Insights into salt tolerance of mustard (Brassica juncea L. Czern & Coss): A metabolomics perspective

Salt stress is one of the key abiotic factor which leads to reduced global agricultural productions through negatively impacting the growth and development of crops. Indian mustard (Brassica juncea), the most important cruciferous crop with significant nutritional and medicinal values, is majorly affected by salt stress. In this study, we explored the global metabolomic response of two Indian mustard genotypes, CS 60 and CS 245–2–80–7 grown under salt stress for different time periods to unleash the role of differentially accumulated metabolites and relevant metabolic pathways involved in the salt tolerance mechanism. A total of 608 known compounds were detected from 4119 metabolites using DionexUltiMate® 3000 Ultra High-Performance Liquid Chromatographic System combined with “Q Exactive™ Plus Orbitrap™ Mass Spectrometer (UHPLC-MS/MS) analysis, from which 111 significantly altered metabolites in both genotypes were selected based on t-test and VIP score values. Using MetPa from MetaboAnalyst 5.0 platform, metabolic pathways with significant impact values were considered to be involved in the salt tolerance mechanism. Increased accumulation of metabolites and detected relevant pathways majorly regulating the anti-oxidant defense system gives CS 60, a high yielding variety, an edge against the genotype CS 245–2–80–7, which might be the chief tolerance mechanism to withstand salt stress

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Not AvailableComprehensive characterization of networks of gene expression regulators is key challenge of functional genomics. Soybean genomic sequences can be used to frame model for binding sites of fundamental transcription factors, and RNA seq data. It has been manifested that the reverse genetics approach can reveal gene regulator networks in soybean, which explains regulatory mechanisms for salt response from expression of gene patterns. In this study, the sequences of protein is functionally validated salt tractable genes is used to key out soybean orthologous with the help of ‘blastP’ search against Glycine max genome database. Thirty- eight salt responsive genes allocate among 39 cis-acting elements of soybean is elucidated by the ‘PlantCARE’ database. Gene promoter analysis of salt tractable genes indicated the presence of biotic and abiotic stress responsive, light, hormone, plant tissues specific and gene specific cis-regulatory elements in soybean. A total, 38 salt tractable genes in soybean is entrusted for GO terms based on the ‘Ensembl Plants’ database. These GO terms are sum up into the three main categories (biological process, cellular component, and molecular function) and 62 sub-categories. These GO annotations comprise a general profile signature of gene expression for salt tractable genes in soybean. Our results explained about the identified soybean salt tractable genes differentially respond in plant growth, development and gene regulation during biotic and abiotic stress conditions. The data obtained from this study contribute to a better enlightening of the role of the regulatory and functional pathway of salt tolerant genes in soybean.Not Availabl