6 research outputs found

    Cell culture as a model for drug testing

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    The purpose of the study is to evaluate the cytotoxic effect of drugs using the ImageJ cell bioimaging program.ЦСль исслСдования - ΠΎΡ†Π΅Π½ΠΈΡ‚ΡŒ цитотоксичСскоС дСйствиС ΠΏΡ€Π΅ΠΏΠ°Ρ€Π°Ρ‚ΠΎΠ² с ΠΏΠΎΠΌΠΎΡ‰ΡŒΡŽ ΠΏΡ€ΠΎΠ³Ρ€Π°ΠΌΠΌΡ‹ для Π±ΠΈΠΎΠΈΠΌΠΈΠ΄ΠΆΠΈΠ½Π³Π° ΠΊΠ»Π΅Ρ‚ΠΎΠΊ Β«ImageJΒ»

    Evaluation of changes in estrogen expression level and KI-67 proliferation index during breast cancer cell culture, derived from a sample of luminal to HER-2 subtype negative

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    The purpose of the study was to determine the expression levels of estrogen receptors and the Ki67 cell proliferation index in a cell culture obtained from a sample of the luminal B HER-2 negative subtype over five passages.ЦСль исслСдования β€” ΠΎΠΏΡ€Π΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡƒΡ€ΠΎΠ²Π½Π΅ΠΉ экспрСсии Ρ€Π΅Ρ†Π΅ΠΏΡ‚ΠΎΡ€ΠΎΠ² эстрогСна ΠΈ индСкса ΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ ΠΏΡ€ΠΎΠ»ΠΈΡ„Π΅Ρ€Π°Ρ†ΠΈΠΈ Кi67 Π² ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π΅ ΠΊΠ»Π΅Ρ‚ΠΎΠΊ, ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Π½ΠΎΠΉ ΠΈΠ· ΠΎΠ±Ρ€Π°Π·Ρ†Π° люминального Π’ HER-2 Π½Π΅Π³Π°Ρ‚ΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΠΎΠ΄Ρ‚ΠΈΠΏΠ° Π½Π° протяТСнии пяти пассаТСй

    Cytological evaluation of a single cell culture of Luminal A subtype breast carcinoma cells

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    The aim of the study was to compare the morphological characteristics of the cells of a breast carcinoma sample when cultured over three passages.ЦСль исслСдования β€” ΡΡ€Π°Π²Π½ΠΈΡ‚ΡŒ морфологичСскиС характСристики ΠΊΠ»Π΅Ρ‚ΠΎΠΊ ΠΎΠ±Ρ€Π°Π·Ρ†Π° ΠΊΠ°Ρ€Ρ†ΠΈΠ½ΠΎΠΌΡ‹ ΠΌΠΎΠ»ΠΎΡ‡Π½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ‹ ΠΏΡ€ΠΈ ΠΊΡƒΠ»ΡŒΡ‚ΠΈΠ²ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠΈ Π½Π° протяТСнии Ρ‚Ρ€Π΅Ρ… пассаТС

    Determination of the epithelial nature of cells and the level of proliferative activity of Ki-67 in the primary culture of breast carcinoma

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    The aim of the study was to study the possibility of primary cell culture to maintain the proliferative ability and epithelial nature of cells over several passages.ЦСль исслСдования β€” ΠΈΠ·ΡƒΡ‡ΠΈΡ‚ΡŒ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡ‚ΡŒ ΠΏΠ΅Ρ€Π²ΠΈΡ‡Π½ΠΎΠΉ ΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½ΠΎΠΉ ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Ρ‹ ΡΠΎΡ…Ρ€Π°Π½ΡΡ‚ΡŒ ΠΏΡ€ΠΎΠ»ΠΈΡ„Π΅Ρ€Π°Ρ‚ΠΈΠ²Π½ΡƒΡŽ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡ‚ΡŒ ΠΈ ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½ΡƒΡŽ ΠΏΡ€ΠΈΡ€ΠΎΠ΄Ρƒ ΠΊΠ»Π΅Ρ‚ΠΎΠΊ Π½Π° протяТСнии Π½Π΅ΡΠΊΠΎΠ»ΡŒΠΊΠΈΡ… пассаТСй

    Comparison of adherent and non-adherent cell phenotypes of breast carcinoma cultures. Ural medical journal

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    The aim of the study was to determine the effect of the method of culturing BC cells on the ability of cells to preserve the epithelial phenotype.ЦСль исслСдования – ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΠΈΡ‚ΡŒ влияниС ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ ΠΊΡƒΠ»ΡŒΡ‚ΠΈΠ²ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΡ ΠΊΠ»Π΅Ρ‚ΠΎΠΊ ΠšΠœΠ– Π½Π° ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡ‚ΡŒ ΠΊΠ»Π΅Ρ‚ΠΎΠΊ ΡΠΎΡ…Ρ€Π°Π½ΡΡ‚ΡŒ ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½Ρ‹ΠΉ Ρ„Π΅Π½ΠΎΡ‚ΠΈΠΏ

    Morphological characteristics of cells obtained from breast carcinoma samples of different molecular biological subtypes

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    The article presents the morphological characteristics of cells obtained from samples of human breast carcinoma Luminal A, HER2 + and Triple negative molecular biological subtypes during cultivation. The analysis showed that in the first passage cells of the epithelial phenotype are found, which indicates the epithelial nature of the tumors and is confirmed by the detection of the immunocytochemical marker pancytokeratin. The identification of fusiform, The article presents the morphological characteristics of cells obtained from samples of human breast carcinoma Luminal A, HER2 + and Triple negative molecular biological subtypes during cultivation. The analysis showed that in the first passage cells of the epithelial phenotype are found, which indicates the epithelial nature of the tumors and is confirmed by the detection of the immunocytochemical marker pancytokeratin. The identification of fusiform,Π’ ΡΡ‚Π°Ρ‚ΡŒΠ΅ Π΄Π°Π½Π° морфологичСская характСристика ΠΊΠ»Π΅Ρ‚ΠΎΠΊ, ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Π½Ρ‹Ρ… ΠΈΠ· ΠΎΠ±Ρ€Π°Π·Ρ†ΠΎΠ² ΠΊΠ°Ρ€Ρ†ΠΈΠ½ΠΎΠΌΡ‹ ΠΌΠΎΠ»ΠΎΡ‡Π½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ‹ Ρ‡Π΅Π»ΠΎΠ²Π΅ΠΊΠ° Luminal А, HER2+ ΠΈ Π’Ρ€ΠΎΠΉΠ½ΠΎΠ³ΠΎ Π½Π΅Π³Π°Ρ‚ΠΈΠ²Π½ΠΎΠ³ΠΎ молСкулярно-биологичСских ΠΏΠΎΠ΄Ρ‚ΠΈΠΏΠΎΠ², Π² Ρ…ΠΎΠ΄Π΅ ΠΊΡƒΠ»ΡŒΡ‚ΠΈΠ²ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΡ. Анализ ΠΏΠΎΠΊΠ°Π·Π°Π», Ρ‡Ρ‚ΠΎ Π² ΠΏΠ΅Ρ€Π²ΠΎΠΌ пассаТС ΠΎΠ±Π½Π°Ρ€ΡƒΠΆΠΈΠ²Π°ΡŽΡ‚ΡΡ ΠΊΠ»Π΅Ρ‚ΠΊΠΈ ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½ΠΎΠ³ΠΎ Ρ„Π΅Π½ΠΎΡ‚ΠΈΠΏΠ°, Ρ‡Ρ‚ΠΎ ΡΠ²ΠΈΠ΄Π΅Ρ‚Π΅Π»ΡŒΡΡ‚Π²ΡƒΠ΅Ρ‚ ΠΎΠ± ΡΠΏΠΈΡ‚Π΅Π»ΠΈΠ°Π»ΡŒΠ½ΠΎΠΉ ΠΏΡ€ΠΈΡ€ΠΎΠ΄Π΅ ΠΎΠΏΡƒΡ…ΠΎΠ»Π΅ΠΉ ΠΈ подтвСрТдаСтся выявлСниСм иммуноцитохимичСского ΠΌΠ°Ρ€ΠΊΠ΅Ρ€Π° ΠΏΠ°Π½Ρ†ΠΈΡ‚ΠΎΠΊΠ΅Ρ€Π°Ρ‚ΠΈΠ½. ВыявлСниС Π² ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π°Ρ… Π²Π΅Ρ€Π΅Ρ‚Π΅Π½ΠΎΠ²ΠΈΠ΄Π½Ρ‹Ρ…, фибробластоподобных ΠΈ ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΎΠΏΠΎΠ΄ΠΎΠ±Π½Ρ‹Ρ… ΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Ρ… Ρ„Π΅Π½ΠΎΡ‚ΠΈΠΏΠΎΠ² ΡΠ²ΠΈΠ΄Π΅Ρ‚Π΅Π»ΡŒΡΡ‚Π²ΡƒΡŽΡ‚ ΠΎ гСтСрогСнности ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Ρ‹ ΠΎΠΏΡƒΡ…ΠΎΠ»Π΅Π²Ρ‹Ρ… ΠΊΠ»Π΅Ρ‚ΠΎ
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