaced by FSBM]; and corn-FSBM + MOS prebiotic (MIX). Replacing dietary SBM with FSBM with or without MOS improved body weight gain and feed efficiency for the total grow-out period. However, the addition of MOS to the FSBM diet exhibited a greater body weight gain than other experimental diets. Villus height and villus height to crypt depth of the duodenum and jejunum were increased by feeding FSBM, MOS, and MIX diets. The ileal crude protein and energy digestibilities, as well as the activities of intestinal amylase and protease, and pancreatic protease, were improved by replacing SBM with FSBM, with or without MOS. The concentration of plasma 3-methylhistidine was reduced by FSBM and MOS, and synergistically by their combination. The MOS and FSBM diets upregulated the hepatic IGF-1 gene expression. However, there was an evident synergistic effect of FSBM supplemented with MOS in the upregulation of the hepatic IGF-1 gene expression. The outcomes of the current study indicate the FSBM and MOS had the potential to improve growth performance, hepatic IGF-1 expression, and intestinal morphology of broilers. Overall, the fermented products could be considered as functional feed that exhibits probiotic effects and the synergistic effects of prebiotics added to the fermented feeds may further improve the growth performance and gut health and functionality in broiler chicken