6 research outputs found
Cell culture as a model for drug testing
The purpose of the study is to evaluate the cytotoxic effect of drugs using the ImageJ cell bioimaging program.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ - ΠΎΡΠ΅Π½ΠΈΡΡ ΡΠΈΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ Π΄Π»Ρ Π±ΠΈΠΎΠΈΠΌΠΈΠ΄ΠΆΠΈΠ½Π³Π° ΠΊΠ»Π΅ΡΠΎΠΊ Β«ImageJΒ»
Evaluation of changes in estrogen expression level and KI-67 proliferation index during breast cancer cell culture, derived from a sample of luminal to HER-2 subtype negative
The purpose of the study was to determine the expression levels of estrogen receptors and the Ki67 cell proliferation index in a cell culture obtained from a sample of the luminal B HER-2 negative subtype over five passages.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Π΅ΠΉ ΡΠΊΡΠΏΡΠ΅ΡΠΈΠΈ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ² ΡΡΡΡΠΎΠ³Π΅Π½Π° ΠΈ ΠΈΠ½Π΄Π΅ΠΊΡΠ° ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ Πi67 Π² ΠΊΡΠ»ΡΡΡΡΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΠΎΠΉ ΠΈΠ· ΠΎΠ±ΡΠ°Π·ΡΠ° Π»ΡΠΌΠΈΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π HER-2 Π½Π΅Π³Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΠΎΠ΄ΡΠΈΠΏΠ° Π½Π° ΠΏΡΠΎΡΡΠΆΠ΅Π½ΠΈΠΈ ΠΏΡΡΠΈ ΠΏΠ°ΡΡΠ°ΠΆΠ΅ΠΉ
Cytological evaluation of a single cell culture of Luminal A subtype breast carcinoma cells
The aim of the study was to compare the morphological characteristics of the cells of a breast carcinoma sample when cultured over three passages.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΡΡΠ°Π²Π½ΠΈΡΡ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΎΠ±ΡΠ°Π·ΡΠ° ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΠΏΡΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Π½Π° ΠΏΡΠΎΡΡΠΆΠ΅Π½ΠΈΠΈ ΡΡΠ΅Ρ
ΠΏΠ°ΡΡΠ°ΠΆΠ΅
Determination of the epithelial nature of cells and the level of proliferative activity of Ki-67 in the primary culture of breast carcinoma
The aim of the study was to study the possibility of primary cell culture to maintain the proliferative ability and epithelial nature of cells over several passages.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΠΈΠ·ΡΡΠΈΡΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΡ ΡΠΎΡ
ΡΠ°Π½ΡΡΡ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΡ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ ΠΈ ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ ΠΏΡΠΈΡΠΎΠ΄Ρ ΠΊΠ»Π΅ΡΠΎΠΊ Π½Π° ΠΏΡΠΎΡΡΠΆΠ΅Π½ΠΈΠΈ Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΈΡ
ΠΏΠ°ΡΡΠ°ΠΆΠ΅ΠΉ
Comparison of adherent and non-adherent cell phenotypes of breast carcinoma cultures. Ural medical journal
The aim of the study was to determine the effect of the method of culturing BC cells on the ability of cells to preserve the epithelial phenotype.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΠΠ Π½Π° ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠΎΡ
ΡΠ°Π½ΡΡΡ ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΠΉ ΡΠ΅Π½ΠΎΡΠΈΠΏ
Morphological characteristics of cells obtained from breast carcinoma samples of different molecular biological subtypes
The article presents the morphological characteristics of cells obtained from samples of human breast carcinoma Luminal A, HER2 + and Triple negative molecular biological subtypes during cultivation. The analysis showed that in the first passage cells of the epithelial phenotype are found, which indicates the epithelial nature of the tumors and is confirmed by the detection of the immunocytochemical marker pancytokeratin. The identification of fusiform, The article presents the morphological characteristics of cells obtained from samples of human breast carcinoma Luminal A, HER2 + and Triple negative molecular biological subtypes during cultivation. The analysis showed that in the first passage cells of the epithelial phenotype are found, which indicates the epithelial nature of the tumors and is confirmed by the detection of the immunocytochemical marker pancytokeratin. The identification of fusiform,Π ΡΡΠ°ΡΡΠ΅ Π΄Π°Π½Π° ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΈΠ· ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Luminal Π, HER2+ ΠΈ Π’ΡΠΎΠΉΠ½ΠΎΠ³ΠΎ Π½Π΅Π³Π°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠΎΠ΄ΡΠΈΠΏΠΎΠ², Π² Ρ
ΠΎΠ΄Π΅ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ. ΠΠ½Π°Π»ΠΈΠ· ΠΏΠΎΠΊΠ°Π·Π°Π», ΡΡΠΎ Π² ΠΏΠ΅ΡΠ²ΠΎΠΌ ΠΏΠ°ΡΡΠ°ΠΆΠ΅ ΠΎΠ±Π½Π°ΡΡΠΆΠΈΠ²Π°ΡΡΡΡ ΠΊΠ»Π΅ΡΠΊΠΈ ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅Π½ΠΎΡΠΈΠΏΠ°, ΡΡΠΎ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΠ΅Ρ ΠΎΠ± ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Π΅ ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ ΠΈ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π°Π΅ΡΡΡ Π²ΡΡΠ²Π»Π΅Π½ΠΈΠ΅ΠΌ ΠΈΠΌΠΌΡΠ½ΠΎΡΠΈΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ°ΡΠΊΠ΅ΡΠ° ΠΏΠ°Π½ΡΠΈΡΠΎΠΊΠ΅ΡΠ°ΡΠΈΠ½. ΠΡΡΠ²Π»Π΅Π½ΠΈΠ΅ Π² ΠΊΡΠ»ΡΡΡΡΠ°Ρ
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ΠΈ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠΏΠΎΠ΄ΠΎΠ±Π½ΡΡ
ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
ΡΠ΅Π½ΠΎΡΠΈΠΏΠΎΠ² ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ Π³Π΅ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΠΎΡΡΠΈ ΠΊΡΠ»ΡΡΡΡΡ ΠΎΠΏΡΡ
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