60 research outputs found
Mesenchymal Stem Cells Promote Mammosphere Formation and Decrease E-Cadherin in Normal and Malignant Breast Cells
Normal and malignant breast tissue contains a rare population of multi-potent cells with the capacity to self-renew, referred to as stem cells, or tumor initiating cells (TIC). These cells can be enriched by growth as "mammospheres" in three-dimensional cultures.We tested the hypothesis that human bone-marrow derived mesenchymal stem cells (MSC), which are known to support tumor growth and metastasis, increase mammosphere formation.We found that MSC increased human mammary epithelial cell (HMEC) mammosphere formation in a dose-dependent manner. A similar increase in sphere formation was seen in human inflammatory (SUM149) and non-inflammatory breast cancer cell lines (MCF-7) but not in primary inflammatory breast cancer cells (MDA-IBC-3). We determined that increased mammosphere formation can be mediated by secreted factors as MSC conditioned media from MSC spheroids significantly increased HMEC, MCF-7 and SUM149 mammosphere formation by 6.4 to 21-fold. Mammospheres grown in MSC conditioned media had lower levels of the cell adhesion protein, E-cadherin, and increased expression of N-cadherin in SUM149 and HMEC cells, characteristic of a pro-invasive mesenchymal phenotype. Co-injection with MSC in vivo resulted in a reduced latency time to develop detectable MCF-7 and MDA-IBC-3 tumors and increased the growth of MDA-IBC-3 tumors. Furthermore, E-cadherin expression was decreased in MDA-IBC-3 xenografts with co-injection of MSC.MSC increase the efficiency of primary mammosphere formation in normal and malignant breast cells and decrease E-cadherin expression, a biologic event associated with breast cancer progression and resistance to therapy
Physical property characterization of single step synthesized NdFeAsO0.80F0.20 bulk 50K superconductor
We report an easy single step synthesis route of title compound
NdFeAsO0.80F0.20 superconductor having bulk superconductivity below 50 K. The
title compound is synthesized via solid-state reaction route by encapsulation
in an evacuated (10-3 Torr) quartz tube. Rietveld analysis of powder X-ray
diffraction data shows that compound crystallized in tetragonal structure with
space group P4/nmm. R(T)H measurements showed superconductivity with Tc (R=0)
at 48 K and a very high upper critical field (Hc2) of up to 345 Tesla. Magnetic
measurements exhibited bulk superconductivity in terms of diamagnetic onset
below 50 K. The lower critical field (Hc1) is around 1000 Oe at 5 K. In normal
state i.e., above 60 K, the compound exhibited purely paramagnetic behavior and
thus ruling out the presence of any ordered FeOx impurity in the matrix. In
specific heat measurements a jump is observed in the vicinity of
superconducting transition (Tc) along with an upturn at below T=4 K due to the
AFM ordering of Nd+3 ions in the system. The Thermo-electric power (TEP) is
negative down to Tc, thus indicating dominant carriers to be of n-type in
NdFeAsO0.80F0.20 superconductor. The granularity of the bulk superconducting
NdFeAsO0.8F0.2 sample is investigated and the intra and inter grain
contributions have been individuated by looking at various amplitude and
frequencies of the applied AC drive magnetic field.Comment: 26pages text + Figures: comments/suggestions welcome
([email protected] & http://www.freewebs.com/vpsawana
Intelligent prediction of aliphatic and aromatic hydrocarbons in Caspian Sea sediment using a neural network based on particle swarm optimization
Evaluation of human cytomegalovirus antigen expression in invasive breast carcinoma in a population of Iranian patients
Torsional behavior of RC beams strengthened with carbon fiber reinforced polymer composites
Effect of cooking methods on bioaccessibility of Zn, Se, Cd, Cu in sea cucumber (Apostichopus japonicus)
Preliminary observations on the plasma composition of liza klunzingeri from the strait of Hormuz (Persian gulf)
Reactions of 6âAminouracils: The First Simple, Fast, and Highly Efficient Synthesis of bis(6âAminopyrimidonyl)methanes (BAPMs) Using Thermal or MicrowaveâAssisted SolventâFree Methods
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