6 research outputs found
The EURO-NOTES clinical registry for natural orifice transluminal endoscopic surgery: a 2-year activity report
Magnetic resonance imaging radiomic feature analysis of radiation-induced femoral head changes in prostate cancer radiotherapy
DOG1 overexpression is associated with mismatch repair deficiency and BRAF mutations but unrelated to cancer progression in colorectal cancer
Introduction. The transmembrane channel
protein DOG1 (Discovered on GIST1) is normally
expressed in the gastrointestinal interstitial cells of Cajal
and also in gastrointestinal stroma tumors arising from
these cells. However, there is also evidence for a
relevant role of DOG1 expression in colorectal cancers.
This study was undertaken to search for associations
between DOG1 expression and colon cancer phenotype
and key molecular alterations.
Methods. A tissue microarray containing samples
from more than 1,800 colorectal cancer patients was
analyzed by immunohistochemistry.
Results. DOG1 immunostaining was detected in 503
(30.2%) of 1,666 analyzable colorectal cancers and
considered weak in 360 (21.6%), moderate in 78 (4.7%),
and strong in 65 (3.9%). Strong DOG1 immunostaining
was associated with advanced pT stage (p=0.0367) and
nodal metastases (p=0.0145) but these associations were
not retained in subgroups of 1,135 mismatch repair
proficient and 86 mismatch repair deficient tumors.
DOG1 positivity was significantly linked to several
molecular tumor features including mismatch repair
deficiency (p=0.0034), BRAF mutations (p<0.0001),
nuclear p53 accumulation (p=0.0157), and PD-L1
expression (p=0.0199) but unrelated to KRAS mutations
and the density of tumor infiltrating CD8 positive
lymphocytes.
Conclusion. Elevated DOG1 expression is frequent
in colorectal cancer and significantly linked to important
molecular alterations. However, DOG1 overexpression
is largely unrelated to histopathological parameters of
cancer aggressiveness and may thus not serve as a
prognostic parameter for this tumor entity
Elevated MUC5AC expression is associated with mismatch repair deficiency and proximal tumor location but not with cancer progression in colon cancer
Mucin 5AC (MUC5AC) is a secreted gel-forming mucin expressed by several epithelia. In the colon, MUC5AC is expressed in scattered normal epithelial cells but can be abundant in colorectal cancers. To clarify the relationship of MUC5AC expression with parameters of tumor aggressiveness and mismatch repair deficiency (dMMR) in colorectal cancer, a tissue microarray containing 1812 colorectal cancers was analyzed by immunohistochemistry. MUC5AC expression was found in 261 (15.7%) of 1,667 analyzable colorectal cancers. MUC5AC expression strongly depended on the tumor location and gradually decreased from proximal (27.4% of cecum cancers) to distal (10.6% of rectal cancers; p &lt; 0.0001). MUC5AC expression was also strongly linked to dMMR. dMMR was found in 21.3% of 169 cancers with MUC5AC positivity but in only 4.6% of 1051 cancers without detectable MUC5AC expression (p &lt; 0.0001). A multivariate analysis showed that dMMR status and tumor localization predicted MUC5AC expression independently (p &lt; 0.0001 each). MUC5AC expression was unrelated to pT and pN status. This also applied to the subgroups of 1136 proficient MMR (pMMR) and of 84 dMMR cancers. The results of our study show a strong association of MUC5AC expression with proximal and dMMR colorectal cancers. However, MUC5AC expression is unrelated to colon cancer aggressiveness