Effect of Plant Growth Hormones on Shoot and Root Regeneration in Rose under In Vitro Conditions

Background: Rose is a commercially important shrub. This research aimed to observe the influence of different plant growth hormones on development of shoots and roots of Rosa Indica L. in tissue culture.Methods: Various concentrations of N6-Benzylaminopurine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-acetic acid (IAA), and Indole-3-butyric acid (IBA) were used in the study. The different concentrations of BAP (2.00, 3.00, and 4.00 mg l-1) and IAA (2.00 and 3.00 mg l-1) were tested for shoot induction. While varying concentrations of IAA and IBA were analyzed for root proliferation.Results: The results of the study indicated that the fastest shoot initiation (17.77 days), the highest number of shoots bottle-1 (3.55), the maximum shoot length (4.72 cm), and the utmost number of leaves bottle-1 (53.67) were observed on MS media containing 3.00 mg l-1 BAP, 3.00 mg l-1 IAA, and 30 g l-1 sugar, while the highest number of shoots bottle-1 were produced under MS + 2.00 mg l-1 BAP + 3.00 mg l-1 IAA + 30 g l-1 sugar. Regarding root induction, the maximum number of roots (4.67) and root length (2.60 cm) were observed under half strength MS media supplemented with 30 g l-1 sugar.Conclusion: The study suggested that MS media containing 3.00 mg l-1 BAP and IAA could be used for tissue culturing rose plants. For root induction, half-strength MS media, along with sugar, could be used. The study gives an insight into potential media compositions for the propagation of rose. The suggested media can have promising uses in commercial multiplication of this important plant.Keywords: Rose; Tissue culture; Micropropagation; MS media; Shoot developmen

Efficiency of nitrogen, gibberellic acid and potassium on canola production under sub-tropical regions of Pakistan

Abstract The global demand for crop production is rapidly growing due to the continued rise in world population. Crop productivity varies generally with soil nutrient profile and climate. The optimal use of fertilizers might help to attain higher crop yield in canola. To circumvent nutrient imbalance issues in soil, two separate field trials were conducted to determine (a) the best source of nitrogen (N) between ammonium sulfate (NH4)2SO4) and ammonium nitrate (NH4NO3), (b) significance of gibberellic acid (GA3) and potassium (K), in an attempt to enhance canola yield and yield attributes. Both experiments were carried out in randomized complete block design (RCBD) with three replicates. The nitrogen source in the form of NH4)2SO4 (0, 10, 20 and 30 kg/ha) and NH4NO3 (0, 50, 75 and 100 kg/ha) was applied in the rhizosphere after 3 and 7 weeks of sowing, referred to as experiment 1 (E1). In another separate experiment (E2), the canola crop was sprayed with four level of GA3 (0, 10, 15, 30 g/ha) and K (0, 2.5, 3.5, 6 g/ha) individually or in combination by using hydraulic spryer, 30 days after sowing (DAS). The data was collected at different growth stages of canola and analyzed statistically. The E1 trail showed that N fortification in the form of NH4NO3 (100 kg/ha) and (NH4)2SO4 (30 kg/ha) had a positive effect on the plant height, number of branches, fruiting zone, seed yield per plant, seed yield per hectare of canola except oil percentage. Moreover, canola plants (E2) also displayed a significant improvement on all studied features with high doses of GA3 (30 g/ha) and K (6 g/ha) individualy and in combined form. The correlation coefficient analysis of (NH4)2SO4 and NH4NO3 was highly significant to plant height, number of branches, fruiting zone, seed yield per plant, seed yield per hectare of canola In a nutshell, compared to both source of N, NH4NO3 was more efficient and readily available source of N. GA3 being a growth elicitor and potassium as a micronutrient serve as potential source to improve yield and to manage nutrient profile of canola

Stroke in South Asian countries

Three of the world\u27s top 10 most populous countries are located in South Asia. The health-care problems of this region are different from those in the developed world, and the rapidly changing socioeconomic scenario, fast-increasing urbanization and longevity, changes in dietary patterns, and decrease in mortality from infectious diseases has made chronic illnesses of old age, such as coronary artery disease and stroke, an important area of focus. This article reviews stroke epidemiology and management issues in four South Asian countries: India, Pakistan, Sri Lanka and Bangladesh. The available literature is limited and mostly hospital-based, and differing study methodologies make direct comparisons difficult. The high prevalence of traditional risk factors, including hypertension, diabetes, dyslipidaemia and smoking, in these countries is alarming, and several nontraditional risk factors, such as water-pipe use, desi ghee, chewable tobacco, and infectious causes of stroke, are understudied. Access to tertiary stroke care is limited, and the use of tissue plasminogen activator is scarce. In addition, public and caregiver awareness of stroke risk factors and management is disappointing, and the interest of governments and policy makers in stroke is suboptimal. Interventions to reduce stroke burden and stroke-related mortality in South Asia should have a substantial impact at the global level